(Circular) (Six-base) MAPSORT of: pFN21AB7941.seq Check: 9411 from: 1 to: 5734 pFN21AB7941 5734 bp With 182 enzymes: SgfI * March 28, 2012 12:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2665 2910 2665 Size: 245 5489 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3544 278 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3077 1937 Size: 1140 4594 Acc65I G'GTAC_C Cuts at: 2040 3055 2040 Size: 1015 4719 AclI AA'CG_TT Cuts at: 1563 3861 4234 1563 Size: 2298 373 3063 Fragments arranged by size: 3063 2298 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2348 3797 5181 5449 1632 Size: 716 1449 1384 268 1917 Fragments arranged by size: 1917 1449 1384 716 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5537 AflIII A'CryG_T Cuts at: 1203 2880 4633 5610 1203 Size: 1677 1753 977 1327 Fragments arranged by size: 1753 1677 1327 977 AgeI A'CCGG_T Cuts at: 4565 4565 Size: 5734 AhdI GACnn_n'nnGTC Cuts at: 4463 4463 Size: 5734 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5734 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5734 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2585 2617 2585 Size: 32 5702 AlwNI CAG_nnn'CTG Cuts at: 2767 2902 3477 5049 2767 Size: 135 575 1572 3452 Fragments arranged by size: 3452 1572 575 135 ApaI G_GGCC'C Cuts at: 2215 2364 2822 2849 2215 Size: 149 458 27 5100 Fragments arranged by size: 5100 458 149 27 ApaLI G'TGCA_C Cuts at: 3791 4947 3791 Size: 1156 4578 ApoI r'AATT_y Cuts at: 1493 3043 3269 3421 5530 1493 Size: 1550 226 152 2109 1697 Fragments arranged by size: 2109 1697 1550 226 152 AseI AT'TA_AT Cuts at: 160 1032 4288 160 Size: 872 3256 1606 Fragments arranged by size: 3256 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 AvaI C'yCGr_G Cuts at: 1870 1943 2257 2356 2498 2848 2950 2960 3059 Size: 73 314 99 142 350 102 10 99 Cuts at: 3059 1870 Size: 4545 Fragments arranged by size: 4545 350 314 142 102 99 99 73 10 BamHI G'GATC_C Cuts at: 3064 3431 3064 Size: 367 5367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2040 2488 3055 4510 618 Size: 325 217 627 253 448 567 1455 1842 Fragments arranged by size: 1842 1455 627 567 448 325 253 217 BanII G_rGCy'C Cuts at: 1391 2215 2255 2364 2822 2849 3053 1391 Size: 824 40 109 458 27 204 4072 Fragments arranged by size: 4072 824 458 204 109 40 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2557 928 Size: 971 658 4105 Fragments arranged by size: 4105 971 658 BbvCI CC'TCA_GC Cuts at: 2798 2798 Size: 5734 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3925 3959 1903 Size: 34 1988 34 3678 Fragments arranged by size: 3678 1988 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3626 4836 3626 Size: 1210 4524 BclI T'GATC_A Cuts at: 1548 1548 Size: 5734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2203 2219 2277 Size: 107 122 71 631 605 531 16 58 Cuts at: 2277 2817 4345 136 Size: 540 1528 1525 Fragments arranged by size: 1528 1525 631 605 540 531 122 107 71 58 16 BglII A'GATC_T Cuts at: 2456 5729 2456 Size: 3273 2461 BlpI GC'TnA_GC Cuts at: 3151 3151 Size: 5734 Bme1580I G_kGCm'C Cuts at: 1790 2181 2215 2364 2493 2822 2849 3795 4951 Size: 391 34 149 129 329 27 946 1156 Cuts at: 4951 1790 Size: 2573 Fragments arranged by size: 2573 1156 946 391 329 149 129 34 27 BmgBI CAC'GTC Cuts at: 1318 2575 2806 1318 Size: 1257 231 4246 Fragments arranged by size: 4246 1257 231 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2077 3465 4423 Size: 416 53 83 399 24 660 1388 958 Cuts at: 4423 442 Size: 1753 Fragments arranged by size: 