(Circular) (Six-base) MAPSORT of: pFN21AE5809.seq Check: 8754 from: 1 to: 7444 pFN21AE5809 7444 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5254 278 Size: 53 83 186 936 3718 2468 Fragments arranged by size: 3718 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4787 1937 Size: 2850 4594 Acc65I G'GTAC_C Cuts at: 4765 4765 Size: 7444 AclI AA'CG_TT Cuts at: 1563 5571 5944 1563 Size: 4008 373 3063 Fragments arranged by size: 4008 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2457 3260 5507 6891 7159 1632 Size: 825 803 2247 1384 268 1917 Fragments arranged by size: 2247 1917 1384 825 803 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7247 AflIII A'CryG_T Cuts at: 1203 3107 3365 4525 6343 7320 1203 Size: 1904 258 1160 1818 977 1327 Fragments arranged by size: 1904 1818 1327 1160 977 258 AgeI A'CCGG_T Cuts at: 6275 6275 Size: 7444 AhdI GACnn_n'nnGTC Cuts at: 6173 6173 Size: 7444 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7444 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4506 1184 Size: 3322 4122 AlwNI CAG_nnn'CTG Cuts at: 2098 5187 6759 2098 Size: 3089 1572 2783 Fragments arranged by size: 3089 2783 1572 ApaLI G'TGCA_C Cuts at: 5501 6657 5501 Size: 1156 6288 ApoI r'AATT_y Cuts at: 1493 3177 3290 3592 3636 3703 4544 4685 4753 Size: 1684 113 302 44 67 841 141 68 Cuts at: 4753 4979 5131 7240 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1684 841 302 226 152 141 113 68 67 44 AseI AT'TA_AT Cuts at: 160 1032 4674 5998 160 Size: 872 3642 1324 1606 Fragments arranged by size: 3642 1606 1324 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7444 AvaI C'yCGr_G Cuts at: 1870 1943 4769 1870 Size: 73 2826 4545 Fragments arranged by size: 4545 2826 73 BamHI G'GATC_C Cuts at: 4774 5141 4774 Size: 367 7077 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4765 6220 618 Size: 325 217 627 2978 1455 1842 Fragments arranged by size: 2978 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2598 2839 4647 4763 1391 Size: 1207 241 1808 116 4072 Fragments arranged by size: 4072 1808 1207 241 116 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2364 928 Size: 971 465 6008 Fragments arranged by size: 6008 971 465 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5635 5669 1903 Size: 34 3698 34 3678 Fragments arranged by size: 3698 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2556 5336 6546 2556 Size: 2780 1210 3454 Fragments arranged by size: 3454 2780 1210 BclI T'GATC_A Cuts at: 1548 3054 3073 1548 Size: 1506 19 5919 Fragments arranged by size: 5919 1506 19 BfrBI ATG'CAT Cuts at: 3500 3500 Size: 7444 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6055 136 Size: 107 122 71 631 605 4383 1525 Fragments arranged by size: 4383 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7439 7439 Size: 7444 BlpI GC'TnA_GC Cuts at: 2968 4861 2968 Size: 1893 5551 Bme1580I G_kGCm'C Cuts at: 1790 3065 5505 6661 1790 Size: 1275 2440 1156 2573 Fragments arranged by size: 2573 2440 1275 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7444 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5175 6133 442 Size: 416 53 83 399 24 3758 958 1753 Fragments arranged by size: 3758 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7444 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3834 6104 1239 Size: 2595 2270 2579 Fragments arranged by size: 2595 2579 2270 Bpu10I CC'TnA_GC Cuts at: 2099 2487 2099 Size: 388 7056 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2363 2653 5569 6434 6732 2363 Size: 290 2916 865 298 3075 Fragments arranged by size: 3075 2916 865 298 290 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2105 3277 6107 882 Size: 617 606 1172 2830 2219 Fragments arranged by size: 2830 2219 1172 617 606 BsaAI yAC'GTr Cuts at: 493 1204 3108 3727 5235 493 Size: 711 1904 619 1508 2702 Fragments arranged by size: 2702 1904 1508 711 619 BsaBI GATnn'nnATC Cuts at: 7438 7438 Size: 7444 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3925 5251 5633 6268 Size: 53 83 186 936 2392 1326 382 635 Cuts at: 6268 275 Size: 1451 Fragments arranged by size: 2392 1451 1326 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5877 6275 6549 6696 7107 5877 Size: 398 274 147 411 6214 Fragments arranged by size: 6214 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3384 3414 1178 Size: 30 165 30 1981 30 5208 Fragments arranged by size: 5208 1981 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3522 4074 1186 Size: 2336 552 4556 Fragments arranged by size: 4556 2336 552 BseYI C'CCAG_C Cuts at: 6647 7143 6647 Size: 496 6948 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2553 4368 1163 Size: 573 817 1815 4239 Fragments arranged