################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MflI MluI MroI MseI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AciI 696 CCGC CCGC(-3/-1) AlwNI 701 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 710 AGCGCT AGC^GCT Asp700I 717 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 427 GGCACC G^G[CT][GA]CC BanII 908 GAGCTC G[GA]GC[CT]^C BbsI 838 GTCTTC GAAGAC(2/6) BfaI 565 CTAG C^TAG BpuAI 838 GTCTTC GAAGAC(2/6) BsaI 174 GAGACC GGTCTC(1/5) BsmFI 945 GGGAC GGGAC(10/14) BspHI 44 TCATGA T^CATGA BspMI 430 ACCTGC ACCTGC(4/8) BsrBI 696 CCGCTC CCGCTC(-3/-3) BsrDI 651 CATTGC GCAATG(2/0) BstEII 801 GGTGACC G^GT[ACGT]ACC BstPI 801 GGTGACC G^GT[ACGT]ACC BstXI 242 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 711 GCGC GCG^C DpnII 373 GATC ^GATC DrdI 195 GACACACTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 362 CCATGG C^C[GA][CT]GG Eco47III 710 AGCGCT AGC^GCT EcoO65I 801 GGTGACC G^GT[ACGT]ACC HaeII 710 AGCGCT [GA]GCGC^[CT] HhaI 711 GCGC GCG^C HinP1I 711 GCGC G^CGC HinfI 920 GAGTC G^A[ACGT]TC MaeI 565 CTAG C^TAG MboI 373 GATC ^GATC NcoI 362 CCATGG C^CATGG NdeII 373 GATC ^GATC NheI 564 GCTAGC G^CTAGC PflMI 14 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 920 GAGTC GAGTC(4/5) RcaI 44 TCATGA T^CATGA SacI 908 GAGCTC GAGCT^C SapI 312 GCTCTTC GCTCTTC(1/4) Sau3AI 373 GATC ^GATC SfcI 879 CTACAG C^T[GA][CT]AG StuI 719 AGGCCT AGG^CCT TaqI 918 TCGA T^CGA TthHB8I 918 TCGA T^CGA Van91I 14 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 137 CCATCATTTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 717 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcsI 37 GAATTT [GA]^AATT[CT] 129 GAATTT AfaI 108 GTAC GT^AC 222 GTAC ApoI 37 GAATTT [GA]^AATT[CT] 129 GAATTT AspHI 576 GTGCTC G[AT]GC[AT]^C 908 GAGCTC AvaII 23 GGACC G^G[AT]CC 159 GGACC BalI 134 TGGCCA TGG^CCA 383 TGGCCA BlpI 698 GCTCAGC GC^T[ACGT]AGC 706 GCTGAGC Bpu1102I 698 GCTCAGC GC^T[ACGT]AGC 706 GCTGAGC BsgI 688 CTGCAC GTGCAG(16/14) 734 GTGCAG BsiHKAI 576 GTGCTC G[AT]GC[AT]^C 908 GAGCTC CelII 698 GCTCAGC GC^T[ACGT]AGC 706 GCTGAGC EaeI 134 TGGCCA [CT]^GGCC[GA] 383 TGGCCA MluNI 134 TGGCCA TGG^CCA 383 TGGCCA MscI 134 TGGCCA TGG^CCA 383 TGGCCA MspA1I 446 CAGCTG C[AC]G^C[GT]G 701 CAGCTG NlaIV 23 GGACCC GG[ACGT]^[ACGT]CC 427 GGCACC PvuII 446 CAGCTG CAG^CTG 701 CAGCTG RsaI 108 GTAC GT^AC 222 GTAC Tsp509I 38 AATT ^AATT 130 AATT TspRI 236 CAGTG CA[GC]TG[ACGT][ACGT]^ 683 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AluI BbvI BmyI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HphI ItaI Ksp632I MaeIII MboII MnlI MslI MvaI MwoI NlaIII Sau96I ScrFI SfaNI StyI TseI Tsp45I