################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcsI AflII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FauI FbaI FokI FseI FspI HaeIII HapII HgaI HincII HindII HindIII HpaI HpaII KpnI KspI MaeII MfeI MflI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvnI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcyI 704 GGCGCC G[GA]^CG[CT]C AfaI 183 GTAC GT^AC AflIII 902 ACATGT A^C[GA][CT]GT AluI 422 AGCT AG^CT AlwNI 113 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 879 CCTTAGG CC^T[ACGT]AGG Asp700I 711 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 446 GGTCC G^G[AT]CC BanI 704 GGCGCC G^G[CT][GA]CC BbeI 704 GGCGCC GGCGC^C BcgI 848 CGATTCCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 806 CTAG C^TAG BsaBI 141 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 704 GGCGCC G[GA]^CG[CT]C BsaJI 759 CCATGG C^C[ACGT][ACGT]GG BsmAI 414 GTCTC GTCTC(1/5) BspLU11I 902 ACATGT A^CATGT BssKI 75 CCAGG ^CC[ACGT]GG Bst1107I 399 GTATAC GTA^TAC BstNI 75 CCAGG CC^[AT]GG BstZ17I 399 GTATAC GTA^TAC Bsu36I 879 CCTTAGG CC^T[ACGT]AGG Cac8I 316 GCTTGC GC[ACGT]^[ACGT]GC CfoI 705 GCGC GCG^C Cfr13I 446 GGTCC G^G[ACGT]CC DraII 445 GGGTCCT [GA]G^G[ACGT]CC[CT] DsaI 759 CCATGG C^C[GA][CT]GG EarI 431 CTCTTC CTCTTC(1/4) Eco81I 879 CCTTAGG CC^T[ACGT]AGG EcoO109I 445 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRII 75 CCAGG ^CC[AT]GG EcoRV 892 GATATC GAT^ATC EcoT14I 759 CCATGG C^C[AT][AT]GG HaeII 704 GGCGCC [GA]GCGC^[CT] HhaI 705 GCGC GCG^C Hin1I 704 GGCGCC G[GA]^CG[CT]C HinP1I 705 GCGC G^CGC KasI 704 GGCGCC G^GCGCC Ksp632I 431 CTCTTC CTCTTC(1/4) MaeI 806 CTAG C^TAG MamI 141 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvaI 75 CCAGG CC^[AT]GG MwoI 352 GCAGCGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 704 GGCGCC GG^CGCC NcoI 759 CCATGG C^CATGG NspI 902 ACATGT [GA]CATG^[CT] PleI 155 GACTC GAGTC(4/5) PpuMI 445 GGGTCCT [GA]G^G[AT]CC[CT] RsaI 183 GTAC GT^AC SapI 430 GCTCTTC GCTCTTC(1/4) Sau96I 446 GGTCC G^G[ACGT]CC ScrFI 75 CCAGG CC^[ACGT]GG SfoI 704 GGCGCC GGC^GCC StyI 759 CCATGG C^C[AT][AT]GG XmnI 711 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AccI 106 GTCTAC GT^[AC][GT]AC 399 GTATAC AspHI 428 GTGCTC G[AT]GC[AT]^C 468 GTGCTC BmyI 428 GTGCTC G[AGT]GC[ACT]^C 468 GTGCTC BsiHKAI 428 GTGCTC G[AT]GC[AT]^C 468 GTGCTC BsiYI 369 CCGTTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 449 CCTATACATGG BslI 369 CCGTTTTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 449 CCTATACATGG Bsp1286I 428 GTGCTC G[AGT]GC[ACT]^C 468 GTGCTC DpnII 692 GATC ^GATC 870 GATC HphI 627 GGTGA GGTGA(8/7) 923 TCACC MboI 692 GATC ^GATC 870 GATC MslI 117 CACTGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 405 CACTATTATG NdeII 692 GATC ^GATC 870 GATC Sau3AI 692 GATC ^GATC 870 GATC TfiI 514 GAATC G^A[AT]TC 849 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### BbvI DdeI Fnu4HI HinfI ItaI MaeIII MboII MnlI NlaIII NlaIV SfaNI SfcI TseI Tsp45I Tsp509I TspRI