by size: 4239 1815 817 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4894 5655 6683 1540 Size: 275 186 2893 761 1028 2301 Fragments arranged by size: 2893 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2598 4647 5505 5590 6661 2598 Size: 2049 858 85 1071 3381 Fragments arranged by size: 3381 2049 1071 858 85 BsmI GAATG_Cn' Cuts at: 3328 4955 5048 3328 Size: 1627 93 5724 Fragments arranged by size: 5724 1627 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3916 3916 Size: 7444 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2598 2839 3065 4647 4763 5505 5590 Size: 399 808 241 226 1582 116 742 85 Cuts at: 5590 6661 1391 Size: 1071 2174 Fragments arranged by size: 2174 1582 1071 808 742 399 241 226 116 85 BspEI T'CCGG_A Cuts at: 7107 7107 Size: 7444 BspHI T'CATG_A Cuts at: 3979 4701 5333 7063 3979 Size: 722 632 1730 4360 Fragments arranged by size: 4360 1730 722 632 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3502 4407 4799 844 Size: 2658 905 392 3489 Fragments arranged by size: 3489 2658 905 392 BsrBI CCG'CTC Cuts at: 1394 1823 5331 1394 Size: 429 3508 3507 Fragments arranged by size: 3508 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5939 6113 65 Size: 1183 4691 174 1396 Fragments arranged by size: 4691 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6088 6275 1700 Size: 110 4278 187 2869 Fragments arranged by size: 4278 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7444 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7444 BssSI C'ACGA_G Cuts at: 5504 6516 5504 Size: 1012 6432 BstAPI GCAn_nnn'nTGC Cuts at: 794 4238 794 Size: 3444 4000 BstBI TT'CG_AA Cuts at: 6248 6248 Size: 7444 BstEII G'GTnAC_C Cuts at: 1184 3184 1184 Size: 2000 5444 BstYI r'GATC_y Cuts at: 1972 2017 2400 4774 5141 5526 5543 6995 7081 Size: 45 383 2374 367 385 17 1452 86 Cuts at: 7081 7093 7439 1972 Size: 12 346 1977 Fragments arranged by size: 2374 1977 1452 385 383 367 346 86 45 17 12 Bsu36I CC'TnA_GG Cuts at: 2168 2168 Size: 7444 BtgI C'CryG_G Cuts at: 513 1065 2004 3374 513 Size: 552 939 1370 4583 Fragments arranged by size: 4583 1370 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6705 Fragments arranged by size: 6705 683 56 BtsI GCAGTG_nn' Cuts at: 3333 4380 4960 5754 5774 3333 Size: 1047 580 794 20 5003 Fragments arranged by size: 5003 1047 794 580 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7444 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7409 DraI TTT'AAA Cuts at: 3739 4748 3739 Size: 1009 6435 DraIII CAC_nnn'GTG Cuts at: 2537 3071 2537 Size: 534 6910 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6451 809 Size: 507 5135 1802 Fragments arranged by size: 5135 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2767 4891 5780 Size: 54 1425 50 275 18 937 2124 889 Cuts at: 5780 7409 8 Size: 1629 43 Fragments arranged by size: 2124 1629 1425 937 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4891 1537 Size: 275 3079 4090 Fragments arranged by size: 4090 3079 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2603 3252 3795 4092 5374 1353 Size: 1250 649 543 297 1282 3423 Fragments arranged by size: 3423 1282 1250 649 543 297 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2051 2312 6031 6405 6551 387 Size: 1064 138 462 261 3719 374 146 1280 Fragments arranged by size: 3719 1280 1064 462 374 261 146 138 EcoICRI GAG'CTC Cuts at: 2596 4645 2596 Size: 2049 5395 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2457 3260 3834 5507 6104 6891 7159 Size: 393 825 803 574 1673 597 787 268 Cuts at: 7159 1239 Size: 1524 Fragments arranged by size: 1673 1524 825 803 787 597 574 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 4323 4323 Size: 7444 EcoO109I rG'GnC_Cy Cuts at: 3241 3241 Size: 7444 EcoRI G'AATT_C Cuts at: 4753 5131 4753 Size: 378 7066 EcoRV GAT'ATC Cuts at: 3612 4436 3612 Size: 824 6620 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2694 4220 4522 2694 Size: 1526 302 5616 Fragments arranged by size: 5616 1526 302 FspI TGC'GCA Cuts at: 5950 5950 Size: 7444 HaeII r_GCGC'y Cuts at: 1678 6591 1678 Size: 4913 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3031 3063 3131 3163 4392 Size: 32 135 32 1459 32 68 32 1229 Cuts at: 4392 4424 6085 6117 6159 6191 1373 Size: 32 1661 32 42 32 2626 Fragments arranged by size: 2626 1661 1459 1229 135 68 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3150 4788 5034 669 Size: 988 281 1212 1638 246 3079 Fragments arranged by size: 3079 1638 1212 988 281 246 HindIII A'AGCT_T Cuts at: 748 3520 748 Size: 2772 4672 HpaI GTT'AAC Cuts at: 5034 5034 Size: 7444 KpnI G_GTAC'C Cuts at: 4769 4769 Size: 7444 MfeI C'AATT_G Cuts at: 3842 5043 3842 Size: 1201 