################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvaII AviII BanI BbeI BbrPI BcgI BciVI BcnI BfrI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BsrBI BsrDI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) AcsI 923 AAATTC [GA]^AATT[CT] AflIII 316 ACATGT A^C[GA][CT]GT AlwNI 412 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 923 AAATTC [GA]^AATT[CT] AspI 402 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 282 CCTAGG C^CTAGG BamHI 494 GGATCC G^GATCC BanII 915 GGGCTC G[GA]GC[CT]^C BbsI 334 GTCTTC GAAGAC(2/6) BbvCI 186 CCTCAGC CCTCAGC(-5/-2) BclI 635 TGATCA T^GATCA BfaI 283 CTAG C^TAG BglI 346 GCCGTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 508 AGATCT A^GATCT BlnI 282 CCTAGG C^CTAGG BmyI 915 GGGCTC G[AGT]GC[ACT]^C BpuAI 334 GTCTTC GAAGAC(2/6) BseRI 219 CTCCTC GAGGAG(10/8) Bsp1286I 915 GGGCTC G[AGT]GC[ACT]^C Bsp1407I 392 TGTACA T^GTACA BspHI 632 TCATGA T^CATGA BspLU11I 316 ACATGT A^CATGT BspMI 653 ACCTGC ACCTGC(4/8) BsrGI 392 TGTACA T^GTACA BssKI 600 CCTGG ^CC[ACGT]GG BstEII 399 GGTGACC G^GT[ACGT]ACC BstNI 600 CCTGG CC^[AT]GG BstPI 399 GGTGACC G^GT[ACGT]ACC DsaI 753 CCATGG C^C[GA][CT]GG EcoO65I 399 GGTGACC G^GT[ACGT]ACC EcoRII 600 CCTGG ^CC[AT]GG FbaI 635 TGATCA T^GATCA HincII 808 GTTGAC GT[CT]^[GA]AC HindII 808 GTTGAC GT[CT]^[GA]AC HindIII 707 AAGCTT A^AGCTT MaeI 283 CTAG C^TAG MaeII 112 ACGT A^CGT MvaI 600 CCTGG CC^[AT]GG NcoI 753 CCATGG C^CATGG NspI 316 ACATGT [GA]CATG^[CT] PflFI 402 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 689 CTGCAG CTGCA^G RcaI 632 TCATGA T^CATGA ScrFI 600 CCTGG CC^[ACGT]GG SfiI 345 GGCCGTGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 392 TGTACA T^GTACA Tth111I 402 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AfaI 177 GTAC GT^AC 393 GTAC AlwI 495 GATCC GGATC(4/5) 494 GGATC BbvI 417 GCTGC GCAGC(8/12) 440 GCTGC BsgI 15 CTGCAC GTGCAG(16/14) 137 CTGCAC BsmFI 454 GGGAC GGGAC(10/14) 58 GTCCC BstF5I 639 CATCC GGATG(2/0) 885 GGATG BstYI 494 GGATCC [GA]^GATC[CT] 508 AGATCT EarI 79 CTCTTC CTCTTC(1/4) 499 CTCTTC Fnu4HI 417 GCTGC GC^[ACGT]GC 440 GCTGC FokI 639 CATCC GGATG(9/13) 885 GGATG ItaI 417 GCTGC GC^[ACGT]GC 440 GCTGC Ksp632I 79 CTCTTC CTCTTC(1/4) 499 CTCTTC MflI 494 GGATCC [GA]^GATC[CT] 508 AGATCT MseI 257 TTAA T^TAA 521 TTAA MwoI 346 GCCGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 420 GCGTGTGGAGC RsaI 177 GTAC GT^AC 393 GTAC Tru9I 257 TTAA T^TAA 521 TTAA TseI 417 GCTGC G^C[AT]GC 440 GCTGC XhoII 494 GGATCC [GA]^GATC[CT] 508 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AluI BalI BsaJI BsiYI BslI BsmAI Cac8I DdeI DpnII EaeI EcoT14I HaeIII HinfI HphI MaeIII MboI MboII MluNI MnlI MscI MslI NdeII NlaIII NlaIV PleI Sau3AI SfcI StyI TfiI Tsp45I Tsp509I TspRI