(Circular) (Six-base) MAPSORT of: pFN21AE6054.seq Check: 9516 from: 1 to: 5662 pFN21AE6054 5662 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3472 278 Size: 53 83 186 936 1936 2468 Fragments arranged by size: 2468 1936 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3005 1937 Size: 1068 4594 Acc65I G'GTAC_C Cuts at: 2983 2983 Size: 5662 AclI AA'CG_TT Cuts at: 1563 3789 4162 1563 Size: 2226 373 3063 Fragments arranged by size: 3063 2226 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3725 5109 5377 1632 Size: 2093 1384 268 1917 Fragments arranged by size: 2093 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5465 AflIII A'CryG_T Cuts at: 1203 2676 4561 5538 1203 Size: 1473 1885 977 1327 Fragments arranged by size: 1885 1473 1327 977 AgeI A'CCGG_T Cuts at: 4493 4493 Size: 5662 AhdI GACnn_n'nnGTC Cuts at: 4391 4391 Size: 5662 AleI CACnn'nnGTG Cuts at: 1166 2036 2483 1166 Size: 870 447 4345 Fragments arranged by size: 4345 870 447 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5662 AlwNI CAG_nnn'CTG Cuts at: 3405 4977 3405 Size: 1572 4090 ApaLI G'TGCA_C Cuts at: 2282 3719 4875 2282 Size: 1437 1156 3069 Fragments arranged by size: 3069 1437 1156 ApoI r'AATT_y Cuts at: 1493 2054 2952 2971 3197 3349 5458 1493 Size: 561 898 19 226 152 2109 1697 Fragments arranged by size: 2109 1697 898 561 226 152 19 AseI AT'TA_AT Cuts at: 160 1032 2612 4216 160 Size: 872 1580 1604 1606 Fragments arranged by size: 1606 1604 1580 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 AvaI C'yCGr_G Cuts at: 1870 1943 2987 1870 Size: 73 1044 4545 Fragments arranged by size: 4545 1044 73 BamHI G'GATC_C Cuts at: 2992 3359 2992 Size: 367 5295 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2983 4438 618 Size: 325 217 627 1196 1455 1842 Fragments arranged by size: 1842 1455 1196 627 325 217 BanII G_rGCy'C Cuts at: 1391 2358 2981 1391 Size: 967 623 4072 Fragments arranged by size: 4072 967 623 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4691 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3853 3887 1903 Size: 34 1916 34 3678 Fragments arranged by size: 3678 1916 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2576 3554 4764 2576 Size: 978 1210 3474 Fragments arranged by size: 3474 1210 978 BclI T'GATC_A Cuts at: 1548 2421 2766 1548 Size: 873 345 4444 Fragments arranged by size: 4444 873 345 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4273 136 Size: 107 122 71 631 605 2601 1525 Fragments arranged by size: 2601 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5657 5657 Size: 5662 BlpI GC'TnA_GC Cuts at: 3079 3079 Size: 5662 Bme1580I G_kGCm'C Cuts at: 1790 2286 3723 4879 1790 Size: 496 1437 1156 2573 Fragments arranged by size: 2573 1437 1156 496 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5662 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3393 4351 442 Size: 416 53 83 399 24 1976 958 1753 Fragments arranged by size: 1976 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2327 1056 Size: 1271 4391 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4322 1239 Size: 3083 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2695 3787 4652 4950 2695 Size: 1092 865 298 3407 Fragments arranged by size: 3407 1092 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4325 882 Size: 617 2826 2219 Fragments arranged by size: 2826 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2392 2419 3453 493 Size: 711 1188 27 1034 2702 Fragments arranged by size: 2702 1188 1034 711 27 BsaBI GATnn'nnATC Cuts at: 2342 5656 2342 Size: 3314 2348 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3469 3851 4486 275 Size: 53 83 186 936 1936 382 635 1451 Fragments arranged by size: 1936 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4095 4493 4767 4914 5325 4095 Size: 398 274 147 411 4432 Fragments arranged by size: 4432 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2162 2192 1178 Size: 30 165 30 759 30 4648 Fragments arranged by size: 4648 759 