################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccII AccIII AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstPI BstUI BstYI Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MboI MfeI MflI MluI MroI MseI MspI MunI MvnI NaeI NarI NciI NdeI NdeII NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 691 GTATAC GT^[AC][GT]AC AciI 357 CCGC CCGC(-3/-1) AclI 81 AACGTT AA^CGTT AspHI 416 GTGCTC G[AT]GC[AT]^C AspI 259 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 425 TGGCCA TGG^CCA BanI 813 GGCACC G^G[CT][GA]CC BbsI 256 GAAGAC GAAGAC(2/6) BglI 340 GCCTCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 416 GTGCTC G[AGT]GC[ACT]^C BpmI 783 CTCCAG CTGGAG(16/14) BpuAI 256 GAAGAC GAAGAC(2/6) BsiHKAI 416 GTGCTC G[AT]GC[AT]^C BsiYI 468 CCACACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 468 CCACACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG Bsp1286I 416 GTGCTC G[AGT]GC[ACT]^C BspMI 63 GCAGGT ACCTGC(4/8) BsrDI 729 CATTGC GCAATG(2/0) Bst1107I 691 GTATAC GTA^TAC BstAPI 280 GCATGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 230 CCATCGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 691 GTATAC GTA^TAC Cac8I 578 GCGAGC GC[ACGT]^[ACGT]GC DsaI 794 CCATGG C^C[GA][CT]GG EaeI 425 TGGCCA [CT]^GGCC[GA] EcoRV 639 GATATC GAT^ATC EcoT14I 794 CCATGG C^C[AT][AT]GG EcoT22I 278 ATGCAT ATGCA^T HphI 662 TCACC GGTGA(8/7) MaeII 82 ACGT A^CGT MluNI 425 TGGCCA TGG^CCA MscI 425 TGGCCA TGG^CCA MspA1I 218 CAGCTG C[AC]G^C[GT]G NcoI 794 CCATGG C^CATGG NsiI 278 ATGCAT ATGCA^T NspI 280 GCATGT [GA]CATG^[CT] PflFI 259 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 52 GACTC GAGTC(4/5) Psp1406I 81 AACGTT AA^CGTT PstI 61 CTGCAG CTGCA^G PvuII 218 CAGCTG CAG^CTG SfiI 339 GGCCTCAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StyI 794 CCATGG C^C[AT][AT]GG Tth111I 259 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 785 CCAGTCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 171 GTAC GT^AC 768 GTAC BbvI 364 GCAGC GCAGC(8/12) 286 GCTGC BfaI 37 CTAG C^TAG 196 CTAG BsaJI 120 CCTGGG C^C[ACGT][ACGT]GG 794 CCATGG BsmFI 476 GGGAC GGGAC(10/14) 751 GGGAC BsmI 456 GAATGC GAATGC(1/-1) 892 GCATTC BsrI 472 ACTGG ACTGG(1/-1) 785 CCAGT BssKI 120 CCTGG ^CC[ACGT]GG 627 CCTGG BstNI 120 CCTGG CC^[AT]GG 627 CCTGG EarI 213 CTCTTC CTCTTC(1/4) 885 GAAGAG EcoRII 120 CCTGG ^CC[AT]GG 627 CCTGG Fnu4HI 286 GCTGC GC^[ACGT]GC 364 GCAGC ItaI 286 GCTGC GC^[ACGT]GC 364 GCAGC Ksp632I 213 CTCTTC CTCTTC(1/4) 885 GAAGAG MaeI 37 CTAG C^TAG 196 CTAG MvaI 120 CCTGG CC^[AT]GG 627 CCTGG RsaI 171 GTAC GT^AC 768 GTAC ScrFI 120 CCTGG CC^[ACGT]GG 627 CCTGG SfaNI 675 GATGC GCATC(5/9) 740 GCATC TfiI 148 GATTC G^A[AT]TC 245 GATTC TseI 286 GCTGC G^C[AT]GC 364 GCAGC Tsp45I 223 GTCAC ^GT[GC]AC 788 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AluI BsmAI BstF5I DdeI FokI HaeIII HinfI MaeIII MboII MnlI MslI MwoI NlaIII NlaIV SfcI Tsp509I TspRI