(Circular) (Six-base) MAPSORT of: pFN21AE3174.seq Check: 8116 from: 1 to: 5926 pFN21AE3174 5926 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3736 278 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3269 1937 Size: 1332 4594 Acc65I G'GTAC_C Cuts at: 2604 3247 2604 Size: 643 5283 AclI AA'CG_TT Cuts at: 1563 4053 4426 1563 Size: 2490 373 3063 Fragments arranged by size: 3063 2490 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2697 2933 3989 5373 5641 1632 Size: 1065 236 1056 1384 268 1917 Fragments arranged by size: 1917 1384 1065 1056 268 236 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5729 AflIII A'CryG_T Cuts at: 1203 4825 5802 1203 Size: 3622 977 1327 Fragments arranged by size: 3622 1327 977 AgeI A'CCGG_T Cuts at: 4757 4757 Size: 5926 AhdI GACnn_n'nnGTC Cuts at: 4655 4655 Size: 5926 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5926 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5926 AlwNI CAG_nnn'CTG Cuts at: 3669 5241 3669 Size: 1572 4354 ApaLI G'TGCA_C Cuts at: 3983 5139 3983 Size: 1156 4770 ApoI r'AATT_y Cuts at: 1493 3235 3461 3613 5722 1493 Size: 1742 226 152 2109 1697 Fragments arranged by size: 2109 1742 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2462 4480 160 Size: 872 1430 2018 1606 Fragments arranged by size: 2018 1606 1430 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 AvaI C'yCGr_G Cuts at: 1870 1943 2139 3251 1870 Size: 73 196 1112 4545 Fragments arranged by size: 4545 1112 196 73 BamHI G'GATC_C Cuts at: 3256 3623 3256 Size: 367 5559 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2604 3247 4702 618 Size: 325 217 627 817 643 1455 1842 Fragments arranged by size: 1842 1455 817 643 627 325 217 BanII G_rGCy'C Cuts at: 1391 2892 3118 3245 1391 Size: 1501 226 127 4072 Fragments arranged by size: 4072 1501 226 127 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2921 928 Size: 971 1022 3933 Fragments arranged by size: 3933 1022 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4117 4151 1903 Size: 34 50 34 2096 34 3678 Fragments arranged by size: 3678 2096 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2025 2418 3818 5028 2025 Size: 393 1400 1210 2923 Fragments arranged by size: 2923 1400 1210 393 BclI T'GATC_A Cuts at: 1548 1548 Size: 5926 BfrBI ATG'CAT Cuts at: 2381 2381 Size: 5926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4537 136 Size: 107 122 71 631 605 2865 1525 Fragments arranged by size: 2865 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2180 5921 2180 Size: 3741 2185 BlpI GC'TnA_GC Cuts at: 3196 3343 3196 Size: 147 5779 Bme1580I G_kGCm'C Cuts at: 1790 2310 3987 5143 1790 Size: 520 1677 1156 2573 Fragments arranged by size: 2573 1677 1156 520 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5926 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3657 4615 442 Size: 416 53 83 399 24 2240 958 1753 Fragments arranged by size: 2240 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5926 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3132 3132 Size: 5926 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2493 4586 1239 Size: 1254 2093 2579 Fragments arranged by size: 2579 2093 1254 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2145 2169 4051 4916 5214 2145 Size: 24 1882 865 298 2857 Fragments arranged by size: 2857 1882 865 298 24 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2358 2929 4589 882 Size: 617 859 571 1660 2219 Fragments arranged by size: 2219 1660 859 617 571 BsaAI yAC'GTr Cuts at: 493 1204 3717 493 Size: 711 2513 2702 Fragments arranged by size: 2702 2513 711 BsaBI GATnn'nnATC Cuts at: 5920 5920 Size: 5926 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3733 4115 4750 275 Size: 53 83 186 936 2200 382 635 1451 Fragments arranged by size: 2200 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4359 4757 5031 5178 5589 4359 Size: 398 274 147 411 4696 Fragments arranged by size: 4696 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5701 Fragments arranged by size: 5701 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2702 1186 Size: 1516 4410 BseYI C'CCAG_C Cuts at: 2431 2757 5129 5625 2431 