6243 MluI A'CGCG_T Cuts at: 3365 7320 3365 Size: 3955 3489 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2328 6558 6742 1305 Size: 1023 4230 184 2007 Fragments arranged by size: 4230 2007 1023 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2769 10 Size: 54 1425 343 937 4685 Fragments arranged by size: 4685 1425 937 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2939 3431 3629 3854 4517 5402 5761 Size: 648 1773 492 198 225 663 885 359 Cuts at: 5761 5920 518 Size: 159 2042 Fragments arranged by size: 2042 1773 885 663 648 492 359 225 198 159 MspA1I CmG'CkG Cuts at: 1606 2957 3280 4726 4860 5537 6685 6930 1606 Size: 1351 323 1446 134 677 1148 245 2120 Fragments arranged by size: 2120 1446 1351 1148 677 323 245 134 NcoI C'CATG_G Cuts at: 513 1065 2004 3374 513 Size: 552 939 1370 4583 Fragments arranged by size: 4583 1370 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7444 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7444 NotI GC'GGCC_GC Cuts at: 4891 4891 Size: 7444 NsiI A_TGCA'T Cuts at: 3502 3502 Size: 7444 NspI r_CATG'y Cuts at: 1132 1229 3355 4487 4529 4700 4802 6347 1132 Size: 97 2126 1132 42 171 102 1545 2229 Fragments arranged by size: 2229 2126 1545 1132 171 102 97 42 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7444 PciI A'CATG_T Cuts at: 4525 6343 4525 Size: 1818 5626 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7444 PfoI T'CCnGG_A Cuts at: 2022 3760 3811 2022 Size: 1738 51 5655 Fragments arranged by size: 5655 1738 51 PmeI GTTT'AAAC Cuts at: 4748 4748 Size: 7444 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5513 5545 7026 7058 5513 Size: 32 1481 32 5899 Fragments arranged by size: 5899 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3241 3241 Size: 7444 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7444 PsiI TTA'TAA Cuts at: 3854 5014 3854 Size: 1160 6284 PstI C_TGCA'G Cuts at: 830 4796 830 Size: 3966 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7444 PvuII CAG'CTG Cuts at: 2957 3280 4726 2957 Size: 323 1446 5675 Fragments arranged by size: 5675 1446 323 SacI G_AGCT'C Cuts at: 2598 4647 2598 Size: 2049 5395 SalI G'TCGA_C Cuts at: 1936 4786 1936 Size: 2850 4594 SapI GCTCTTCn'nnn_ Cuts at: 2603 2603 Size: 7444 SbfI CC_TGCA'GG Cuts at: 4796 4796 Size: 7444 ScaI AGT'ACT Cuts at: 4022 5692 7404 4022 Size: 1670 1712 4062 Fragments arranged by size: 4062 1712 1670 SexAI A'CCwGG_T Cuts at: 3243 3243 Size: 7444 SfcI C'TryA_G Cuts at: 826 1045 2958 4161 4792 5927 6608 6799 826 Size: 219 1913 1203 631 1135 681 191 1471 Fragments arranged by size: 1913 1471 1203 1135 681 631 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7444 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7444 SmaI CCC'GGG Cuts at: 1872 4771 1872 Size: 2899 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2342 2668 5548 6449 6711 820 Size: 197 926 399 326 2880 901 262 1553 Fragments arranged by size: 2880 1553 926 901 399 326 262 197 SnaBI TAC'GTA Cuts at: 493 3727 5235 493 Size: 3234 1508 2702 Fragments arranged by size: 3234 2702 1508 SpeI A'CTAG_T Cuts at: 152 152 Size: 7444 SphI G_CATG'C Cuts at: 1132 4802 1132 Size: 3670 3774 SspI AAT'ATT Cuts at: 5 52 2561 2774 3168 4108 4460 5368 5 Size: 47 2509 213 394 940 352 908 2081 Fragments arranged by size: 2509 2081 940 908 394 352 213 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7444 StyI C'CwwG_G Cuts at: 513 1065 2004 2411 3374 3903 4003 4883 513 Size: 552 939 407 963 529 100 880 3074 Fragments arranged by size: 3074 963 939 880 552 529 407 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5471 5488 5641 5826 6271 325 Size: 1255 3891 17 153 185 445 1498 Fragments arranged by size: 3891 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2655 4020 5690 Size: 274 80 33 51 1443 678 1365 1670 Cuts at: 5690 7402 96 Size: 1712 138 Fragments arranged by size: 1712 1670 1443 1365 678 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3355 3384 3387 3416 7404 7436 3355 Size: 29 3 29 3988 32 3363 Fragments arranged by size: 3988 3363 32 29 29 3 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7444 XbaI T'CTAG_A Cuts at: 4780 4780 Size: 7444 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3901 3901 Size: 7444 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7444 XmaI C'CCGG_G Cuts at: 1870 4769 1870 Size: 2899 4545 XmnI GAAnn'nnTTC Cuts at: 3683 4675 5573 3683 Size: 992 898 5554 Fragments arranged by size: 5554 992 898 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5252 276 Size: 53 83 186 936 3718 2468 Fragments arranged by size: 3718 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstXI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI