################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MflI MluI MluNI MroI MscI MseI MspA1I MspI MvnI MwoI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TfiI Tru9I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AciI 447 GCGG CCGC(-3/-1) AocI 838 CCTGAGG CC^T[ACGT]AGG AvaII 386 GGACC G^G[AT]CC BanI 934 GGTGCC G^G[CT][GA]CC BanII 166 GGGCTC G[GA]GC[CT]^C BbsI 3 GAAGAC GAAGAC(2/6) BbvI 326 GCTGC GCAGC(8/12) BclI 617 TGATCA T^GATCA BpuAI 3 GAAGAC GAAGAC(2/6) BsiYI 402 CCACTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 402 CCACTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BssSI 480 CTCGTG CACGAG(-5/-1) BstF5I 725 CATCC GGATG(2/0) BstXI 275 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 838 CCTGAGG CC^T[ACGT]AGG Cac8I 447 GCGGGC GC[ACGT]^[ACGT]GC DraIII 403 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 389 CCATGG C^C[GA][CT]GG Eco81I 838 CCTGAGG CC^T[ACGT]AGG EcoRI 70 GAATTC G^AATTC EcoT14I 389 CCATGG C^C[AT][AT]GG EcoT22I 40 ATGCAT ATGCA^T FauI 447 GCGGG CCCGC(4/6) FbaI 617 TGATCA T^GATCA Fnu4HI 326 GCTGC GC^[ACGT]GC FokI 725 CATCC GGATG(9/13) HinfI 86 GACTC G^A[ACGT]TC ItaI 326 GCTGC GC^[ACGT]GC NcoI 389 CCATGG C^CATGG NsiI 40 ATGCAT ATGCA^T PflMI 402 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 86 GACTC GAGTC(4/5) SapI 537 GCTCTTC GCTCTTC(1/4) SmlI 912 CTTGAG C^T[CT][GA]AG StyI 389 CCATGG C^C[AT][AT]GG TaqI 398 TCGA T^CGA TseI 326 GCTGC G^C[AT]GC TthHB8I 398 TCGA T^CGA Van91I 402 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AcsI 70 GAATTC [GA]^AATT[CT] 962 AAATTT ApoI 70 GAATTC [GA]^AATT[CT] 962 AAATTT BmyI 166 GGGCTC G[AGT]GC[ACT]^C 935 GTGCCC BseRI 115 CTCCTC GAGGAG(10/8) 825 CTCCTC Bsp1286I 166 GGGCTC G[AGT]GC[ACT]^C 935 GTGCCC EarI 220 CTCTTC CTCTTC(1/4) 538 CTCTTC Ksp632I 220 CTCTTC CTCTTC(1/4) 538 CTCTTC MfeI 532 CAATTG C^AATTG 776 CAATTG MunI 532 CAATTG C^AATTG 776 CAATTG SfcI 803 CTGTAG C^T[GA][CT]AG 906 CTACAG SspI 289 AATATT AAT^ATT 496 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AfaI AluI Asp700I BsaJI BsmFI BssKI BstNI Cfr13I DdeI DpnII EcoRII HaeIII HphI MaeIII MboI MboII MnlI MslI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI Tsp45I Tsp509I TspRI XmnI