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5662 BseYI C'CCAG_C Cuts at: 4865 5361 4865 Size: 496 5166 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5089 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3112 3873 4901 1540 Size: 275 186 1111 761 1028 2301 Fragments arranged by size: 2301 1111 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2286 2358 3723 3808 4879 2286 Size: 72 1365 85 1071 3069 Fragments arranged by size: 3069 1365 1071 85 72 BsmI GAATG_Cn' Cuts at: 2327 3173 3266 2327 Size: 846 93 4723 Fragments arranged by size: 4723 846 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2286 2358 2981 3723 3808 4879 1391 Size: 399 496 72 623 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 623 496 399 85 72 BspEI T'CCGG_A Cuts at: 5325 5325 Size: 5662 BspHI T'CATG_A Cuts at: 3551 5281 3551 Size: 1730 3932 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3017 844 Size: 2173 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3549 1394 Size: 429 1726 3507 Fragments arranged by size: 3507 1726 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2250 2379 4157 4331 65 Size: 1183 1002 129 1778 174 1396 Fragments arranged by size: 1778 1396 1183 1002 174 129 BsrFI r'CCGG_y Cuts at: 1700 1810 4306 4493 1700 Size: 110 2496 187 2869 Fragments arranged by size: 2869 2496 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5662 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5662 BssSI C'ACGA_G Cuts at: 3722 4734 3722 Size: 1012 4650 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5662 BstBI TT'CG_AA Cuts at: 4466 4466 Size: 5662 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5662 BstYI r'GATC_y Cuts at: 1972 2992 3359 3744 3761 5213 5299 5311 5657 Size: 1020 367 385 17 1452 86 12 346 Cuts at: 5657 1972 Size: 1977 Fragments arranged by size: 1977 1452 1020 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2897 2897 Size: 5662 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5110 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4923 Fragments arranged by size: 4923 683 56 BtsI GCAGTG_nn' Cuts at: 2043 3178 3972 3992 2043 Size: 1135 794 20 3713 Fragments arranged by size: 3713 1135 794 20 ClaI AT'CG_AT Cuts at: 1554 2944 1554 Size: 1390 4272 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2233 2268 529 Size: 35 1669 35 3923 Fragments arranged by size: 3923 1669 35 35 DraI TTT'AAA Cuts at: 2515 2966 2515 Size: 451 5211 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4669 809 Size: 507 3353 1802 Fragments arranged by size: 3353 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2691 3109 3998 Size: 54 1425 50 275 18 861 418 889 Cuts at: 3998 5627 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 861 418 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3109 1537 Size: 275 1297 4090 Fragments arranged by size: 4090 1297 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2117 2415 2651 3592 1353 Size: 764 298 236 941 3423 Fragments arranged by size: 3423 941 764 298 236 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4249 4623 4769 387 Size: 1064 138 2660 374 146 1280 Fragments arranged by size: 2660 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2356 2356 Size: 5662 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3725 4322 5109 5377 1239 Size: 393 2093 597 787 268 1524 Fragments arranged by size: 2093 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2971 3349 2971 Size: 378 5284 FspI TGC'GCA Cuts at: 4168 4168 Size: 5662 HaeII r_GCGC'y Cuts at: 1678 4809 1678 Size: 3131 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2100 2131 2132 2163 4303 Size: 32 135 32 528 31 1 31 2140 Cuts at: 4303 4335 4377 4409 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2140 528 135 42 32 32 32 32 31 31 1 HincII GTy'rAC Cuts at: 669 1657 1938 3006 3252 669 Size: 988 281 1068 246 3079 Fragments arranged by size: 3079 1068 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5662 HpaI GTT'AAC Cuts at: 3252 3252 Size: 5662 KpnI G_GTAC'C Cuts at: 2987 2987 Size: 5662 MfeI C'AATT_G Cuts at: 2305 2658 3261 2305 Size: 353 603 4706 Fragments arranged by size: 4706 603 353 MluI A'CGCG_T Cuts at: 5538 5538 Size: 5662 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4776 4960 1305 Size: 3471 184 2007 Fragments arranged by size: 3471 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2693 10 Size: 54 1425 343 861 2979 Fragments arranged by size: 2979 1425 861 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2036 2195 2483 3620 3979 4138 518 Size: 648 870 159 288 1137 359 159 2042 Fragments arranged by size: 2042 1137 870 648 359 288 159 159 MspA1I CmG'CkG Cuts at: 1606 3078 3755 4903 5148 1606 Size: 1472 677 1148 245 2120 Fragments arranged by size: 2120 1472 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5110 NdeI CA'TA_TG Cuts at: 387 387 Size: 5662 NheI G'CTAG_C Cuts at: 1052 2323 1052 Size: 1271 4391 NotI GC'GGCC_GC Cuts at: 3109 3109 Size: 5662 NspI r_CATG'y Cuts at: 1132 1229 2680 3020 4565 1132 Size: 97 1451 340 1545 2229 Fragments arranged by size: 2229 1545 1451 340 97 PasI CC'CwG_GG Cuts at: 1348 2084 1348 Size: 736 4926 PciI A'CATG_T Cuts at: 2676 4561 2676 Size: 1885 3777 PflMI CCAn_nnn'nTGG Cuts at: 1108 2105 1108 Size: 997 4665 PmeI GTTT'AAAC Cuts at: 2966 2966 Size: 5662 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3731 3763 5244 5276 3731 Size: 32 1481 32 4117 Fragments arranged by size: 4117 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2218 1613 Size: 605 5057 PsiI TTA'TAA Cuts at: 3232 3232 Size: 5662 PstI C_TGCA'G Cuts at: 830 2094 3014 830 Size: 1264 920 3478 Fragments arranged by size: 3478 1264 920 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5662 SacI G_AGCT'C Cuts at: 2358 2358 Size: 5662 SalI G'TCGA_C Cuts at: 1936 3004 1936 Size: 1068 4594 SapI GCTCTTCn'nnn_ Cuts at: 2651 2651 Size: 5662 SbfI CC_TGCA'GG Cuts at: 3014 3014 Size: 5662 ScaI AGT'ACT Cuts at: 2442 3910 5622 2442 Size: 1468 1712 2482 Fragments arranged by size: 2482 1712 1468 SfcI C'TryA_G Cuts at: 826 1045 2090 2473 2743 2890 3010 4145 4826 Size: 219 1045 383 270 147 120 1135 681 Cuts at: 4826 5017 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1045 681 383 270 219 191 147 120 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5662 SmaI CCC'GGG Cuts at: 1872 2989 1872 Size: 1117 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2710 3766 4667 4929 820 Size: 197 926 767 1056 901 262 1553 Fragments arranged by size: 1553 1056 926 901 767 262 197 SnaBI TAC'GTA Cuts at: 493 2392 3453 493 Size: 1899 1061 2702 Fragments arranged by size: 2702 1899 1061 SpeI A'CTAG_T Cuts at: 152 152 Size: 5662 SphI G_CATG'C Cuts at: 1132 3020 1132 Size: 1888 3774 SspI AAT'ATT Cuts at: 5 52 3586 5 Size: 47 3534 2081 Fragments arranged by size: 3534 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5662 StyI C'CwwG_G Cuts at: 513 1065 2043 3101 513 Size: 552 978 1058 3074 Fragments arranged by size: 3074 1058 978 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2437 3689 3706 3859 4044 4489 325 Size: 1255 857 1252 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1252 857 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2199 2440 3908 Size: 274 80 33 51 1443 222 241 1468 Cuts at: 3908 5620 96 Size: 1712 138 Fragments arranged by size: 1712 1468 1443 274 241 222 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5622 5654 5622 Size: 32 5630 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5662 XbaI T'CTAG_A Cuts at: 2998 2998 Size: 5662 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2821 2011 Size: 810 4852 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5662 XmaI C'CCGG_G Cuts at: 1870 2987 1870 Size: 1117 4545 XmnI GAAnn'nnTTC Cuts at: 2058 3791 2058 Size: 1733 3929 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3470 276 Size: 53 83 186 936 1936 2468 Fragments arranged by size: 2468 1936 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI