Restriction map for
pFN21AE6054
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI AlwI AlwNI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BcnI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstYI BstZ17I CelII
CfoI Cfr10I Cfr13I CpoI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FokI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MflI MluI MroI
MspA1I MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI Sau96I SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AflIII 671 ACATGT A^C[GA][CT]GT
Alw44I 277 GTGCAC G^TGCAC
AocI 891 CCTAAGG CC^T[ACGT]AGG
ApaLI 277 GTGCAC G^TGCAC
AseI 606 ATTAAT AT^TAAT
AsnI 606 ATTAAT AT^TAAT
Asp700I 49 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 686 TGGCCA TGG^CCA
BanII 349 GAGCTC G[GA]GC[CT]^C
BbvI 84 GCTGC GCAGC(8/12)
BciVI 560 GTATCC GTATCC(6/5)
BsaBI 333 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiYI 94 CCACTGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 94 CCACTGTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 775 GGGAC GGGAC(10/14)
BsmI 322 GCATTC GAATGC(1/-1)
BspDI 938 ATCGAT AT^CGAT
BspLU11I 671 ACATGT A^CATGT
BsrI 809 CCAGT ACTGG(1/-1)
BssKI 79 CCTGG ^CC[ACGT]GG
BstNI 79 CCTGG CC^[AT]GG
Bsu36I 891 CCTAAGG CC^T[ACGT]AGG
ClaI 938 ATCGAT AT^CGAT
DraI 508 TTTAAA TTT^AAA
EaeI 686 TGGCCA [CT]^GGCC[GA]
Eco81I 891 CCTAAGG CC^T[ACGT]AGG
EcoRII 79 CCTGG ^CC[AT]GG
EcoT14I 38 CCAAGG C^C[AT][AT]GG
Fnu4HI 84 GCTGC GC^[ACGT]GC
ItaI 84 GCTGC GC^[ACGT]GC
MamI 333 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 686 TGGCCA TGG^CCA
MscI 686 TGGCCA TGG^CCA
MvaI 79 CCTGG CC^[AT]GG
MwoI 140 GCAATCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 318 GCTAGC G^CTAGC
NlaIV 90 GGCTCC GG[ACGT]^[ACGT]CC
NspI 671 ACATGT [GA]CATG^[CT]
PflMI 94 CCACTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 209 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 606 ATTAAT AT^TAAT
PstI 85 CTGCAG CTGCA^G
SacI 349 GAGCTC GAGCT^C
SapI 639 GCTCTTC GCTCTTC(1/4)
ScaI 435 AGTACT AGT^ACT
ScrFI 79 CCTGG CC^[ACGT]GG
SfaNI 913 GATGC GCATC(5/9)
SmlI 705 CTCAAG C^T[CT][GA]AG
SnaBI 385 TACGTA TAC^GTA
StyI 38 CCAAGG C^C[AT][AT]GG
TaqI 939 TCGA T^CGA
TseI 84 GCTGC G^C[AT]GC
TthHB8I 939 TCGA T^CGA
Van91I 94 CCACTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 809 CCAGTCATACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 49 GAATTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AcsI 49 GAATTT [GA]^AATT[CT]
947 AAATTC
ApoI 49 GAATTT [GA]^AATT[CT]
947 AAATTC
AspHI 277 GTGCAC G[AT]GC[AT]^C
349 GAGCTC
BclI 416 TGATCA T^GATCA
761 TGATCA
BfaI 319 CTAG C^TAG
682 CTAG
BmyI 277 GTGCAC G[AGT]GC[ACT]^C
349 GAGCTC
BsaAI 385 TACGTA [CT]AC^GT[GA]
412 TACGTG
BsiHKAI 277 GTGCAC G[AT]GC[AT]^C
349 GAGCTC
Bsp1286I 277 GTGCAC G[AGT]GC[ACT]^C
349 GAGCTC
BsrDI 246 CATTGC GCAATG(2/0)
367 GCAATG
Cac8I 87 GCAGGC GC[ACGT]^[ACGT]GC
318 GCTAGC
FbaI 416 TGATCA T^GATCA
761 TGATCA
HphI 42 GGTGA GGTGA(8/7)
759 GGTGA
MaeI 319 CTAG C^TAG
682 CTAG
MfeI 300 CAATTG C^AATTG
653 CAATTG
MunI 300 CAATTG C^AATTG
653 CAATTG
PleI 104 GACTC GAGTC(4/5)
172 GACTC
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AfaI AluI BsaJI DdeI DpnII
EarI HaeIII HinfI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MslI NdeII NlaIII RsaI Sau3AI
SfcI TfiI Tru9I Tsp45I Tsp509I
TspRI