################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AhdI Alw44I AlwNI AocI ApaI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraII DraIII DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AciI 535 GCGG CCGC(-3/-1) AcsI 68 AAATTC [GA]^AATT[CT] AgeI 359 ACCGGT A^CCGGT AlwI 204 GGATC GGATC(4/5) Aor51HI 453 AGCGCT AGC^GCT ApoI 68 AAATTC [GA]^AATT[CT] AspHI 417 GTGCTC G[AT]GC[AT]^C BanI 435 GGTGCC G^G[CT][GA]CC BbvI 395 GCAGC GCAGC(8/12) BcnI 57 CCGGG CC^[GC]GG BfaI 493 CTAG C^TAG BglII 329 AGATCT A^GATCT BmyI 417 GTGCTC G[AGT]GC[ACT]^C BpmI 241 CTGGAG CTGGAG(16/14) BsaBI 330 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 472 GAGACC GGTCTC(1/5) BsaWI 359 ACCGGT [AT]^CCGG[AT] BseRI 73 CTCCTC GAGGAG(10/8) BsiHKAI 417 GTGCTC G[AT]GC[AT]^C BsmAI 472 GAGAC GTCTC(1/5) BsmFI 42 GGGAC GGGAC(10/14) Bsp1286I 417 GTGCTC G[AGT]GC[ACT]^C BsrFI 359 ACCGGT [GA]^CCGG[CT] BstXI 95 CCATTTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 454 GCGC GCG^C Cfr10I 359 ACCGGT [GA]^CCGG[CT] DraI 672 TTTAAA TTT^AAA DrdI 44 GACTGACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 453 AGCGCT AGC^GCT EcoNI 138 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Fnu4HI 395 GCAGC GC^[ACGT]GC HaeII 453 AGCGCT [GA]GCGC^[CT] HhaI 454 GCGC GCG^C HinP1I 454 GCGC G^CGC HindIII 704 AAGCTT A^AGCTT HphI 21 GGTGA GGTGA(8/7) ItaI 395 GCAGC GC^[ACGT]GC MaeI 493 CTAG C^TAG MaeII 16 ACGT A^CGT MamI 330 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 734 CAGCTG C[AC]G^C[GT]G NciI 57 CCGGG CC^[GC]GG NdeI 636 CATATG CA^TATG NspI 164 ACATGC [GA]CATG^[CT] PflMI 149 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 359 ACCGGT A^CCGGT PleI 228 GACTC GAGTC(4/5) PshAI 732 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 734 CAGCTG CAG^CTG SfaNI 237 GATGC GCATC(5/9) SfcI 686 CTACAG C^T[GA][CT]AG TseI 395 GCAGC G^C[AT]GC Van91I 149 CCAACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AfaI 174 GTAC GT^AC 363 GTAC AseI 517 ATTAAT AT^TAAT 897 ATTAAT AsnI 517 ATTAAT AT^TAAT 897 ATTAAT BalI 92 TGGCCA TGG^CCA 368 TGGCCA BsgI 12 CTGCAC GTGCAG(16/14) 292 GTGCAG BsrI 460 ACTGG ACTGG(1/-1) 50 CCAGT BstF5I 141 CATCC GGATG(2/0) 336 CATCC BstYI 204 GGATCT [GA]^GATC[CT] 329 AGATCT DpnII 205 GATC ^GATC 330 GATC EaeI 92 TGGCCA [CT]^GGCC[GA] 368 TGGCCA EcoT14I 123 CCTTGG C^C[AT][AT]GG 314 CCTTGG FokI 141 CATCC GGATG(9/13) 336 CATCC HapII 57 CCGG C^CGG 360 CCGG HpaII 57 CCGG C^CGG 360 CCGG MboI 205 GATC ^GATC 330 GATC MflI 204 GGATCT [GA]^GATC[CT] 329 AGATCT MluNI 92 TGGCCA TGG^CCA 368 TGGCCA MscI 92 TGGCCA TGG^CCA 368 TGGCCA MspI 57 CCGG C^CGG 360 CCGG MwoI 159 GCTCCACATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 343 GCTGTGATGGC NdeII 205 GATC ^GATC 330 GATC PshBI 517 ATTAAT AT^TAAT 897 ATTAAT RsaI 174 GTAC GT^AC 363 GTAC Sau3AI 205 GATC ^GATC 330 GATC StyI 123 CCTTGG C^C[AT][AT]GG 314 CCTTGG TfiI 678 GATTC G^A[AT]TC 793 GAATC XhoII 204 GGATCT [GA]^GATC[CT] 329 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AluI AvaII BsaJI BsiYI BslI BssKI BstNI Cac8I Cfr13I DdeI EarI EcoRII HaeIII HinfI Ksp632I MaeIII MboII MnlI MseI MslI MvaI NlaIII NlaIV Sau96I ScrFI Tru9I Tsp45I Tsp509I TspRI