1753 1388 958 660 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5734 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2418 2418 Size: 5734 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2098 2375 2464 2612 4394 1239 Size: 859 277 89 148 1782 2579 Fragments arranged by size: 2579 1782 859 277 148 89 Bpu10I CC'TnA_GC Cuts at: 2798 2798 Size: 5734 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3859 4724 5022 3859 Size: 865 298 4571 Fragments arranged by size: 4571 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4397 882 Size: 617 2898 2219 Fragments arranged by size: 2898 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3525 493 Size: 711 2321 2702 Fragments arranged by size: 2702 2321 711 BsaBI GATnn'nnATC Cuts at: 2141 5728 2141 Size: 3587 2147 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3541 3923 4558 275 Size: 53 83 186 936 2008 382 635 1451 Fragments arranged by size: 2008 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4167 4565 4839 4986 5397 4167 Size: 398 274 147 411 4504 Fragments arranged by size: 4504 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2237 2267 2585 2615 2870 Size: 30 165 30 834 30 318 30 255 Cuts at: 2870 2900 1178 Size: 30 4012 Fragments arranged by size: 4012 834 318 255 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2012 2344 2849 2852 2855 3005 3014 1186 Size: 826 332 505 3 3 150 9 3906 Fragments arranged by size: 3906 826 505 332 150 9 3 3 BseYI C'CCAG_C Cuts at: 2380 2470 2656 2858 2895 4937 5433 2380 Size: 90 186 202 37 2042 496 2681 Fragments arranged by size: 2681 2042 496 202 186 90 37 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2597 1163 Size: 573 861 4300 Fragments arranged by size: 4300 861 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2635 3184 3945 4973 1540 Size: 275 186 634 549 761 1028 2301 Fragments arranged by size: 2301 1028 761 634 549 275 186 BsiHKAI G_wGCw'C Cuts at: 3795 3880 4951 3795 Size: 85 1071 4578 Fragments arranged by size: 4578 1071 85 BsmI GAATG_Cn' Cuts at: 3245 3338 3245 Size: 93 5641 BsmBI CGTCTCn'nnnn_ Cuts at: 2309 2309 Size: 5734 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2215 2255 2364 2493 2822 2849 Size: 399 391 34 40 109 129 329 27 Cuts at: 2849 3053 3795 3880 4951 1391 Size: 204 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 391 329 204 129 109 85 40 34 27 BspEI T'CCGG_A Cuts at: 5397 5397 Size: 5734 BspHI T'CATG_A Cuts at: 3623 5353 3623 Size: 1730 4004 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2172 2665 2910 3089 844 Size: 1328 493 245 179 3489 Fragments arranged by size: 3489 1328 493 245 179 BsrBI CCG'CTC Cuts at: 1394 1823 3621 1394 Size: 429 1798 3507 Fragments arranged by size: 3507 1798 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4229 4403 65 Size: 1183 2981 174 1396 Fragments arranged by size: 2981 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4378 4565 1700 Size: 110 2568 187 2869 Fragments arranged by size: 2869 2568 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5734 BssHII G'CGCG_C Cuts at: 1925 2525 1925 Size: 600 5134 BssSI C'ACGA_G Cuts at: 3794 4806 3794 Size: 1012 4722 BstAPI GCAn_nnn'nTGC Cuts at: 794 2767 794 Size: 1973 3761 BstBI TT'CG_AA Cuts at: 2430 4538 2430 Size: 2108 3626 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5734 BstXI CCAn_nnnn'nTGG Cuts at: 2122 2122 Size: 5734 BstYI r'GATC_y Cuts at: 1972 2131 2456 3064 3431 3816 3833 5285 5371 Size: 159 325 608 367 385 17 1452 86 Cuts at: 5371 5383 5729 