Restriction map for
pFN21AE5585
##################################
# NO CUT ENZYMES ( 174 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfaI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FauI FseI FspI HapII HgaI
Hin1I HincII HindII HpaI HpaII
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI MwoI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AciI 970 GCGG CCGC(-3/-1)
BalI 530 TGGCCA TGG^CCA
BclI 755 TGATCA T^GATCA
BpmI 712 CTGGAG CTGGAG(16/14)
BseRI 1053 GAGGAG GAGGAG(10/8)
BsgI 723 CTGCAC GTGCAG(16/14)
BspHI 563 TCATGA T^CATGA
BsrI 711 ACTGG ACTGG(1/-1)
BstYI 880 GGATCT [GA]^GATC[CT]
CfoI 855 GCGC GCG^C
DraII 611 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 509 CCATGG C^C[GA][CT]GG
EaeI 530 TGGCCA [CT]^GGCC[GA]
EcoO109I 611 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 918 ATGCAT ATGCA^T
FbaI 755 TGATCA T^GATCA
HaeII 854 GGCGCT [GA]GCGC^[CT]
HhaI 855 GCGC GCG^C
HinP1I 855 GCGC G^CGC
HindIII 174 AAGCTT A^AGCTT
MflI 880 GGATCT [GA]^GATC[CT]
MluNI 530 TGGCCA TGG^CCA
MscI 530 TGGCCA TGG^CCA
NcoI 509 CCATGG C^CATGG
NsiI 918 ATGCAT ATGCA^T
PleI 409 GACTC GAGTC(4/5)
RcaI 563 TCATGA T^CATGA
SapI 316 GCTCTTC GCTCTTC(1/4)
TfiI 213 GAATC G^A[AT]TC
XhoII 880 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 256 GTCTAC GT^[AC][GT]AC
406 GTAGAC
AcsI 92 AAATTT [GA]^AATT[CT]
635 AAATTC
AlwI 126 GATCC GGATC(4/5)
880 GGATC
ApoI 92 AAATTT [GA]^AATT[CT]
635 AAATTC
BsmAI 327 GTCTC GTCTC(1/5)
805 GTCTC
BsmFI 201 GGGAC GGGAC(10/14)
913 GTCCC
BsmI 584 GCATTC GAATGC(1/-1)
621 GCATTC
BsrDI 261 CATTGC GCAATG(2/0)
888 CATTGC
BstF5I 43 GGATG GGATG(2/0)
366 GGATG
Cfr13I 612 GGGCC G^G[ACGT]CC
667 GGCCC
EarI 317 CTCTTC CTCTTC(1/4)
555 CTCTTC
EcoT14I 152 CCTTGG C^C[AT][AT]GG
509 CCATGG
FokI 43 GGATG GGATG(9/13)
366 GGATG
HinfI 213 GAATC G^A[ACGT]TC
409 GACTC
Ksp632I 317 CTCTTC CTCTTC(1/4)
555 CTCTTC
NlaIV 611 GGGGCC GG[ACGT]^[ACGT]CC
667 GGCCCC
NspI 161 ACATGC [GA]CATG^[CT]
281 ACATGC
Sau96I 612 GGGCC G^G[ACGT]CC
667 GGCCC
SspI 975 AATATT AAT^ATT
1099 AATATT
StyI 152 CCTTGG C^C[AT][AT]GG
509 CCATGG
Tsp45I 421 GTGAC ^GT[GC]AC
695 GTGAC
TspRI 726 CACTG CA[GC]TG[ACGT][ACGT]^
765 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI BbvI BsaJI BsiYI
BslI BssKI BstNI Cac8I DdeI
DpnII EcoRII Fnu4HI HaeIII HphI
ItaI MaeIII MboI MboII MnlI
MseI MslI MvaI NdeII NlaIII
RsaI Sau3AI ScrFI SfaNI Tru9I
TseI Tsp509I