################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssSI Bst1107I BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI MaeIII MfeI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tsp509I TspRI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 35 GGTACC G^GTACC AflIII 875 ACGCGT A^C[GA][CT]GT Asp700I 591 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 35 GGTACC G^GTACC BbsI 559 GTCTTC GAAGAC(2/6) BbvCI 792 CCTCAGC CCTCAGC(-5/-2) BglII 451 AGATCT A^GATCT BpuAI 559 GTCTTC GAAGAC(2/6) BsaBI 132 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 571 GTGCAG GTGCAG(16/14) BsiEI 627 CGACCG CG[GA][CT]^CG BsmAI 310 GAGAC GTCTC(1/5) BsmBI 310 GAGACG CGTCTC(1/5) BsrI 78 CCAGT ACTGG(1/-1) BssHII 520 GCGCGC G^CGCGC BstAPI 756 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 424 TTCGAA TT^CGAA BstXI 110 CCAGCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EcoNI 117 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 716 CCAAGG C^C[AT][AT]GG HaeII 582 GGCGCT [GA]GCGC^[CT] KpnI 35 GGTACC GGTAC^C KspI 907 CCGCGG CCGC^GG MamI 132 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 875 ACGCGT A^CGCGT NruI 750 TCGCGA TCG^CGA NspV 424 TTCGAA TT^CGAA PaeR7I 945 CTCGAG C^TCGAG PflMI 858 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 664 GACTC GAGTC(4/5) SacII 907 CCGCGG CCGC^GG SfaNI 137 GCATC GCATC(5/9) SfuI 424 TTCGAA TT^CGAA SmlI 945 CTCGAG C^T[CT][GA]AG SphI 542 GCATGC GCATG^C StyI 716 CCAAGG C^C[AT][AT]GG TfiI 979 GATTC G^A[AT]TC Van91I 858 CCACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 945 CTCGAG C^TCGAG XmnI 591 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 36 GTAC GT^AC 612 GTAC AlwI 132 GATCC GGATC(4/5) 126 GGATC AlwNI 757 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG 892 CAGCACCTG BanI 35 GGTACC G^G[CT][GA]CC 483 GGTGCC BfaI 41 CTAG C^TAG 737 CTAG BstYI 126 GGATCT [GA]^GATC[CT] 451 AGATCT DdeI 793 CTCAG C^T[ACGT]AG 975 CTCAG DsaI 212 CCACGG C^C[GA][CT]GG 907 CCGCGG EarI 826 GAAGAG CTCTTC(1/4) 991 GAAGAG HinfI 664 GACTC G^A[ACGT]TC 979 GATTC Ksp632I 826 GAAGAG CTCTTC(1/4) 991 GAAGAG MaeI 41 CTAG C^TAG 737 CTAG MflI 126 GGATCT [GA]^GATC[CT] 451 AGATCT NspI 185 GCATGT [GA]CATG^[CT] 542 GCATGC RsaI 36 GTAC GT^AC 612 GTAC SfiI 207 GGCCCCCACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 805 GGCCAGAAGGGCC XhoII 126 GGATCT [GA]^GATC[CT] 451 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AluI ApaI AvaI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI Sau3AI Sau96I ScrFI SmaI TaqI TseI TthHB8I XcmI XmaCI XmaI