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 608 385 367 346 325 159 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2217 2912 513 Size: 552 1152 695 3335 Fragments arranged by size: 3335 1152 695 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4995 Fragments arranged by size: 4995 683 56 BtsI GCAGTG_nn' Cuts at: 3250 4044 4064 3250 Size: 794 20 4920 Fragments arranged by size: 4920 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5734 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5699 DraI TTT'AAA Cuts at: 3038 3038 Size: 5734 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4741 809 Size: 507 3425 1802 Fragments arranged by size: 3425 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2107 2513 2649 Size: 54 1425 50 275 18 277 406 136 Cuts at: 2649 3181 4070 5699 8 Size: 532 889 1629 43 Fragments arranged by size: 1629 1425 889 532 406 277 275 136 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3181 1537 Size: 275 1369 4090 Fragments arranged by size: 4090 1369 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2826 2991 3664 1353 Size: 1473 165 673 3423 Fragments arranged by size: 3423 1473 673 165 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2277 2728 4321 4695 4841 387 Size: 1064 138 688 451 1593 374 146 1280 Fragments arranged by size: 1593 1280 1064 688 451 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2098 2348 2375 2464 2612 3797 4394 Size: 393 466 250 27 89 148 1185 597 Cuts at: 4394 5181 5449 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1185 787 597 466 393 268 250 148 89 27 EcoNI CCTnn'n_nnAGG Cuts at: 2126 2126 Size: 5734 EcoO109I rG'GnC_Cy Cuts at: 2211 2212 2238 2261 2360 2502 2818 2819 2845 Size: 1 26 23 99 142 316 1 26 Cuts at: 2845 2908 2211 Size: 63 5037 Fragments arranged by size: 5037 316 142 99 63 26 26 23 1 1 EcoRI G'AATT_C Cuts at: 3043 3421 3043 Size: 378 5356 FspI TGC'GCA Cuts at: 4240 4240 Size: 5734 HaeII r_GCGC'y Cuts at: 1678 2591 4881 1678 Size: 913 2290 2531 Fragments arranged by size: 2531 2290 913 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2237 2269 4375 4407 4449 Size: 32 135 32 665 32 2106 32 42 Cuts at: 4449 4481 1373 Size: 32 2626 Fragments arranged by size: 2626 2106 665 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3078 3324 669 Size: 988 281 1140 246 3079 Fragments arranged by size: 3079 1140 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5734 HpaI GTT'AAC Cuts at: 3324 3324 Size: 5734 KpnI G_GTAC'C Cuts at: 2044 3059 2044 Size: 1015 4719 MfeI C'AATT_G Cuts at: 3333 3333 Size: 5734 MluI A'CGCG_T Cuts at: 2880 5610 2880 Size: 2730 3004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2595 2913 4848 5032 1305 Size: 1290 318 1935 184 2007 Fragments arranged by size: 2007 1935 1290 318 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3912 Fragments arranged by size: 3912 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3692 4051 4210 518 Size: 648 2526 359 159 2042 Fragments arranged by size: 2526 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2518 2581 2914 2929 3150 3827 4975 5220 Size: 912 63 333 15 221 677 1148 245 Cuts at: 5220 1606 Size: 2120 Fragments arranged by size: 2120 1148 912 677 333 245 221 63 15 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5182 NdeI CA'TA_TG Cuts at: 387 387 Size: 5734 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5734 NotI GC'GGCC_GC Cuts at: 3181 3181 Size: 5734 NruI TCG'CGA Cuts at: 2757 2757 Size: 5734 NspI r_CATG'y Cuts at: 1132 1229 2194 2551 3092 4637 1132 Size: 97 965 357 541 1545 2229 Fragments arranged by size: 2229 1545 965 541 357 97 PasI CC'CwG_GG Cuts at: 1348 2822 1348 Size: 1474 4260 PciI A'CATG_T Cuts at: 4633 4633 Size: 5734 PflMI CCAn_nnn'nTGG Cuts at: 1108 2869 1108 Size: 1761 3973 PmeI GTTT'AAAC Cuts at: 3038 3038 Size: 5734 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2585 2617 3803 3835 5316 5348 2585 Size: 32 1186 32 1481 32 2971 Fragments arranged by size: 2971 1481 1186 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2261 2502 2908 2261 Size: 241 406 5087 Fragments arranged by size: 5087 406 241 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5734 PsiI TTA'TAA Cuts at: 3304 3304 Size: 5734 PspOMI G'GGCC_C Cuts at: 2211 2360 2818 2845 2211 Size: 149 458 27 5100 Fragments arranged by size: 5100 458 149 27 PspXI vC'TCGA_Gb Cuts at: 2950 2950 Size: 5734 PstI C_TGCA'G Cuts at: 830 3086 830 Size: 2256 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5734 SacII CC_GC'GG Cuts at: 2915 2915 Size: 5734 SalI G'TCGA_C Cuts at: 1936 3076 1936 Size: 1140 4594 SanDI GG'GwC_CC Cuts at: 2261 2908 2261 Size: 647 5087 SbfI CC_TGCA'GG Cuts at: 3086 3086 Size: 5734 ScaI AGT'ACT Cuts at: 3982 5694 3982 Size: 1712 4022 SfcI C'TryA_G Cuts at: 826 1045 3082 4217 4898 5089 826 Size: 219 2037 1135 681 191 1471 Fragments arranged by size: 2037 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2219 2817 2219 Size: 598 5136 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5734 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5734 SmaI CCC'GGG Cuts at: 1872 2259 2500 2850 2962 3061 1872 Size: 387 241 350 112 99 4545 Fragments arranged by size: 4545 387 350 241 112 99 SmlI C'TyrA_G Cuts at: 820 1017 1943 2950 3838 4739 5001 820 Size: 197 926 1007 888 901 262 1553 Fragments arranged by size: 1553 1007 926 901 888 262 197 SnaBI TAC'GTA Cuts at: 493 3525 493 Size: 3032 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5734 SphI G_CATG'C Cuts at: 1132 2551 3092 1132 Size: 1419 541 3774 Fragments arranged by size: 3774 1419 541 SrfI GCCC'GGGC Cuts at: 2850 2850 Size: 5734 SspI AAT'ATT Cuts at: 5 52 3658 5 Size: 47 3606 2081 Fragments arranged by size: 3606 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5734 StyI C'CwwG_G Cuts at: 513 1065 2721 3173 513 Size: 552 1656 452 3074 Fragments arranged by size: 3074 1656 552 452 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3761 3778 3931 4116 4561 325 Size: 1255 2181 17 153 185 445 1498 Fragments arranged by size: 2181 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2616 3980 5692 Size: 274 80 33 51 1443 639 1364 1712 Cuts at: 5692 96 Size: 138 Fragments arranged by size: 1712 1443 1364 639 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2870 2902 5694 5726 2870 Size: 32 2792 32 2878 Fragments arranged by size: 2878 2792 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5734 XbaI T'CTAG_A Cuts at: 3070 3070 Size: 5734 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2122 2819 2866 2122 Size: 697 47 4990 Fragments arranged by size: 4990 697 47 XhoI C'TCGA_G Cuts at: 1943 2950 1943 Size: 1007 4727 XmaI C'CCGG_G Cuts at: 1870 2257 2498 2848 2960 3059 1870 Size: 387 241 350 112 99 4545 Fragments arranged by size: 4545 387 350 241 112 99 XmnI GAAnn'nnTTC Cuts at: 2600 3863 2600 Size: 1263 4471 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3542 276 Size: 53 83 186 936 2008 2468 Fragments arranged by size: 2468 2008 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PsrI PvuII RsrII SacI SapI SexAI SfoI SwaI