################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 711 GTCTAC GT^[AC][GT]AC AciI 147 CCGC CCGC(-3/-1) AflIII 536 ACACGT A^C[GA][CT]GT AlwI 807 GGATC GGATC(4/5) AlwNI 53 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 436 GGGCCC GGGCC^C AseI 679 ATTAAT AT^TAAT AsnI 679 ATTAAT AT^TAAT Asp700I 328 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanII 436 GGGCCC G[GA]GC[CT]^C BmyI 436 GGGCCC G[AGT]GC[ACT]^C BsaAI 364 TACGTA [CT]AC^GT[GA] BsmAI 540 GTCTC GTCTC(1/5) BsmBI 539 CGTCTC CGTCTC(1/5) Bsp1286I 436 GGGCCC G[AGT]GC[ACT]^C BspHI 587 TCATGA T^CATGA BsrDI 122 GCAATG GCAATG(2/0) BstXI 611 CCACCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 422 GCGC GCG^C DraI 774 TTTAAA TTT^AAA DraII 436 GGGCCCT [GA]G^G[ACGT]CC[CT] EarI 61 GAAGAG CTCTTC(1/4) EcoO109I 436 GGGCCCT [GA]G^G[ACGT]CC[CT] HhaI 422 GCGC GCG^C HinP1I 422 GCGC G^CGC HindIII 704 AAGCTT A^AGCTT HphI 740 TCACC GGTGA(8/7) Ksp632I 61 GAAGAG CTCTTC(1/4) PshBI 679 ATTAAT AT^TAAT PstI 375 CTGCAG CTGCA^G RcaI 587 TCATGA T^CATGA SfaNI 643 GCATC GCATC(5/9) SfcI 375 CTGCAG C^T[GA][CT]AG SnaBI 364 TACGTA TAC^GTA TspRI 432 CACTG CA[GC]TG[ACGT][ACGT]^ XmnI 328 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AvaII 66 GGTCC G^G[AT]CC 153 GGACC BbsI 917 GAAGAC GAAGAC(2/6) 310 GTCTTC BbvI 284 GCTGC GCAGC(8/12) 322 GCTGC BpmI 157 CTCCAG CTGGAG(16/14) 927 CTCCAG BpuAI 917 GAAGAC GAAGAC(2/6) 310 GTCTTC BsmFI 45 GGGAC GGGAC(10/14) 624 GTCCC BsrI 433 ACTGG ACTGG(1/-1) 394 CCAGT BssKI 150 CCTGG ^CC[ACGT]GG 929 CCAGG BstNI 150 CCTGG CC^[AT]GG 929 CCAGG DpnII 358 GATC ^GATC 808 GATC EcoRII 150 CCTGG ^CC[AT]GG 929 CCAGG Fnu4HI 284 GCTGC GC^[ACGT]GC 322 GCTGC ItaI 284 GCTGC GC^[ACGT]GC 322 GCTGC MboI 358 GATC ^GATC 808 GATC MvaI 150 CCTGG CC^[AT]GG 929 CCAGG NdeII 358 GATC ^GATC 808 GATC NlaIV 436 GGGCCC GG[ACGT]^[ACGT]CC 607 GGTTCC Sau3AI 358 GATC ^GATC 808 GATC ScrFI 150 CCTGG CC^[ACGT]GG 929 CCAGG TseI 284 GCTGC G^C[AT]GC 322 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AluI BstF5I Cfr13I DdeI FokI HaeIII HinfI MaeII MaeIII MboII MnlI MseI MslI NlaIII PleI Sau96I TfiI Tru9I Tsp45I Tsp509I