(Linear) (Six-base) MAPSORT of: KIEE5809.seq Check: 1797 from: 1 to: 2739 KIEE5809.orf With 182 enzymes: SgfI * December 5, 2014 13:11 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 452 1255 2739 Size: 452 803 1484 Fragments arranged by size: 1484 803 452 AflIII A'CryG_T Cuts at: 0 1102 1360 2520 2739 Size: 1102 258 1160 219 Fragments arranged by size: 1160 1102 258 219 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2501 2739 Size: 2501 238 AlwNI CAG_nnn'CTG Cuts at: 0 93 2739 Size: 93 2646 ApoI r'AATT_y Cuts at: 0 1172 1285 1587 1631 1698 2539 2680 2739 Size: 1172 113 302 44 67 841 141 59 Fragments arranged by size: 1172 841 302 141 113 67 59 44 AseI AT'TA_AT Cuts at: 0 2669 2739 Size: 2669 70 BanII G_rGCy'C Cuts at: 0 593 834 2642 2739 Size: 593 241 1808 97 Fragments arranged by size: 1808 593 241 97 BbsI GAAGACnn'nnnn_ Cuts at: 0 359 2739 Size: 359 2380 BciVI GTATCCnnnnn_n' Cuts at: 0 551 2739 Size: 551 2188 BclI T'GATC_A Cuts at: 0 1049 1068 2739 Size: 1049 19 1671 Fragments arranged by size: 1671 1049 19 BfrBI ATG'CAT Cuts at: 0 1495 2739 Size: 1495 1244 BlpI GC'TnA_GC Cuts at: 0 963 2739 Size: 963 1776 Bme1580I G_kGCm'C Cuts at: 0 1060 2739 Size: 1060 1679 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1829 2739 Size: 1829 910 Bpu10I CC'TnA_GC Cuts at: 0 94 482 2739 Size: 94 388 2257 Fragments arranged by size: 2257 388 94 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 358 648 2739 Size: 358 290 2091 Fragments arranged by size: 2091 358 290 BsaI GGTCTCn'nnnn_ Cuts at: 0 100 1272 2739 Size: 100 1172 1467 Fragments arranged by size: 1467 1172 100 BsaAI yAC'GTr Cuts at: 0 1103 1722 2739 Size: 1103 619 1017 Fragments arranged by size: 1103 1017 619 BsaHI Gr'CG_yC Cuts at: 0 1920 2739 Size: 1920 819 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1379 1409 2739 Size: 1379 30 1330 Fragments arranged by size: 1379 1330 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1517 2069 2739 Size: 1517 552 670 Fragments arranged by size: 1517 670 552 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 548 2363 2739 Size: 548 1815 376 Fragments arranged by size: 1815 548 376 BsiHKAI G_wGCw'C Cuts at: 0 593 2642 2739 Size: 593 2049 97 Fragments arranged by size: 2049 593 97 BsmI GAATG_Cn' Cuts at: 0 1323 2739 Size: 1323 1416 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1911 2739 Size: 1911 828 Bsp1286I G_dGCh'C Cuts at: 0 593 834 1060 2642 2739 Size: 593 241 226 1582 97 Fragments arranged by size: 1582 593 241 226 97 BspHI T'CATG_A Cuts at: 0 1974 2696 2739 Size: 1974 722 43 Fragments arranged by size: 1974 722 43 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1497 2402 2739 Size: 1497 905 337 Fragments arranged by size: 1497 905 337 BstAPI GCAn_nnn'nTGC Cuts at: 0 2233 2739 Size: 2233 506 BstEII G'GTnAC_C Cuts at: 0 1179 2739 Size: 1179 1560 BstYI r'GATC_y Cuts at: 0 12 395 2739 Size: 12 383 2344 Fragments arranged by size: 2344 383 12 Bsu36I CC'TnA_GG Cuts at: 0 163 2739 Size: 163 2576 BtgI C'CryG_G Cuts at: 0 1369 2739 Size: 1369 1370 BtsI GCAGTG_nn' Cuts at: 0 1328 2375 2739 Size: 1328 1047 364 Fragments arranged by size: 1328 1047 364 DraI TTT'AAA Cuts at: 0 1734 2739 Size: 1734 1005 DraIII CAC_nnn'GTG Cuts at: 0 532 1066 2739 Size: 532 534 1673 Fragments arranged by size: 1673 534 532 EaeI y'GGCC_r Cuts at: 0 762 2739 Size: 762 1977 EarI CTCTTCn'nnn_ Cuts at: 0 598 1247 1790 2087 2739 Size: 598 649 543 297 652 Fragments arranged by size: 652 649 598 543 297 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 46 307 2739 Size: 46 261 2432 Fragments arranged by size: 2432 261 46 EcoICRI GAG'CTC Cuts at: 0 591 2640 2739 Size: 591 2049 99 Fragments arranged by size: 2049 591 99 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 452 