Restriction map for
pFN21AE5934
(Linear) (Six-base) MAPSORT of: KIEE5934.seq Check: 6237 from: 1 to: 933
KIEE5934.orf
With 182 enzymes: SgfI *
December 5, 2014 13:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 933
Size: 697 236
AflIII A'CryG_T
Cuts at: 0 316 933
Size: 316 617
AleI CACnn'nnGTG
Cuts at: 0 22 172 838 933
Size: 22 150 666 95
Fragments arranged by size:
666 150 95 22
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 447 479 933
Size: 447 32 454
Fragments arranged by size:
454 447 32
AlwNI CAG_nnn'CTG
Cuts at: 0 417 933
Size: 417 516
ApoI r'AATT_y
Cuts at: 0 923 933
Size: 923 10
AvrII C'CTAG_G
Cuts at: 0 282 933
Size: 282 651
BamHI G'GATC_C
Cuts at: 0 494 933
Size: 494 439
BanII G_rGCy'C
Cuts at: 0 919 933
Size: 919 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 327 933
Size: 327 606
BbvCI CC'TCA_GC
Cuts at: 0 187 933
Size: 187 746
BclI T'GATC_A
Cuts at: 0 635 933
Size: 635 298
BglI GCCn_nnn'nGGC
Cuts at: 0 352 933
Size: 352 581
BglII A'GATC_T
Cuts at: 0 508 933
Size: 508 425
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 94 450 933
Size: 94 356 483
Fragments arranged by size:
483 356 94
Bpu10I CC'TnA_GC
Cuts at: 0 187 483 933
Size: 187 296 450
Fragments arranged by size:
450 296 187
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 533 563 933
Size: 533 30 370
Fragments arranged by size:
533 370 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 210 933
Size: 210 723
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 933
Size: 122 811
Bsp1286I G_dGCh'C
Cuts at: 0 919 933
Size: 919 14
BspHI T'CATG_A
Cuts at: 0 632 933
Size: 632 301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 662 933
Size: 662 271
BsrGI T'GTAC_A
Cuts at: 0 392 933
Size: 392 541
BstEII G'GTnAC_C
Cuts at: 0 399 933
Size: 399 534
BstYI r'GATC_y
Cuts at: 0 494 508 933
Size: 494 14 425
Fragments arranged by size:
494 425 14
BtgI C'CryG_G
Cuts at: 0 753 933
Size: 753 180
BtsI GCAGTG_nn'
Cuts at: 0 589 933
Size: 589 344
EaeI y'GGCC_r
Cuts at: 0 344 371 605 818 933
Size: 344 27 234 213 115
Fragments arranged by size:
344 234 213 115 27
EarI CTCTTCn'nnn_
Cuts at: 0 85 505 933
Size: 85 420 428
Fragments arranged by size:
428 420 85
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 933
Size: 697 236
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 578 933
Size: 578 355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 393 425 933
Size: 393 32 508
Fragments arranged by size:
508 393 32
HincII GTy'rAC
Cuts at: 0 810 933
Size: 810 123
HindIII A'AGCT_T
Cuts at: 0 707 933
Size: 707 226
MscI TGG'CCA
Cuts at: 0 373 607 820 933
Size: 373 234 213 113
Fragments arranged by size:
373 234 213 113
MslI CAynn'nnrTG
Cuts at: 0 22 172 838 933
Size: 22 150 666 95
Fragments arranged by size:
666 150 95 22
NcoI C'CATG_G
Cuts at: 0 753 933
Size: 753 180
NspI r_CATG'y
Cuts at: 0 320 933
Size: 320 613
PciI A'CATG_T
Cuts at: 0 316 933
Size: 316 617
PstI C_TGCA'G
Cuts at: 0 693 933
Size: 693 240
SfcI C'TryA_G
Cuts at: 0 504 689 867 933
Size: 504 185 178 66
Fragments arranged by size:
504 185 178 66
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 352 933
Size: 352 581
StyI C'CwwG_G
Cuts at: 0 42 282 753 933
Size: 42 240 471 180
Fragments arranged by size:
471 240 180 42
TatI w'GTAC_w
Cuts at: 0 176 392 933
Size: 176 216 541
Fragments arranged by size:
541 216 176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 581 613 933
Size: 581 32 320
Fragments arranged by size:
581 320 32
Tth111I GACn'n_nGTC
Cuts at: 0 405 933
Size: 405 528
Enzymes that do cut:
AcuI AflIII AleI AloI AlwNI ApoI AvrII BamHI
BanII BbsI BbvCI BclI BglI BglII BplI Bpu10I
BsaXI BseRI BsgI Bsp1286I BspHI BspMI BsrGI BstEII
BstYI BtgI BtsI EaeI EarI Eco57MI FalI Hin4I
HincII HindIII MscI MslI NcoI NspI PciI PstI
SfcI SfiI StyI TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
BaeI BanI BbeI BcgI BciVI BfrBI BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MmeI MspA1I NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
XbaI XcmI XhoI XmaI XmnI ZraI