################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcnI BfrI BglI BlnI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BsrGI BssHII BssSI BstEII BstPI Bsu36I ClaI CpoI DraIII DrdI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI MaeII MluI MluNI MroI MscI NarI NciI NheI NotI NruI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 636 GTATAC GT^[AC][GT]AC AccII 63 CGCG CG^CG AhdI 2940 GACTCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 546 GGATC GGATC(4/5) AseI 985 ATTAAT AT^TAAT AsnI 985 ATTAAT AT^TAAT AspEI 2940 GACTCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 956 CTCGAG C^[CT]CG[GA]G BanI 34 GGCACC G^G[CT][GA]CC BbsI 1108 GAAGAC GAAGAC(2/6) BcgI 417 CGATAACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3046 GGATAC GTATCC(6/5) BlpI 2758 GCTAAGC GC^T[ACGT]AGC Bpu1102I 2758 GCTAAGC GC^T[ACGT]AGC BpuAI 1108 GAAGAC GAAGAC(2/6) BsaBI 2869 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsoBI 956 CTCGAG C^[CT]CG[GA]G BspHI 2493 TCATGA T^CATGA BspMI 163 GCAGGT ACCTGC(4/8) BsrBI 496 GAGCGG CCGCTC(-3/-3) BsrFI 2586 GCCGGC [GA]^CCGG[CT] Bst1107I 636 GTATAC GTA^TAC BstBI 2333 TTCGAA TT^CGAA BstUI 63 CGCG CG^CG BstZ17I 636 GTATAC GTA^TAC CelII 2758 GCTAAGC GC^T[ACGT]AGC Cfr10I 2586 GCCGGC [GA]^CCGG[CT] DraII 23 AGGCCCT [GA]G^G[ACGT]CC[CT] Eam1105I 2940 GACTCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 51 CCTGCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 23 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT22I 336 ATGCAT ATGCA^T FauI 30 GCGGG CCCGC(4/6) HaeII 68 AGCGCC [GA]GCGC^[CT] HapII 2587 CCGG C^CGG HpaII 2587 CCGG C^CGG KspI 62 CCGCGG CCGC^GG MamI 2869 GATGAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 2587 CCGG C^CGG MvnI 63 CGCG CG^CG NaeI 2586 GCCGGC GCC^GGC NgoMIV 2586 GCCGGC G^CCGGC NsiI 336 ATGCAT ATGCA^T NspV 2333 TTCGAA TT^CGAA PaeR7I 956 CTCGAG C^TCGAG PshBI 985 ATTAAT AT^TAAT PstI 2239 CTGCAG CTGCA^G PvuII 139 CAGCTG CAG^CTG RcaI 2493 TCATGA T^CATGA SacI 310 GAGCTC GAGCT^C SacII 62 CCGCGG CCGC^GG SfuI 2333 TTCGAA TT^CGAA XhoI 956 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 1364 ACATGT A^C[GA][CT]GT 1987 ACATGT AvaII 3 GGACC G^G[AT]CC 42 GGACC BanII 310 GAGCTC G[GA]GC[CT]^C 1292 GAGCCC BfaI 436 CTAG C^TAG 1405 CTAG BpmI 118 CTCCAG CTGGAG(16/14) 3062 CTCCAG BsiYI 51 CCTGCGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 3097 CCAGCACCAGG BslI 51 CCTGCGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 3097 CCAGCACCAGG BsmI 338 GCATTC GAATGC(1/-1) 2270 GCATTC BspLU11I 1364 ACATGT A^CATGT 1987 ACATGT BstAPI 525 GCATTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1813 GCAGTTACTGC BstXI 367 CCAAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2005 CCACAGATGTGG CfoI 69 GCGC GCG^C 1975 GCGC HhaI 69 GCGC GCG^C 1975 GCGC HinP1I 69 GCGC G^CGC 1975 GCGC HincII 1991 GTCAAC GT[CT]^[GA]AC 2473 GTTGAC HindII 1991 GTCAAC GT[CT]^[GA]AC 2473 GTTGAC HphI 2770 GGTGA GGTGA(8/7) 1010 TCACC MaeI 436 CTAG C^TAG 1405 CTAG MfeI 1841 CAATTG C^AATTG 2357 CAATTG MunI 1841 CAATTG C^AATTG 2357 CAATTG NcoI 38 CCATGG C^CATGG 766 CCATGG NdeI 1300 CATATG CA^TATG 2624 CATATG SapI 3005 GAAGAGC GCTCTTC(1/4) 1633 GCTCTTC SfcI 1200 CTGTAG C^T[GA][CT]AG 2239 CTGCAG SmlI 956 CTCGAG C^T[CT][GA]AG 1894 CTTGAG SspI 280 AATATT AAT^ATT 2741 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApoI Asp700I AspHI BbvI BclI BglII BmyI BsaJI BseRI BsiHKAI BsmAI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DsaI EarI EcoRI EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII XmnI