Size: 326 2372 496 2732 Fragments arranged by size: 2732 2372 496 326 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2498 1163 Size: 573 762 4591 Fragments arranged by size: 4591 762 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3376 4137 5165 1540 Size: 275 186 1375 761 1028 2301 Fragments arranged by size: 2301 1375 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2892 3118 3987 4072 5143 2892 Size: 226 869 85 1071 3675 Fragments arranged by size: 3675 1071 869 226 85 BsmI GAATG_Cn' Cuts at: 2048 2644 3437 3530 2048 Size: 596 793 93 4444 Fragments arranged by size: 4444 793 596 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2310 2892 3118 3245 3987 4072 5143 Size: 399 520 582 226 127 742 85 1071 Cuts at: 5143 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 582 520 399 226 127 85 BspEI T'CCGG_A Cuts at: 5589 5589 Size: 5926 BspHI T'CATG_A Cuts at: 3815 5545 3815 Size: 1730 4196 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2592 3281 844 Size: 1748 689 3489 Fragments arranged by size: 3489 1748 689 BsrBI CCG'CTC Cuts at: 1394 1823 3813 1394 Size: 429 1990 3507 Fragments arranged by size: 3507 1990 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4421 4595 65 Size: 1183 3173 174 1396 Fragments arranged by size: 3173 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4570 4757 1700 Size: 110 2760 187 2869 Fragments arranged by size: 2869 2760 187 110 BsrGI T'GTAC_A Cuts at: 96 3172 96 Size: 3076 2850 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5926 BssSI C'ACGA_G Cuts at: 3986 4998 3986 Size: 1012 4914 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5926 BstBI TT'CG_AA Cuts at: 4730 4730 Size: 5926 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5926 BstYI r'GATC_y Cuts at: 1972 2180 2509 3256 3623 4008 4025 5477 5563 Size: 208 329 747 367 385 17 1452 86 Cuts at: 5563 5575 5921 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 747 385 367 346 329 208 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5374 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5187 Fragments arranged by size: 5187 683 56 BtsI GCAGTG_nn' Cuts at: 2619 3442 4236 4256 2619 Size: 823 794 20 4289 Fragments arranged by size: 4289 823 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5891 DraI TTT'AAA Cuts at: 3230 3230 Size: 5926 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2943 4933 809 Size: 507 1627 1990 1802 Fragments arranged by size: 1990 1802 1627 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2090 3373 4262 Size: 54 1425 50 275 18 260 1283 889 Cuts at: 4262 5891 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1283 889 275 260 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3373 1537 Size: 275 1561 4090 Fragments arranged by size: 4090 1561 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2871 3106 3856 1353 Size: 1518 235 750 3423 Fragments arranged by size: 3423 1518 750 235 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4513 4887 5033 387 Size: 1064 138 2924 374 146 1280 Fragments arranged by size: 2924 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2890 3116 2890 Size: 226 5700 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2493 2697 2933 3989 4586 5373 5641 Size: 393 861 204 236 1056 597 787 268 Cuts at: 5641 1239 Size: 1524 Fragments arranged by size: 1524 1056 861 787 597 393 268 236 204 EcoNI CCTnn'n_nnAGG Cuts at: 2079 2547 2079 Size: 468 5458 EcoRI G'AATT_C Cuts at: 3235 3613 3235 Size: 378 5548 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2063 3135 2063 Size: 1072 4854 FspI TGC'GCA Cuts at: 4432 4432 Size: 5926 HaeII r_GCGC'y Cuts at: 1678 3196 5073 1678 Size: 1518 1877 2531 Fragments arranged by size: 2531 1877 1518 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3201 3233 4567 4599 4641 Size: 32 135 32 1629 32 1334 32 42 Cuts at: 4641 4673 1373 Size: 32 2626 Fragments arranged by size: 2626 1629 1334 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3270 3516 669 Size: 988 281 1332 246 3079 Fragments arranged by size: 3079 1332 988 281 246 HindIII A'AGCT_T Cuts at: 748 3203 748 Size: 2455 3471 HpaI GTT'AAC Cuts at: 3516 3516 Size: 