################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT14I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AlwI 471 GATCC GGATC(4/5) AocI 666 CCTAAGG CC^T[ACGT]AGG BalI 365 TGGCCA TGG^CCA BbsI 715 GTCTTC GAAGAC(2/6) BclI 236 TGATCA T^GATCA BpuAI 715 GTCTTC GAAGAC(2/6) BsaJI 179 CCCTGG C^C[ACGT][ACGT]GG BsmFI 162 GTCCC GGGAC(10/14) BsmI 864 GAATGC GAATGC(1/-1) Bsp1407I 764 TGTACA T^GTACA BspHI 398 TCATGA T^CATGA BspMI 774 ACCTGC ACCTGC(4/8) BsrDI 588 CATTGC GCAATG(2/0) BsrGI 764 TGTACA T^GTACA BssKI 180 CCTGG ^CC[ACGT]GG BstEII 132 GGTCACC G^GT[ACGT]ACC BstNI 180 CCTGG CC^[AT]GG BstPI 132 GGTCACC G^GT[ACGT]ACC BstYI 470 AGATCC [GA]^GATC[CT] Bsu36I 666 CCTAAGG CC^T[ACGT]AGG DdeI 667 CTAAG C^T[ACGT]AG DraII 160 GGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 358 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 365 TGGCCA [CT]^GGCC[GA] Eco81I 666 CCTAAGG CC^T[ACGT]AGG EcoO109I 160 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 132 GGTCACC G^GT[ACGT]ACC EcoRII 180 CCTGG ^CC[AT]GG EcoT22I 282 ATGCAT ATGCA^T FbaI 236 TGATCA T^GATCA MflI 470 AGATCC [GA]^GATC[CT] MluNI 365 TGGCCA TGG^CCA MscI 365 TGGCCA TGG^CCA MvaI 180 CCTGG CC^[AT]GG NdeI 571 CATATG CA^TATG NsiI 282 ATGCAT ATGCA^T PpuMI 160 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 288 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 776 CTGCAG CTGCA^G RcaI 398 TCATGA T^CATGA ScrFI 180 CCTGG CC^[ACGT]GG SfcI 776 CTGCAG C^T[GA][CT]AG SspBI 764 TGTACA T^GTACA XcmI 146 CCAGCCCATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 470 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AfaI 171 GTAC GT^AC 765 GTAC AluI 679 AGCT AG^CT 780 AGCT AspHI 121 GTGCTC G[AT]GC[AT]^C 660 GTGCTC AvaII 161 GGTCC G^G[AT]CC 502 GGTCC BbvI 778 GCAGC GCAGC(8/12) 592 GCTGC BsaI 771 GAGACC GGTCTC(1/5) 639 GGTCTC BsgI 558 CTGCAC GTGCAG(16/14) 320 GTGCAG BsiHKAI 121 GTGCTC G[AT]GC[AT]^C 660 GTGCTC BsmAI 771 GAGAC GTCTC(1/5) 640 GTCTC BsrI 156 ACTGG ACTGG(1/-1) 405 CCAGT Cfr13I 161 GGTCC G^G[ACGT]CC 502 GGTCC EarI 475 CTCTTC CTCTTC(1/4) 583 CTCTTC Fnu4HI 592 GCTGC GC^[ACGT]GC 778 GCAGC HphI 919 GGTGA GGTGA(8/7) 134 TCACC ItaI 592 GCTGC GC^[ACGT]GC 778 GCAGC Ksp632I 475 CTCTTC CTCTTC(1/4) 583 CTCTTC MaeIII 133 GTCAC ^GT[ACGT]AC 920 GTGAC NlaIV 160 GGGTCC GG[ACGT]^[ACGT]CC 161 GGTCCC PflMI 357 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 570 CCATATGCTGG PleI 288 GACTC GAGTC(4/5) 672 GACTC RsaI 171 GTAC GT^AC 765 GTAC Sau96I 161 GGTCC G^G[ACGT]CC 502 GGTCC SmlI 290 CTCAAG C^T[CT][GA]AG 858 CTTGAG TfiI 51 GAATC G^A[AT]TC 605 GATTC TseI 592 GCTGC G^C[AT]GC 778 GCAGC Tsp45I 133 GTCAC ^GT[GC]AC 920 GTGAC Tsp509I 488 AATT ^AATT 893 AATT Van91I 357 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 570 CCATATGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### BmyI BsiYI BslI Bsp1286I BstF5I Cac8I DpnII FokI HaeIII HinfI MboI MboII MnlI MseI MslI NdeII NlaIII Sau3AI SfaNI Tru9I TspRI