################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaBI BsaHI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NgoMIV NheI NlaIV NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 418 GTCTAC GT^[AC][GT]AC AflIII 718 ACGTGT A^C[GA][CT]GT AgeI 362 ACCGGT A^CCGGT AocI 873 CCTCAGG CC^T[ACGT]AGG AvaI 127 CTCGGG C^[CT]CG[GA]G BbrPI 717 CACGTG CAC^GTG BclI 401 TGATCA T^GATCA BcnI 437 CCGGG CC^[GC]GG BfaI 785 CTAG C^TAG BpmI 61 CTGGAG CTGGAG(16/14) BsaAI 717 CACGTG [CT]AC^GT[GA] BsaI 780 GAGACC GGTCTC(1/5) BsaWI 362 ACCGGT [AT]^CCGG[AT] BseRI 645 CTCCTC GAGGAG(10/8) BsmAI 780 GAGAC GTCTC(1/5) BsoBI 127 CTCGGG C^[CT]CG[GA]G BspDI 151 ATCGAT AT^CGAT BspMI 760 ACCTGC ACCTGC(4/8) BsrFI 362 ACCGGT [GA]^CCGG[CT] BsrI 117 ACTGG ACTGG(1/-1) Bsu36I 873 CCTCAGG CC^T[ACGT]AGG Cfr10I 362 ACCGGT [GA]^CCGG[CT] ClaI 151 ATCGAT AT^CGAT DraII 925 AGGACCT [GA]G^G[ACGT]CC[CT] DrdI 508 GACTCCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 371 CGGCCA [CT]^GGCC[GA] Eco81I 873 CCTCAGG CC^T[ACGT]AGG EcoNI 801 CCTGAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 925 AGGACCT [GA]G^G[ACGT]CC[CT] FauI 384 CCCGC CCCGC(4/6) FbaI 401 TGATCA T^GATCA HincII 472 GTCAAC GT[CT]^[GA]AC HindII 472 GTCAAC GT[CT]^[GA]AC MaeI 785 CTAG C^TAG MspA1I 385 CCGCTG C[AC]G^C[GT]G NciI 437 CCGGG CC^[GC]GG NdeI 375 CATATG CA^TATG PinAI 362 ACCGGT A^CCGGT PmaCI 717 CACGTG CAC^GTG PmlI 717 CACGTG CAC^GTG PpuMI 925 AGGACCT [GA]G^G[AT]CC[CT] PstI 449 CTGCAG CTGCA^G ScaI 114 AGTACT AGT^ACT SspI 616 AATATT AAT^ATT TaqI 152 TCGA T^CGA TthHB8I 152 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 385 CCGC CCGC(-3/-1) 370 GCGG AfaI 115 GTAC GT^AC 303 GTAC AlwI 135 GATCC GGATC(4/5) 149 GGATC AvaII 413 GGACC G^G[AT]CC 926 GGACC BsaJI 317 CCCTGG C^C[ACGT][ACGT]GG 437 CCGGGG BsmFI 625 GGGAC GGGAC(10/14) 542 GTCCC BsmI 331 GAATGC GAATGC(1/-1) 684 GCATTC Cfr13I 413 GGACC G^G[ACGT]CC 926 GGACC EarI 664 CTCTTC CTCTTC(1/4) 930 CTCTTC EcoT22I 333 ATGCAT ATGCA^T 682 ATGCAT HaeIII 354 GGCC GG^CC 372 GGCC HapII 363 CCGG C^CGG 437 CCGG HpaII 363 CCGG C^CGG 437 CCGG Ksp632I 664 CTCTTC CTCTTC(1/4) 930 CTCTTC MseI 142 TTAA T^TAA 349 TTAA MslI 168 CACACCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 834 CACAGTGGTG MspI 363 CCGG C^CGG 437 CCGG NsiI 333 ATGCAT ATGCA^T 682 ATGCAT PleI 508 GACTC GAGTC(4/5) 627 GACTC RsaI 115 GTAC GT^AC 303 GTAC Sau96I 413 GGACC G^G[ACGT]CC 926 GGACC Tru9I 142 TTAA T^TAA 349 TTAA Tsp45I 506 GTGAC ^GT[GC]AC 536 GTGAC TspRI 23 CACTG CA[GC]TG[ACGT][ACGT]^ 836 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AluI BbvI BsiYI BslI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII Sau3AI ScrFI SfaNI SfcI TfiI TseI Tsp509I