5926 KpnI G_GTAC'C Cuts at: 2608 3251 2608 Size: 643 5283 MfeI C'AATT_G Cuts at: 2718 3068 3525 2718 Size: 350 457 5119 Fragments arranged by size: 5119 457 350 MluI A'CGCG_T Cuts at: 5802 5802 Size: 5926 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2040 5040 5224 1305 Size: 735 3000 184 2007 Fragments arranged by size: 3000 2007 735 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2092 10 Size: 54 1425 343 260 3844 Fragments arranged by size: 3844 1425 343 260 54 MslI CAynn'nnrTG Cuts at: 518 1166 2588 2876 3884 4243 4402 518 Size: 648 1422 288 1008 359 159 2042 Fragments arranged by size: 2042 1422 1008 648 359 288 159 MspA1I CmG'CkG Cuts at: 1606 3342 4019 5167 5412 1606 Size: 1736 677 1148 245 2120 Fragments arranged by size: 2120 1736 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5374 NdeI CA'TA_TG Cuts at: 387 387 Size: 5926 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5926 NotI GC'GGCC_GC Cuts at: 3373 3373 Size: 5926 NsiI A_TGCA'T Cuts at: 2383 2383 Size: 5926 NspI r_CATG'y Cuts at: 1132 1229 2617 2699 3284 4829 1132 Size: 97 1388 82 585 1545 2229 Fragments arranged by size: 2229 1545 1388 585 97 82 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5926 PciI A'CATG_T Cuts at: 4825 4825 Size: 5926 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5926 PmeI GTTT'AAAC Cuts at: 3230 3230 Size: 5926 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3995 4027 5508 5540 3995 Size: 32 1481 32 4381 Fragments arranged by size: 4381 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5926 PsiI TTA'TAA Cuts at: 3496 3496 Size: 5926 PstI C_TGCA'G Cuts at: 830 2328 3278 830 Size: 1498 950 3478 Fragments arranged by size: 3478 1498 950 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5926 SacI G_AGCT'C Cuts at: 2892 3118 2892 Size: 226 5700 SalI G'TCGA_C Cuts at: 1936 3268 1936 Size: 1332 4594 SapI GCTCTTCn'nnn_ Cuts at: 2871 3106 2871 Size: 235 5691 SbfI CC_TGCA'GG Cuts at: 3278 3278 Size: 5926 ScaI AGT'ACT Cuts at: 4174 5886 4174 Size: 1712 4214 SexAI A'CCwGG_T Cuts at: 2938 2938 Size: 5926 SfcI C'TryA_G Cuts at: 826 1045 2109 2324 2825 3274 4409 5090 5281 Size: 219 1064 215 501 449 1135 681 191 Cuts at: 5281 826 Size: 1471 Fragments arranged by size: 1471 1135 1064 681 501 449 219 215 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5926 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5926 SmaI CCC'GGG Cuts at: 1872 2141 3253 1872 Size: 269 1112 4545 Fragments arranged by size: 4545 1112 269 SmlI C'TyrA_G Cuts at: 820 1017 1943 2160 2184 4030 4931 5193 820 Size: 197 926 217 24 1846 901 262 1553 Fragments arranged by size: 1846 1553 926 901 262 217 197 24 SnaBI TAC'GTA Cuts at: 493 3717 493 Size: 3224 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5926 SphI G_CATG'C Cuts at: 1132 2699 3284 1132 Size: 1567 585 3774 Fragments arranged by size: 3774 1567 585 SspI AAT'ATT Cuts at: 5 52 3850 5 Size: 47 3798 2081 Fragments arranged by size: 3798 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5926 StyI C'CwwG_G Cuts at: 513 1065 2355 3365 513 Size: 552 1290 1010 3074 Fragments arranged by size: 3074 1290 1010 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2505 3953 3970 4123 4308 4753 325 Size: 1255 925 1448 17 153 185 445 1498 Fragments arranged by size: 1498 1448 1255 925 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3172 4172 5884 Size: 274 80 33 51 1443 1195 1000 1712 Cuts at: 5884 96 Size: 138 Fragments arranged by size: 1712 1443 1195 1000 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2501 2533 5886 5918 2501 Size: 32 3353 32 2509 Fragments arranged by size: 3353 2509 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5926 XbaI T'CTAG_A Cuts at: 2281 3262 2281 Size: 981 4945 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5926 XmaI C'CCGG_G Cuts at: 1870 2139 3251 1870 Size: 269 1112 4545 Fragments arranged by size: 4545 1112 269 XmnI GAAnn'nnTTC Cuts at: 2244 4055 2244 Size: 1811 4115 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3734 276 Size: 53 83 186 936 2200 2468 Fragments arranged by size: 2468 2200 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI