################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI Aor51HI ApaLI AscI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI ItaI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TseI Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 335 GTCTAC GT^[AC][GT]AC AcsI 920 AAATTT [GA]^AATT[CT] AflIII 404 ACATGT A^C[GA][CT]GT AluI 708 AGCT AG^CT AlwNI 323 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 873 CCTGAGG CC^T[ACGT]AGG ApaI 232 GGGCCC GGGCC^C ApoI 920 AAATTT [GA]^AATT[CT] AspHI 290 GTGCTC G[AT]GC[AT]^C BanII 232 GGGCCC G[GA]GC[CT]^C BbsI 307 GTCTTC GAAGAC(2/6) BbvCI 691 GCTGAGG CCTCAGC(-5/-2) BglII 249 AGATCT A^GATCT BpuAI 307 GTCTTC GAAGAC(2/6) BsaJI 753 CCAGGG C^C[ACGT][ACGT]GG BsiHKAI 290 GTGCTC G[AT]GC[AT]^C BspLU11I 404 ACATGT A^CATGT BssKI 753 CCAGG ^CC[ACGT]GG BstAPI 413 GCAGAGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 753 CCAGG CC^[AT]GG BstXI 236 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 249 AGATCT [GA]^GATC[CT] Bsu36I 873 CCTGAGG CC^T[ACGT]AGG DraII 896 AGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 781 CGGCCA [CT]^GGCC[GA] Eco81I 873 CCTGAGG CC^T[ACGT]AGG EcoO109I 896 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRII 753 CCAGG ^CC[AT]GG EcoRV 538 GATATC GAT^ATC HapII 780 CCGG C^CGG HincII 400 GTCAAC GT[CT]^[GA]AC HindII 400 GTCAAC GT[CT]^[GA]AC HinfI 325 GATTC G^A[ACGT]TC HpaII 780 CCGG C^CGG HphI 599 TCACC GGTGA(8/7) MaeIII 73 GTGAC ^GT[ACGT]AC MflI 249 AGATCT [GA]^GATC[CT] MspI 780 CCGG C^CGG MvaI 753 CCAGG CC^[AT]GG NdeI 475 CATATG CA^TATG ScrFI 753 CCAGG CC^[ACGT]GG TfiI 325 GATTC G^A[AT]TC Tsp45I 73 GTGAC ^GT[GC]AC XbaI 801 TCTAGA T^CTAGA XhoII 249 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 492 CCGC CCGC(-3/-1) 739 GCGG AfaI 366 GTAC GT^AC 626 GTAC AseI 109 ATTAAT AT^TAAT 520 ATTAAT AsnI 109 ATTAAT AT^TAAT 520 ATTAAT BfaI 410 CTAG C^TAG 802 CTAG BmyI 232 GGGCCC G[AGT]GC[ACT]^C 290 GTGCTC BseRI 18 CTCCTC GAGGAG(10/8) 545 CTCCTC BsiYI 277 CCCTTCTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 356 CCTTTGATCGG BslI 277 CCCTTCTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 356 CCTTTGATCGG Bsp1286I 232 GGGCCC G[AGT]GC[ACT]^C 290 GTGCTC BstF5I 663 CATCC GGATG(2/0) 885 GGATG FokI 663 CATCC GGATG(9/13) 885 GGATG MaeI 410 CTAG C^TAG 802 CTAG MslI 168 CACACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 834 CACCCTTGTG MwoI 234 GCCCAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 413 GCAGAGTGTGC NlaIV 232 GGGCCC GG[ACGT]^[ACGT]CC 843 GGTTCC NspI 404 ACATGT [GA]CATG^[CT] 718 ACATGC PshBI 109 ATTAAT AT^TAAT 520 ATTAAT RsaI 366 GTAC GT^AC 626 GTAC SfaNI 240 GATGC GCATC(5/9) 463 GATGC TspRI 347 CAGTG CA[GC]TG[ACGT][ACGT]^ 482 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### BsrI Cfr13I DdeI DpnII HaeIII MboI MboII MnlI MseI NdeII NlaIII Sau3AI Sau96I Tru9I Tsp509I