################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmFI BsoBI Bsp1286I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HhaI Hin1I HinP1I HinfI HpaII HphI KasI KpnI Ksp632I KspI MaeII MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI TaqI TfiI Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AciI 357 CCGC CCGC(-3/-1) AfaI 765 GTAC GT^AC AflII 157 CTTAAG C^TTAAG AflIII 709 ACATGT A^C[GA][CT]GT AvaII 748 GGACC G^G[AT]CC BbsI 258 GAAGAC GAAGAC(2/6) BfaI 230 CTAG C^TAG BfrI 157 CTTAAG C^TTAAG BglII 326 AGATCT A^GATCT BpmI 322 CTGGAG CTGGAG(16/14) BpuAI 258 GAAGAC GAAGAC(2/6) BsaBI 327 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 91 CGTCTC CGTCTC(1/5) Bsp1407I 764 TGTACA T^GTACA BspLU11I 709 ACATGT A^CATGT BsrGI 764 TGTACA T^GTACA BsrI 405 CCAGT ACTGG(1/-1) BstXI 306 CCATGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 791 CCATGG C^C[GA][CT]GG EcoT14I 791 CCATGG C^C[AT][AT]GG HgaI 90 GCGTC GACGC(5/10) HindIII 892 AAGCTT A^AGCTT HpaI 577 GTTAAC GTT^AAC MaeI 230 CTAG C^TAG MamI 327 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MwoI 607 GCCATTTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 791 CCATGG C^CATGG PflMI 493 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 765 GTAC GT^AC SfaNI 244 GATGC GCATC(5/9) SmlI 157 CTTAAG C^T[CT][GA]AG SspBI 764 TGTACA T^GTACA StyI 791 CCATGG C^C[AT][AT]GG Van91I 493 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 405 CCAGTGGGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AluI 807 AGCT AG^CT 893 AGCT BamHI 51 GGATCC G^GATCC 445 GGATCC BbvI 537 GCAGC GCAGC(8/12) 317 GCTGC BclI 512 TGATCA T^GATCA 911 TGATCA BsaJI 437 CCTGGG C^C[ACGT][ACGT]GG 791 CCATGG BsgI 382 CTGCAC GTGCAG(16/14) 614 GTGCAG BsmAI 92 GTCTC GTCTC(1/5) 715 GTCTC BsmI 456 GCATTC GAATGC(1/-1) 660 GCATTC FbaI 512 TGATCA T^GATCA 911 TGATCA Fnu4HI 317 GCTGC GC^[ACGT]GC 537 GCAGC HincII 352 GTTGAC GT[CT]^[GA]AC 577 GTTAAC HindII 352 GTTGAC GT[CT]^[GA]AC 577 GTTAAC ItaI 317 GCTGC GC^[ACGT]GC 537 GCAGC MaeIII 19 GTGAC ^GT[ACGT]AC 530 GTGAC MseI 158 TTAA T^TAA 578 TTAA MslI 723 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 756 CATATTTATG NspI 709 ACATGT [GA]CATG^[CT] 769 ACATGC SspI 69 AATATT AAT^ATT 636 AATATT Tru9I 158 TTAA T^TAA 578 TTAA TseI 317 GCTGC G^C[AT]GC 537 GCAGC Tsp45I 19 GTGAC ^GT[GC]AC 530 GTGAC Tsp509I 118 AATT ^AATT 908 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AlwI BsiYI BslI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII FokI HaeIII MboI MboII MflI MnlI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TspRI XhoII