Restriction map for
pFN21AE5462
(Linear) (Six-base) MAPSORT of: KIEE5462.seq Check: 6087 from: 1 to: 927
KIEE5462.orf
With 182 enzymes: SgfI *
December 5, 2014 13:01 ..
AflIII A'CryG_T
Cuts at: 0 533 927
Size: 533 394
AleI CACnn'nnGTG
Cuts at: 0 619 927
Size: 619 308
ApoI r'AATT_y
Cuts at: 0 864 927
Size: 864 63
BanII G_rGCy'C
Cuts at: 0 163 491 927
Size: 163 328 436
Fragments arranged by size:
436 328 163
BbsI GAAGACnn'nnnn_
Cuts at: 0 729 927
Size: 729 198
BciVI GTATCCnnnnn_n'
Cuts at: 0 548 927
Size: 548 379
BclI T'GATC_A
Cuts at: 0 516 927
Size: 516 411
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 918 927
Size: 918 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 145 899 927
Size: 145 754 28
Fragments arranged by size:
754 145 28
BseYI C'CCAG_C
Cuts at: 0 290 927
Size: 290 637
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 543 927
Size: 439 104 384
Fragments arranged by size:
439 384 104
BsiHKAI G_wGCw'C
Cuts at: 0 308 927
Size: 308 619
Bsp1286I G_dGCh'C
Cuts at: 0 163 308 491 927
Size: 163 145 183 436
Fragments arranged by size:
436 183 163 145
BspHI T'CATG_A
Cuts at: 0 398 927
Size: 398 529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 718 783 927
Size: 128 590 65 144
Fragments arranged by size:
590 144 128 65
BstXI CCAn_nnnn'nTGG
Cuts at: 0 577 927
Size: 577 350
BtgI C'CryG_G
Cuts at: 0 107 927
Size: 107 820
DraI TTT'AAA
Cuts at: 0 668 927
Size: 668 259
EaeI y'GGCC_r
Cuts at: 0 365 593 927
Size: 365 228 334
Fragments arranged by size:
365 334 228
EarI CTCTTCn'nnn_
Cuts at: 0 97 897 927
Size: 97 800 30
Fragments arranged by size:
800 97 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 918 927
Size: 918 9
EcoO109I rG'GnC_Cy
Cuts at: 0 782 927
Size: 782 145
EcoRV GAT'ATC
Cuts at: 0 204 927
Size: 204 723
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 898 927
Size: 898 29
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 927
Size: 423 504
MscI TGG'CCA
Cuts at: 0 367 595 927
Size: 367 228 332
Fragments arranged by size:
367 332 228
MslI CAynn'nnrTG
Cuts at: 0 130 619 727 927
Size: 130 489 108 200
Fragments arranged by size:
489 200 130 108
NcoI C'CATG_G
Cuts at: 0 107 927
Size: 107 820
NspI r_CATG'y
Cuts at: 0 537 927
Size: 537 390
PasI CC'CwG_GG
Cuts at: 0 156 927
Size: 156 771
PciI A'CATG_T
Cuts at: 0 533 927
Size: 533 394
PflMI CCAn_nnn'nTGG
Cuts at: 0 499 927
Size: 499 428
PpuMI rG'GwC_Cy
Cuts at: 0 782 927
Size: 782 145
PstI C_TGCA'G
Cuts at: 0 715 927
Size: 715 212
SapI GCTCTTCn'nnn_
Cuts at: 0 97 927
Size: 97 830
ScaI AGT'ACT
Cuts at: 0 71 927
Size: 71 856
SfcI C'TryA_G
Cuts at: 0 711 927
Size: 711 216
StyI C'CwwG_G
Cuts at: 0 7 107 927
Size: 7 100 820
Fragments arranged by size:
820 100 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 399 583 927
Size: 399 184 344
Fragments arranged by size:
399 344 184
TatI w'GTAC_w
Cuts at: 0 69 170 922 927
Size: 69 101 752 5
Fragments arranged by size:
752 101 69 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 36 68 927
Size: 36 32 859
Fragments arranged by size:
859 36 32
Tth111I GACn'n_nGTC
Cuts at: 0 28 927
Size: 28 899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 603 927
Size: 603 324
Enzymes that do cut:
AflIII AleI ApoI BanII BbsI BciVI BclI BpmI
BsaI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BstXI
BtgI DraI EaeI EarI Eco57MI EcoO109I EcoRV Hin4I
MmeI MscI MslI NcoI NspI PasI PciI PflMI
PpuMI PstI SapI ScaI SfcI StyI TaqII TatI
TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI XhoI XmaI XmnI
ZraI