################################## # NO CUT ENZYMES ( 177 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MluI MroI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AlwNI 803 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 121 GTGCTC G[AT]GC[AT]^C AvaII 502 GGTCC G^G[AT]CC BalI 365 TGGCCA TGG^CCA BmyI 121 GTGCTC G[AGT]GC[ACT]^C BpmI 776 CTCCAG CTGGAG(16/14) BsgI 558 CTGCAC GTGCAG(16/14) BsiHKAI 121 GTGCTC G[AT]GC[AT]^C BsmI 864 GAATGC GAATGC(1/-1) Bsp1286I 121 GTGCTC G[AGT]GC[ACT]^C Bsp1407I 764 TGTACA T^GTACA BspHI 398 TCATGA T^CATGA BsrDI 588 CATTGC GCAATG(2/0) BsrGI 764 TGTACA T^GTACA BstEII 132 GGTCACC G^GT[ACGT]ACC BstPI 132 GGTCACC G^GT[ACGT]ACC Cfr13I 502 GGTCC G^G[ACGT]CC DraIII 358 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 365 TGGCCA [CT]^GGCC[GA] EcoO65I 132 GGTCACC G^GT[ACGT]ACC HphI 134 TCACC GGTGA(8/7) MluNI 365 TGGCCA TGG^CCA MscI 365 TGGCCA TGG^CCA NdeI 609 CATATG CA^TATG NspI 408 GCATGT [GA]CATG^[CT] PflMI 357 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 288 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 398 TCATGA T^CATGA Sau96I 502 GGTCC G^G[ACGT]CC SfaNI 202 GATGC GCATC(5/9) SspBI 764 TGTACA T^GTACA Van91I 357 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwI 161 GATCC GGATC(4/5) 471 GATCC BbvI 405 GCAGC GCAGC(8/12) 592 GCTGC BclI 236 TGATCA T^GATCA 545 TGATCA BfaI 424 CTAG C^TAG 577 CTAG BsaI 771 GAGACC GGTCTC(1/5) 639 GGTCTC BssKI 180 CCTGG ^CC[ACGT]GG 636 CCTGG BstF5I 330 CATCC GGATG(2/0) 280 GGATG BstNI 180 CCTGG CC^[AT]GG 636 CCTGG BstYI 160 AGATCC [GA]^GATC[CT] 470 AGATCC EarI 475 CTCTTC CTCTTC(1/4) 583 CTCTTC EcoRII 180 CCTGG ^CC[AT]GG 636 CCTGG FbaI 236 TGATCA T^GATCA 545 TGATCA Fnu4HI 405 GCAGC GC^[ACGT]GC 592 GCTGC FokI 330 CATCC GGATG(9/13) 280 GGATG ItaI 405 GCAGC GC^[ACGT]GC 592 GCTGC Ksp632I 475 CTCTTC CTCTTC(1/4) 583 CTCTTC MaeI 424 CTAG C^TAG 577 CTAG MaeIII 19 GTGAC ^GT[ACGT]AC 133 GTCAC MflI 160 AGATCC [GA]^GATC[CT] 470 AGATCC MslI 393 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 756 CATATTTGTG MvaI 180 CCTGG CC^[AT]GG 636 CCTGG PleI 288 GACTC GAGTC(4/5) 581 GACTC ScrFI 180 CCTGG CC^[ACGT]GG 636 CCTGG SmlI 290 CTCAAG C^T[CT][GA]AG 858 CTTGAG TfiI 51 GAATC G^A[AT]TC 244 GATTC TseI 405 GCAGC G^C[AT]GC 592 GCTGC Tsp45I 19 GTGAC ^GT[GC]AC 133 GTCAC Tsp509I 488 AATT ^AATT 893 AATT XhoII 160 AGATCC [GA]^GATC[CT] 470 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AfaI AluI BsiYI BslI BsmAI Cac8I DdeI DpnII HaeIII HinfI MboI MboII MnlI MseI NdeII NlaIII RsaI Sau3AI Tru9I TspRI