(Circular) (Six-base) MAPSORT of: pFN21AE6037.seq Check: 8186 from: 1 to: 5656 pFN21AE6037 5656 bp With 182 enzymes: SgfI * December 8, 2014 13:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2444 2444 Size: 5656 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 1937 Size: 1062 4594 Acc65I G'GTAC_C Cuts at: 2977 2977 Size: 5656 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3719 5103 5371 1632 Size: 2087 1384 268 1917 Fragments arranged by size: 2087 1917 1384 268 AfeI AGC'GCT Cuts at: 2717 2717 Size: 5656 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 4555 5532 1203 Size: 3352 977 1327 Fragments arranged by size: 3352 1327 977 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 4385 4385 Size: 5656 AleI CACnn'nnGTG Cuts at: 1166 2126 1166 Size: 960 4696 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5656 AlwNI CAG_nnn'CTG Cuts at: 2711 3399 4971 2711 Size: 688 1572 3396 Fragments arranged by size: 3396 1572 688 ApaLI G'TGCA_C Cuts at: 3713 4869 3713 Size: 1156 4500 ApoI r'AATT_y Cuts at: 1493 2042 2134 2965 3191 3343 5452 1493 Size: 549 92 831 226 152 2109 1697 Fragments arranged by size: 2109 1697 831 549 226 152 92 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2981 1870 Size: 73 1038 4545 Fragments arranged by size: 4545 1038 73 BamHI G'GATC_C Cuts at: 2986 3353 2986 Size: 367 5289 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2432 2977 4432 618 Size: 325 217 627 645 545 1455 1842 Fragments arranged by size: 1842 1455 645 627 545 325 217 BanII G_rGCy'C Cuts at: 1391 2917 2975 1391 Size: 1526 58 4072 Fragments arranged by size: 4072 1526 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2836 928 Size: 971 937 3748 Fragments arranged by size: 3748 971 937 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3847 3881 1903 Size: 34 50 34 1826 34 3678 Fragments arranged by size: 3678 1826 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3548 4758 3548 Size: 1210 4446 BclI T'GATC_A Cuts at: 1548 1548 Size: 5656 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4267 136 Size: 107 122 71 631 605 2595 1525 Fragments arranged by size: 2595 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5651 5651 Size: 5656 BlpI GC'TnA_GC Cuts at: 2704 2712 3073 2704 Size: 8 361 5287 Fragments arranged by size: 5287 361 8 Bme1580I G_kGCm'C Cuts at: 1790 2247 2435 3717 4873 1790 Size: 457 188 1282 1156 2573 Fragments arranged by size: 2573 1282 1156 457 188 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5656 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3387 4345 442 Size: 416 53 83 399 24 1970 958 1753 Fragments arranged by size: 1970 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2573 1056 Size: 1517 4139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4316 1239 Size: 3077 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3781 4646 4944 3781 Size: 865 298 4493 Fragments arranged by size: 4493 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2173 4319 882 Size: 617 674 2146 2219 Fragments arranged by size: 2219 2146 674 617 BsaAI yAC'GTr Cuts at: 493 1204 3447 493 Size: 711 2243 2702 Fragments arranged by size: 2702 2243 711 BsaBI GATnn'nnATC Cuts at: 5650 5650 Size: 5656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3463 3845 4480 275 Size: 53 83 186 936 1930 382 635 1451 Fragments arranged by size: 1930 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4089 4487 4761 4908 5319 4089 Size: 398 274 147 411 4426 Fragments arranged by size: 4426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2531 2561 1178 Size: 30 165 30 1128 30 4273 Fragments arranged by size: 4273 1128 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2635 2803 1186 Size: 889 560 168 4039 Fragments arranged by size: 4039 889 560 168 BseYI C'CCAG_C Cuts at: 2313 4859 5355 2313 Size: 2546 496 2614 Fragments arranged by size: 2614 2546 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2678 2760 1163 Size: 573 942 82 4059 Fragments arranged by size: 4059 942 573 82 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3106 3867 4895 1540 Size: 275 186 1105 761 1028 2301 Fragments arranged by size: 2301 1105 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2585 2917 3717 3802 4873 2585 Size: 332 800 85 1071 3368 Fragments arranged by size: 3368 1071 800 332 85 BsmI GAATG_Cn' Cuts at: 3167 3260 3167 Size: 93 5563 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2247 2435 2585 2917 2975 3717 3802 Size: 399 457 188 150 332 58 742 85 Cuts at: 3802 4873 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 457 399 332 188 150 85 58 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 2049 3545 5275 2049 Size: 1496 1730 2430 Fragments arranged by size: 2430 1730 1496 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2444 3011 844 Size: 1600 567 3489 Fragments arranged by size: 3489 1600 567 BsrBI CCG'CTC Cuts at: 1394 1823 2703 3543 1394 Size: 429 880 840 3507 Fragments arranged by size: 3507 880 840 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2655 4151 4325 65 Size: 1183 1407 1496 174 1396 Fragments arranged by size: 1496 1407 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4300 4487 1700 Size: 110 2490 187 2869 Fragments arranged by size: 2869 2490 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5656 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5656 BssSI C'ACGA_G Cuts at: 3716 4728 3716 Size: 1012 4644 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5656 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 2806 1184 Size: 1622 4034 BstXI CCAn_nnnn'nTGG Cuts at: 2254 2254 Size: 5656 BstYI r'GATC_y Cuts at: 1972 2986 3353 3738 3755 5207 5293 5305 5651 Size: 1014 367 385 17 1452 86 12 346 Cuts at: 5651 1972 Size: 1977 Fragments arranged by size: 1977 1452 1014 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2367 513 Size: 552 1302 3802 Fragments arranged by size: 3802 1302 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4917 Fragments arranged by size: 4917 683 56 BtsI GCAGTG_nn' Cuts at: 2687 3172 3966 3986 2687 Size: 485 794 20 4357 Fragments arranged by size: 4357 794 485 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5621 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2206 4663 809 Size: 507 890 2457 1802 Fragments arranged by size: 2457 1802 890 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2139 2388 3103 Size: 54 1425 50 275 18 309 249 715 Cuts at: 3103 3992 5621 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 715 309 275 249 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3103 1537 Size: 275 1291 4090 Fragments arranged by size: 4090 1291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 2105 2324 2771 3586 1353 Size: 740 12 219 447 815 3423 Fragments arranged by size: 3423 815 740 447 219 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4243 4617 4763 387 Size: 1064 138 2654 374 146 1280 Fragments arranged by size: 2654 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2915 2915 Size: 5656 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3719 4316 5103 5371 1239 Size: 393 2087 597 787 268 1524 Fragments arranged by size: 2087 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FspI TGC'GCA Cuts at: 4162 4162 Size: 5656 HaeII r_GCGC'y Cuts at: 1678 2719 4803 1678 Size: 1041 2084 2531 Fragments arranged by size: 2531 2084 1041 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2531 2563 4297 4329 4371 Size: 32 135 32 959 32 1734 32 42 Cuts at: 4371 4403 1373 Size: 32 2626 Fragments arranged by size: 2626 1734 959 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3000 3246 669 Size: 988 281 1062 246 3079 Fragments arranged by size: 3079 1062 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5656 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KpnI G_GTAC'C Cuts at: 2981 2981 Size: 5656 MfeI C'AATT_G Cuts at: 3255 3255 Size: 5656 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4770 4954 1305 Size: 3465 184 2007 Fragments arranged by size: 3465 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2141 2390 10 Size: 54 1425 343 309 249 3276 Fragments arranged by size: 3276 1425 343 309 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 2126 2150 2396 2660 2864 3614 3973 Size: 648 960 24 246 264 204 750 359 Cuts at: 3973 4132 518 Size: 159 2042 Fragments arranged by size: 2042 960 750 648 359 264 246 204 159 24 MspA1I CmG'CkG Cuts at: 1606 2453 2708 3072 3749 4897 5142 1606 Size: 847 255 364 677 1148 245 2120 Fragments arranged by size: 2120 1148 847 677 364 255 245 NcoI C'CATG_G Cuts at: 513 1065 2367 513 Size: 552 1302 3802 Fragments arranged by size: 3802 1302 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NheI G'CTAG_C Cuts at: 1052 2569 1052 Size: 1517 4139 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 3014 4559 1132 Size: 97 1785 1545 2229 Fragments arranged by size: 2229 1785 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5656 PciI A'CATG_T Cuts at: 4555 4555 Size: 5656 PflMI CCAn_nnn'nTGG Cuts at: 1108 2025 1108 Size: 917 4739 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3725 3757 5238 5270 3725 Size: 32 1481 32 4111 Fragments arranged by size: 4111 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5656 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PstI C_TGCA'G Cuts at: 830 3008 830 Size: 2178 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 PvuII CAG'CTG Cuts at: 2453 2708 2453 Size: 255 5401 SacI G_AGCT'C Cuts at: 2917 2917 Size: 5656 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SapI GCTCTTCn'nnn_ Cuts at: 2324 2324 Size: 5656 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SfcI C'TryA_G Cuts at: 826 1045 2884 3004 4139 4820 5011 826 Size: 219 1839 120 1135 681 191 1471 Fragments arranged by size: 1839 1471 1135 681 219 191 120 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2983 1872 Size: 1111 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3760 4661 4923 820 Size: 197 926 1817 901 262 1553 Fragments arranged by size: 1817 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 2726 1517 Size: 1209 4447 StyI C'CwwG_G Cuts at: 513 1065 2124 2367 2802 3095 513 Size: 552 1059 243 435 293 3074 Fragments arranged by size: 3074 1059 552 435 293 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2497 3683 3700 3853 4038 4483 325 Size: 1255 917 1186 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1186 917 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3902 5614 96 Size: 274 80 33 51 1443 1925 1712 138 Fragments arranged by size: 1925 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5616 5648 5616 Size: 32 5624 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5656 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2149 2149 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5656 XmaI C'CCGG_G Cuts at: 1870 2981 1870 Size: 1111 4545 XmnI GAAnn'nnTTC Cuts at: 2726 3785 2726 Size: 1059 4597 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI