################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MflI MluI MluNI MroI MscI MseI MspA1I MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SspBI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AciI 447 GCGG CCGC(-3/-1) AocI 838 CCTGAGG CC^T[ACGT]AGG AvaII 386 GGACC G^G[AT]CC BanI 934 GGTGCC G^G[CT][GA]CC BanII 166 GGGCTC G[GA]GC[CT]^C BbsI 3 GAAGAC GAAGAC(2/6) BbvI 326 GCTGC GCAGC(8/12) BciVI 192 GGATAC GTATCC(6/5) BclI 617 TGATCA T^GATCA BpuAI 3 GAAGAC GAAGAC(2/6) BsaAI 195 TACGTA [CT]AC^GT[GA] BsaBI 100 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BstF5I 99 GGATG GGATG(2/0) BstXI 275 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 838 CCTGAGG CC^T[ACGT]AGG DraIII 403 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 389 CCATGG C^C[GA][CT]GG Eco81I 838 CCTGAGG CC^T[ACGT]AGG EcoRI 70 GAATTC G^AATTC EcoT14I 389 CCATGG C^C[AT][AT]GG EcoT22I 40 ATGCAT ATGCA^T FbaI 617 TGATCA T^GATCA FokI 99 GGATG GGATG(9/13) HinfI 303 GAGTC G^A[ACGT]TC MaeII 196 ACGT A^CGT MamI 100 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 532 CAATTG C^AATTG MunI 532 CAATTG C^AATTG MwoI 96 GCAGGATGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 389 CCATGG C^CATGG NsiI 40 ATGCAT ATGCA^T PflMI 402 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 303 GAGTC GAGTC(4/5) SapI 537 GCTCTTC GCTCTTC(1/4) SexAI 143 ACCTGGT A^CC[AT]GGT SfcI 906 CTACAG C^T[GA][CT]AG SmlI 912 CTTGAG C^T[CT][GA]AG SnaBI 195 TACGTA TAC^GTA StyI 389 CCATGG C^C[AT][AT]GG TseI 326 GCTGC G^C[AT]GC Van91I 402 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AcsI 70 GAATTC [GA]^AATT[CT] 962 AAATTT ApoI 70 GAATTC [GA]^AATT[CT] 962 AAATTT BmyI 166 GGGCTC G[AGT]GC[ACT]^C 935 GTGCCC BseRI 115 CTCCTC GAGGAG(10/8) 825 CTCCTC BsiYI 402 CCACTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 451 CCCAGGTTCGG BslI 402 CCACTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 451 CCCAGGTTCGG BsmFI 385 GGGAC GGGAC(10/14) 271 GTCCC Bsp1286I 166 GGGCTC G[AGT]GC[ACT]^C 935 GTGCCC EarI 220 CTCTTC CTCTTC(1/4) 538 CTCTTC Fnu4HI 326 GCTGC GC^[ACGT]GC 447 GCGGC ItaI 326 GCTGC GC^[ACGT]GC 447 GCGGC Ksp632I 220 CTCTTC CTCTTC(1/4) 538 CTCTTC SspI 289 AATATT AAT^ATT 496 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AfaI AluI Asp700I BsaJI BssKI BstNI Cfr13I DdeI DpnII EcoRII HaeIII HphI MaeIII MboI MboII MnlI MslI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI Tsp45I Tsp509I TspRI XmnI