################################## # NO CUT ENZYMES ( 163 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MflI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 864 AAATTC [GA]^AATT[CT] AflIII 533 ACATGT A^C[GA][CT]GT ApoI 864 AAATTC [GA]^AATT[CT] AspHI 304 GAGCAC G[AT]GC[AT]^C AspI 25 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 782 GGACC G^G[AT]CC BbsI 736 GTCTTC GAAGAC(2/6) BbvI 423 GCTGC GCAGC(8/12) BciVI 537 GTATCC GTATCC(6/5) BclI 516 TGATCA T^GATCA BpmI 897 CTGGAG CTGGAG(16/14) BpuAI 736 GTCTTC GAAGAC(2/6) BsiHKAI 304 GAGCAC G[AT]GC[AT]^C BspHI 398 TCATGA T^CATGA BspLU11I 533 ACATGT A^CATGT BstF5I 218 CATCC GGATG(2/0) BstXI 570 CCATGCTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DpnII 517 GATC ^GATC DraI 666 TTTAAA TTT^AAA DraII 781 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 107 CCATGG C^C[GA][CT]GG EcoO109I 781 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 202 GATATC GAT^ATC FbaI 516 TGATCA T^GATCA Fnu4HI 423 GCTGC GC^[ACGT]GC FokI 218 CATCC GGATG(9/13) HinfI 51 GAATC G^A[ACGT]TC ItaI 423 GCTGC GC^[ACGT]GC MboI 517 GATC ^GATC MwoI 769 GCTAGACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 107 CCATGG C^CATGG NdeII 517 GATC ^GATC NspI 533 ACATGT [GA]CATG^[CT] PflFI 25 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 493 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 781 AGGACCT [GA]G^G[AT]CC[CT] PstI 711 CTGCAG CTGCA^G RcaI 398 TCATGA T^CATGA SapI 90 GCTCTTC GCTCTTC(1/4) Sau3AI 517 GATC ^GATC ScaI 69 AGTACT AGT^ACT SfcI 711 CTGCAG C^T[GA][CT]AG TaqI 302 TCGA T^CGA TfiI 51 GAATC G^A[AT]TC TseI 423 GCTGC G^C[AT]GC Tth111I 25 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 302 TCGA T^CGA Van91I 493 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 596 CCAATGGAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AluI 293 AGCT AG^CT 893 AGCT BalI 365 TGGCCA TGG^CCA 593 TGGCCA BanII 159 GGGCTC G[GA]GC[CT]^C 487 GGGCTC BfaI 770 CTAG C^TAG 779 CTAG BsaI 151 GAGACC GGTCTC(1/5) 905 GAGACC BsgI 454 CTGCAC GTGCAG(16/14) 558 CTGCAC BsiYI 493 CCATTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 582 CCTCTTAGTGG BslI 493 CCATTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 582 CCTCTTAGTGG BsrI 552 ACTGG ACTGG(1/-1) 606 ACTGG BssKI 156 CCTGG ^CC[ACGT]GG 437 CCTGG BstNI 156 CCTGG CC^[AT]GG 437 CCTGG Cac8I 184 GCTGGC GC[ACGT]^[ACGT]GC 610 GCTTGC EaeI 365 TGGCCA [CT]^GGCC[GA] 593 TGGCCA EarI 91 CTCTTC CTCTTC(1/4) 902 GAAGAG EcoRII 156 CCTGG ^CC[AT]GG 437 CCTGG EcoT14I 7 CCAAGG C^C[AT][AT]GG 107 CCATGG HphI 342 GGTGA GGTGA(8/7) 163 TCACC Ksp632I 91 CTCTTC CTCTTC(1/4) 902 GAAGAG MaeI 770 CTAG C^TAG 779 CTAG MluNI 365 TGGCCA TGG^CCA 593 TGGCCA MscI 365 TGGCCA TGG^CCA 593 TGGCCA MseI 667 TTAA T^TAA 847 TTAA MvaI 156 CCTGG CC^[AT]GG 437 CCTGG ScrFI 156 CCTGG CC^[ACGT]GG 437 CCTGG StyI 7 CCAAGG C^C[AT][AT]GG 107 CCATGG Tru9I 667 TTAA T^TAA 847 TTAA Tsp509I 262 AATT ^AATT 865 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AfaI BmyI BsaJI BsmAI Bsp1286I BspMI Cfr13I DdeI HaeIII MaeIII MboII MnlI MslI NlaIII NlaIV RsaI Sau96I Tsp45I TspRI