################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsiYI BslI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AciI 150 CCGC CCGC(-3/-1) AfaI 323 GTAC GT^AC AlwI 69 GATCC GGATC(4/5) AseI 682 ATTAAT AT^TAAT AsnI 682 ATTAAT AT^TAAT Asp700I 705 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 156 GGACC G^G[AT]CC BbsI 920 GAAGAC GAAGAC(2/6) BpuAI 920 GAAGAC GAAGAC(2/6) BsaJI 800 CCTTGG C^C[ACGT][ACGT]GG BseRI 345 CTCCTC GAGGAG(10/8) BsgI 564 CTGCAC GTGCAG(16/14) BsmAI 724 GTCTC GTCTC(1/5) BsmBI 723 CGTCTC CGTCTC(1/5) BsrI 436 ACTGG ACTGG(1/-1) BstXI 614 CCACCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 68 AGATCC [GA]^GATC[CT] DraI 777 TTTAAA TTT^AAA DrdI 463 GACTCCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT14I 800 CCTTGG C^C[AT][AT]GG HgaI 722 GCGTC GACGC(5/10) HincII 472 GTCAAC GT[CT]^[GA]AC HindII 472 GTCAAC GT[CT]^[GA]AC HindIII 707 AAGCTT A^AGCTT MflI 68 AGATCC [GA]^GATC[CT] NlaIV 610 GGTTCC GG[ACGT]^[ACGT]CC PshBI 682 ATTAAT AT^TAAT PstI 378 CTGCAG CTGCA^G RsaI 323 GTAC GT^AC SfaNI 646 GCATC GCATC(5/9) SfcI 378 CTGCAG C^T[GA][CT]AG StyI 800 CCTTGG C^C[AT][AT]GG XhoII 68 AGATCC [GA]^GATC[CT] XmnI 705 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BbvI 370 GCAGC GCAGC(8/12) 287 GCTGC BpmI 160 CTCCAG CTGGAG(16/14) 930 CTCCAG BsmFI 48 GGGAC GGGAC(10/14) 627 GTCCC BssKI 153 CCTGG ^CC[ACGT]GG 932 CCAGG BstNI 153 CCTGG CC^[AT]GG 932 CCAGG Cfr13I 156 GGACC G^G[ACGT]CC 439 GGGCC EarI 64 GAAGAG CTCTTC(1/4) 806 GAAGAG EcoRII 153 CCTGG ^CC[AT]GG 932 CCAGG Fnu4HI 287 GCTGC GC^[ACGT]GC 370 GCAGC HaeIII 354 GGCC GG^CC 440 GGCC ItaI 287 GCTGC GC^[ACGT]GC 370 GCAGC Ksp632I 64 GAAGAG CTCTTC(1/4) 806 GAAGAG MaeII 476 ACGT A^CGT 515 ACGT MseI 683 TTAA T^TAA 778 TTAA MvaI 153 CCTGG CC^[AT]GG 932 CCAGG Sau96I 156 GGACC G^G[ACGT]CC 439 GGGCC ScrFI 153 CCTGG CC^[ACGT]GG 932 CCAGG TfiI 843 GATTC G^A[AT]TC 855 GAATC Tru9I 683 TTAA T^TAA 778 TTAA TseI 287 GCTGC G^C[AT]GC 370 GCAGC TspRI 435 CACTG CA[GC]TG[ACGT][ACGT]^ 567 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI BstF5I DdeI DpnII FokI HinfI HphI MaeIII MboI MboII MnlI MslI NdeII NlaIII PleI Sau3AI Tsp45I Tsp509I