(Linear) (Six-base) MAPSORT of: KIEE5920.seq  Check: 866  from: 1  to: 930



KIEE5920.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:14  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     724     801     930 
   Size:        724      77     129

  Fragments arranged by size: 

                 724     129      77
 
ApoI r'AATT_y

Cuts at:      0     249     930 
   Size:        249     681
 
BamHI G'GATC_C

Cuts at:      0     677     930 
   Size:        677     253
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      24     930 
   Size:         24     906
 
BclI T'GATC_A

Cuts at:      0     134     930 
   Size:        134     796
 
Bpu10I CC'TnA_GC

Cuts at:      0     187     930 
   Size:        187     743
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     761     930 
   Size:        761     169
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     210     930 
   Size:        210     720
 
BseYI C'CCAG_C

Cuts at:      0     429     930 
   Size:        429     501
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     724     801     930 
   Size:        724      77     129

  Fragments arranged by size: 

                 724     129      77
 
BstEII G'GTnAC_C

Cuts at:      0      24     930 
   Size:         24     906
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     429     930 
   Size:        429     501
 
BstYI r'GATC_y

Cuts at:      0     268     677     930 
   Size:        268     409     253

  Fragments arranged by size: 

                 409     268     253
 
Bsu36I CC'TnA_GG

Cuts at:      0     236     673     930 
   Size:        236     437     257

  Fragments arranged by size: 

                 437     257     236
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     779     930 
   Size:        779     151
 
EarI CTCTTCn'nnn_

Cuts at:      0     100     620     902     930 
   Size:        100     520     282      28

  Fragments arranged by size: 

                 520     282     100      28
 
EcoRV GAT'ATC

Cuts at:      0     473     930 
   Size:        473     457
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      94     930 
   Size:         94     836
 
FspI TGC'GCA

Cuts at:      0     720     930 
   Size:        720     210
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     775     807     930 
   Size:        775      32     123

  Fragments arranged by size: 

                 775     123      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     294     446     930 
   Size:        294     152     484

  Fragments arranged by size: 

                 484     294     152
 
MslI CAynn'nnrTG

Cuts at:      0     172     583     844     930 
   Size:        172     411     261      86

  Fragments arranged by size: 

                 411     261     172      86
 
NdeI CA'TA_TG

Cuts at:      0     444     930 
   Size:        444     486
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     732     930 
   Size:        732     198
 
SapI GCTCTTCn'nnn_

Cuts at:      0     902     930 
   Size:        902      28
 
SfcI C'TryA_G

Cuts at:      0     864     930 
   Size:        864      66
 
StuI AGG'CCT

Cuts at:      0     706     930 
   Size:        706     224
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     471     930 
   Size:        471     459
 
TatI w'GTAC_w

Cuts at:      0     176     302     930 
   Size:        176     126     628

  Fragments arranged by size: 

                 628     176     126
 
XmnI GAAnn'nnTTC

Cuts at:      0     153     706     930 
   Size:        153     553     224

  Fragments arranged by size: 

                 553     224     153
 

 Enzymes that do cut:

     AarI     ApoI    BamHI     BbsI     BclI   Bpu10I     BsaI    BseRI
    BseYI    BspMI   BstEII    BstXI    BstYI   Bsu36I    BtgZI     EarI
    EcoRV     FalI     FspI    Hin4I     MmeI     MslI     NdeI    PflMI
     SapI     SfcI     StuI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I     BtgI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FseI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI