Restriction map for
pFN21AE5456
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII BanI BbeI BbrPI
BcgI BciVI BcnI BpmI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoT22I FauI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MfeI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 363 CCGC CCGC(-3/-1)
AcsI 923 AAATTC [GA]^AATT[CT]
AfaI 177 GTAC GT^AC
AflII 552 CTTAAG C^TTAAG
ApoI 923 AAATTC [GA]^AATT[CT]
AvrII 282 CCTAGG C^CTAGG
BamHI 494 GGATCC G^GATCC
BbvCI 186 CCTCAGC CCTCAGC(-5/-2)
BbvI 440 GCTGC GCAGC(8/12)
BclI 635 TGATCA T^GATCA
BfaI 283 CTAG C^TAG
BfrI 552 CTTAAG C^TTAAG
BglI 346 GCCGTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 508 AGATCT A^GATCT
BlnI 282 CCTAGG C^CTAGG
BlpI 482 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 482 GCTTAGC GC^T[ACGT]AGC
BseRI 219 CTCCTC GAGGAG(10/8)
BsgI 15 CTGCAC GTGCAG(16/14)
BsmAI 586 GAGAC GTCTC(1/5)
BspHI 632 TCATGA T^CATGA
BspMI 653 ACCTGC ACCTGC(4/8)
BsrI 785 CCAGT ACTGG(1/-1)
CelII 482 GCTTAGC GC^T[ACGT]AGC
Cfr13I 782 GGCCC G^G[ACGT]CC
DsaI 753 CCATGG C^C[GA][CT]GG
EarI 79 CTCTTC CTCTTC(1/4)
EcoRV 571 GATATC GAT^ATC
FbaI 635 TGATCA T^GATCA
Fnu4HI 440 GCTGC GC^[ACGT]GC
ItaI 440 GCTGC GC^[ACGT]GC
Ksp632I 79 CTCTTC CTCTTC(1/4)
MaeI 283 CTAG C^TAG
MaeII 112 ACGT A^CGT
NcoI 753 CCATGG C^CATGG
PflMI 404 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 689 CTGCAG CTGCA^G
RcaI 632 TCATGA T^CATGA
RsaI 177 GTAC GT^AC
Sau96I 782 GGCCC G^G[ACGT]CC
SfaNI 805 GATGC GCATC(5/9)
SfiI 345 GGCCGTGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 552 CTTAAG C^T[CT][GA]AG
TseI 440 GCTGC G^C[AT]GC
Van91I 404 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 671 CCATCCTGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AlwI 495 GATCC GGATC(4/5)
494 GGATC
BanII 684 GGGCTC G[GA]GC[CT]^C
915 GGGCTC
BbsI 334 GTCTTC GAAGAC(2/6)
748 GTCTTC
BmyI 684 GGGCTC G[AGT]GC[ACT]^C
915 GGGCTC
BpuAI 334 GTCTTC GAAGAC(2/6)
748 GTCTTC
BsmFI 454 GGGAC GGGAC(10/14)
58 GTCCC
Bsp1286I 684 GGGCTC G[AGT]GC[ACT]^C
915 GGGCTC
BssKI 126 CCTGG ^CC[ACGT]GG
600 CCTGG
BstF5I 639 CATCC GGATG(2/0)
672 CATCC
BstNI 126 CCTGG CC^[AT]GG
600 CCTGG
BstYI 494 GGATCC [GA]^GATC[CT]
508 AGATCT
EcoRII 126 CCTGG ^CC[AT]GG
600 CCTGG
FokI 639 CATCC GGATG(9/13)
672 CATCC
MflI 494 GGATCC [GA]^GATC[CT]
508 AGATCT
MvaI 126 CCTGG CC^[AT]GG
600 CCTGG
MwoI 346 GCCGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
420 GCGTGTGGAGC
PleI 71 GAGTC GAGTC(4/5)
107 GAGTC
ScrFI 126 CCTGG CC^[ACGT]GG
600 CCTGG
XhoII 494 GGATCC [GA]^GATC[CT]
508 AGATCT
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI BalI BsaJI BsiYI BslI
Cac8I DdeI DpnII EaeI EcoT14I
HaeIII HinfI HphI MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI NdeII NlaIII NlaIV Sau3AI
SfcI StyI TfiI Tru9I Tsp45I
Tsp509I TspRI