1255 1829 2739 Size: 452 803 574 910 Fragments arranged by size: 910 803 574 452 EcoNI CCTnn'n_nnAGG Cuts at: 0 2318 2739 Size: 2318 421 EcoO109I rG'GnC_Cy Cuts at: 0 1236 2739 Size: 1236 1503 EcoRV GAT'ATC Cuts at: 0 1607 2431 2739 Size: 1607 824 308 Fragments arranged by size: 1607 824 308 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 689 2215 2517 2739 Size: 689 1526 302 222 Fragments arranged by size: 1526 689 302 222 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1026 1058 1126 1158 2387 2419 2739 Size: 1026 32 68 32 1229 32 320 Fragments arranged by size: 1229 1026 320 68 32 32 32 HincII GTy'rAC Cuts at: 0 1145 2739 Size: 1145 1594 HindIII A'AGCT_T Cuts at: 0 1515 2739 Size: 1515 1224 MfeI C'AATT_G Cuts at: 0 1837 2739 Size: 1837 902 MluI A'CGCG_T Cuts at: 0 1360 2739 Size: 1360 1379 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 323 2739 Size: 323 2416 MscI TGG'CCA Cuts at: 0 764 2739 Size: 764 1975 MslI CAynn'nnrTG Cuts at: 0 934 1426 1624 1849 2512 2739 Size: 934 492 198 225 663 227 Fragments arranged by size: 934 663 492 227 225 198 MspA1I CmG'CkG Cuts at: 0 952 1275 2721 2739 Size: 952 323 1446 18 Fragments arranged by size: 1446 952 323 18 NcoI C'CATG_G Cuts at: 0 1369 2739 Size: 1369 1370 NsiI A_TGCA'T Cuts at: 0 1497 2739 Size: 1497 1242 NspI r_CATG'y Cuts at: 0 1350 2482 2524 2695 2739 Size: 1350 1132 42 171 44 Fragments arranged by size: 1350 1132 171 44 42 PciI A'CATG_T Cuts at: 0 2520 2739 Size: 2520 219 PfoI T'CCnGG_A Cuts at: 0 17 1755 1806 2739 Size: 17 1738 51 933 Fragments arranged by size: 1738 933 51 17 PpuMI rG'GwC_Cy Cuts at: 0 1236 2739 Size: 1236 1503 PsiI TTA'TAA Cuts at: 0 1849 2739 Size: 1849 890 PvuII CAG'CTG Cuts at: 0 952 1275 2721 2739 Size: 952 323 1446 18 Fragments arranged by size: 1446 952 323 18 SacI G_AGCT'C Cuts at: 0 593 2642 2739 Size: 593 2049 97 Fragments arranged by size: 2049 593 97 SapI GCTCTTCn'nnn_ Cuts at: 0 598 2739 Size: 598 2141 ScaI AGT'ACT Cuts at: 0 2017 2739 Size: 2017 722 SexAI A'CCwGG_T Cuts at: 0 1238 2739 Size: 1238 1501 SfcI C'TryA_G Cuts at: 0 953 2156 2739 Size: 953 1203 583 Fragments arranged by size: 1203 953 583 SmlI C'TyrA_G Cuts at: 0 337 663 2739 Size: 337 326 2076 Fragments arranged by size: 2076 337 326 SnaBI TAC'GTA Cuts at: 0 1722 2739 Size: 1722 1017 SspI AAT'ATT Cuts at: 0 556 769 1163 2103 2455 2739 Size: 556 213 394 940 352 284 Fragments arranged by size: 940 556 394 352 284 213 StyI C'CwwG_G Cuts at: 0 406 1369 1898 1998 2739 Size: 406 963 529 100 741 Fragments arranged by size: 963 741 529 406 100 TatI w'GTAC_w Cuts at: 0 650 2015 2739 Size: 650 1365 724 Fragments arranged by size: 1365 724 650 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1350 1379 1382 1411 2739 Size: 1350 29 3 29 1328 Fragments arranged by size: 1350 1328 29 29 3 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1896 2739 Size: 1896 843 XmnI GAAnn'nnTTC Cuts at: 0 1678 2670 2739 Size: 1678 992 69 Fragments arranged by size: 1678 992 69 Enzymes that do cut: AcuI AflIII AlfI AlwNI ApoI AseI BanII BbsI BciVI BclI BfrBI BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BstAPI BstEII BstYI Bsu36I BtgI BtsI DraI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII HindIII MfeI MluI MmeI MscI MslI MspA1I NcoI NsiI NspI PciI PfoI PpuMI PsiI PvuII SacI SapI ScaI SexAI SfcI SmlI SnaBI SspI StyI TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BglI BglII BmgBI BmrI BmtI BplI BsaBI BsaWI BseYI BsiEI BsiWI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I BtgZI ClaI CspCI DrdI EagI EcoRI FseI FspI FspAI HaeII HpaI KasI KpnI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PasI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI StuI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI