(Circular) (Six-base) MAPSORT of: pFN21AE4259.seq Check: 5474 from: 1 to: 7819 pFN21AE4259 7819 bp With 182 enzymes: SgfI * March 28, 2012 13:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2159 2159 Size: 7819 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5629 278 Size: 53 83 186 936 4093 2468 Fragments arranged by size: 4093 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2642 5162 1937 Size: 705 2520 4594 Fragments arranged by size: 4594 2520 705 Acc65I G'GTAC_C Cuts at: 5140 5140 Size: 7819 AclI AA'CG_TT Cuts at: 1563 5946 6319 1563 Size: 4383 373 3063 Fragments arranged by size: 4383 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2399 2892 2903 3050 3356 3382 4701 4836 Size: 767 493 11 147 306 26 1319 135 Cuts at: 4836 5882 7266 7534 1632 Size: 1046 1384 268 1917 Fragments arranged by size: 1917 1384 1319 1046 767 493 306 268 147 135 26 11 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7622 AflIII A'CryG_T Cuts at: 1203 3369 3992 6718 7695 1203 Size: 2166 623 2726 977 1327 Fragments arranged by size: 2726 2166 1327 977 623 AgeI A'CCGG_T Cuts at: 6650 6650 Size: 7819 AhdI GACnn_n'nnGTC Cuts at: 4950 6548 4950 Size: 1598 6221 AleI CACnn'nnGTG Cuts at: 1166 4015 1166 Size: 2849 4970 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3448 4651 1184 Size: 2264 1203 4352 Fragments arranged by size: 4352 2264 1203 AlwNI CAG_nnn'CTG Cuts at: 2677 3092 3824 4942 5562 7134 2677 Size: 415 732 1118 620 1572 3362 Fragments arranged by size: 3362 1572 1118 732 620 415 ApaLI G'TGCA_C Cuts at: 5876 7032 5876 Size: 1156 6663 ApoI r'AATT_y Cuts at: 1493 2104 2445 2694 2864 3834 3953 5057 5128 Size: 611 341 249 170 970 119 1104 71 Cuts at: 5128 5354 5506 7615 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1104 970 611 341 249 226 170 152 119 71 AseI AT'TA_AT Cuts at: 160 1032 2991 6373 160 Size: 872 1959 3382 1606 Fragments arranged by size: 3382 1959 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7819 AvaI C'yCGr_G Cuts at: 1870 1943 2961 5144 1870 Size: 73 1018 2183 4545 Fragments arranged by size: 4545 2183 1018 73 BamHI G'GATC_C Cuts at: 5149 5516 5149 Size: 367 7452 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 5140 6595 618 Size: 325 217 627 252 3101 1455 1842 Fragments arranged by size: 3101 1842 1455 627 325 252 217 BanII G_rGCy'C Cuts at: 1391 2319 3301 5138 1391 Size: 928 982 1837 4072 Fragments arranged by size: 4072 1837 982 928 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3120 928 Size: 971 1221 5627 Fragments arranged by size: 5627 1221 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2411 2445 6010 6044 1903 Size: 34 474 34 3565 34 3678 Fragments arranged by size: 3678 3565 474 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5045 5711 6921 5045 Size: 666 1210 5943 Fragments arranged by size: 5943 1210 666 BclI T'GATC_A Cuts at: 1548 2584 3475 3576 5002 1548 Size: 1036 891 101 1426 4365 Fragments arranged by size: 4365 1426 1036 891 101 BfrBI ATG'CAT Cuts at: 2343 2343 Size: 7819 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6430 136 Size: 107 122 71 631 605 4758 1525 Fragments arranged by size: 4758 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2619 3345 3966 7814 2619 Size: 726 621 3848 2624 Fragments arranged by size: 3848 2624 726 621 BlpI GC'TnA_GC Cuts at: 4764 5236 4764 Size: 472 7347 Bme1580I G_kGCm'C Cuts at: 1790 2042 5880 7036 1790 Size: 252 3838 1156 2573 Fragments arranged by size: 3838 2573 1156 252 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7819 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4715 5550 6508 Size: 416 53 83 399 24 3298 835 958 Cuts at: 6508 442 Size: 1753 Fragments arranged by size: 3298 1753 958 835 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7819 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3988 3988 Size: 7819 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 5052 6479 1239 Size: 869 2944 1427 2579 Fragments arranged by size: 2944 2579 1427 869 Bpu10I CC'TnA_GC Cuts at: 2313 2313 Size: 7819 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3920 5944 6809 7107 3920 Size: 2024 865 298 4632 Fragments arranged by size: 4632 2024 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6482 882 Size: 617 4983 2219 Fragments arranged by size: 4983 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5610 493 Size: 711 4406 2702 Fragments arranged by size: 4406 2702 711 BsaBI GATnn'nnATC Cuts at: 4878 7813 4878 Size: 2935 4884 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5626 6008 6643 275 Size: 53 83 186 936 4093 382 635 1451 Fragments arranged by size: 4093 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6252 6650 6924 7071 7482 6252 Size: 398 274 147 411 6589 Fragments arranged by size: 6589 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7594 Fragments arranged by size: 7594 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2513 2949 3977 1186 Size: 1327 436 1028 5028 Fragments arranged by size: 5028 1327 1028 436 BseYI C'CCAG_C Cuts at: 2050 3435 5101 7022 7518 2050 Size: 1385 1666 1921 496 2351 Fragments arranged by size: 2351 1921 1666 1385 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7246 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5269 6030 7058 1540 Size: 275 186 3268 761 1028 2301 Fragments arranged by size: 3268 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2319 4534 5068 5880 5965 7036 2319 Size: 2215 534 812 85 1071 3102 Fragments arranged by size: 3102 2215 1071 812 534 85 BsmI GAATG_Cn' Cuts at: 2343 4275 5330 5423 2343 Size: 1932 1055 93 4739 Fragments arranged by size: 4739 1932 1055 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2319 3301 4534 5068 5138 5880 Size: 399 252 277 982 1233 534 70 742 Cuts at: 5880 5965 7036 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1233 1071 982 742 534 399 277 252 85 70 BspEI T'CCGG_A Cuts at: 7482 7482 Size: 7819 BspHI T'CATG_A Cuts at: 4498 5708 7438 4498 Size: 1210 1730 4879 Fragments arranged by size: 4879 1730 1210 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2159 5174 844 Size: 1315 3015 3489 Fragments arranged by size: 3489 3015 1315 BsrBI CCG'CTC Cuts at: 1394 1823 2503 5706 1394 Size: 429 680 3203 3507 Fragments arranged by size: 3507 3203 680 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2529 2848 3558 3765 6314 6488 65 Size: 1183 1281 319 710 207 2549 174 1396 Fragments arranged by size: 2549 1396 1281 1183 710 319 207 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4591 6463 6650 1700 Size: 110 2781 1872 187 2869 Fragments arranged by size: 2869 2781 1872 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7819 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7819 BssSI C'ACGA_G Cuts at: 5879 6891 5879 Size: 1012 6807 BstAPI GCAn_nnn'nTGC Cuts at: 794 2536 3824 794 Size: 1742 1288 4789 Fragments arranged by size: 4789 1742 1288 BstBI TT'CG_AA Cuts at: 4339 6623 4339 Size: 2284 5535 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7819 BstXI CCAn_nnnn'nTGG Cuts at: 2379 4017 2379 Size: 1638 6181 BstYI r'GATC_y Cuts at: 1972 2619 3345 3966 5149 5516 5901 5918 7370 Size: 647 726 621 1183 367 385 17 1452 Cuts at: 7370 7456 7468 7814 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1183 726 647 621 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2643 2643 Size: 7819 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 2067 2771 513 Size: 552 939 39 24 704 5561 Fragments arranged by size: 5561 939 704 552 39 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7080 Fragments arranged by size: 7080 683 56 BtsI GCAGTG_nn' Cuts at: 2117 3174 3409 4253 5335 6129 6149 2117 Size: 1057 235 844 1082 794 20 3787 Fragments arranged by size: 3787 1082 1057 844 794 235 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7819 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4240 4275 4831 4866 529 Size: 35 3676 35 556 35 3482 Fragments arranged by size: 3676 3482 556 35 35 35 DraI TTT'AAA Cuts at: 2700 4566 4891 5123 2700 Size: 1866 325 232 5396 Fragments arranged by size: 5396 1866 325 232 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6826 809 Size: 507 5510 1802 Fragments arranged by size: 5510 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5266 6155 7784 Size: 54 1425 50 275 18 3436 889 1629 Cuts at: 7784 8 Size: 43 Fragments arranged by size: 3436 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5266 1537 Size: 275 3454 4090 Fragments arranged by size: 4090 3454 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3401 3645 4812 5005 5749 1353 Size: 2048 244 1167 193 744 3423 Fragments arranged by size: 3423 2048 1167 744 244 193 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6406 6780 6926 387 Size: 1064 138 4817 374 146 1280 Fragments arranged by size: 4817 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2317 2317 Size: 7819 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2399 2892 2903 3050 3356 3382 Size: 393 476 291 493 11 147 306 26 Cuts at: 3382 4701 4836 5052 5882 6479 7266 7534 1239 Size: 1319 135 216 830 597 787 268 1524 Fragments arranged by size: 1524 1319 830 787 597 493 476 393 306 291 268 216 147 135 26 11 EcoNI CCTnn'n_nnAGG Cuts at: 2060 2060 Size: 7819 EcoO109I rG'GnC_Cy Cuts at: 2029 2029 Size: 7819 EcoRI G'AATT_C Cuts at: 2445 3834 3953 5128 5506 2445 Size: 1389 119 1175 378 4758 Fragments arranged by size: 4758 1389 1175 378 119 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2812 2812 Size: 7819 FspI TGC'GCA Cuts at: 6325 6325 Size: 7819 HaeII r_GCGC'y Cuts at: 1678 2077 6966 1678 Size: 399 4889 2531 Fragments arranged by size: 4889 2531 399 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3568 3600 4936 4968 6460 Size: 32 135 32 1996 32 1336 32 1492 Cuts at: 6460 6492 6534 6566 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1996 1492 1336 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3998 4480 5163 5409 669 Size: 988 281 2060 482 683 246 3079 Fragments arranged by size: 3079 2060 988 683 482 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7819 HpaI GTT'AAC Cuts at: 5409 5409 Size: 7819 KpnI G_GTAC'C Cuts at: 5144 5144 Size: 7819 MfeI C'AATT_G Cuts at: 3846 4362 5418 3846 Size: 516 1056 6247 Fragments arranged by size: 6247 1056 516 MluI A'CGCG_T Cuts at: 7695 7695 Size: 7819 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2118 6933 7117 1305 Size: 813 4815 184 2007 Fragments arranged by size: 4815 2007 813 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5997 Fragments arranged by size: 5997 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3076 4015 4101 4653 5777 6136 6295 Size: 648 1910 939 86 552 1124 359 159 Cuts at: 6295 518 Size: 2042 Fragments arranged by size: 2042 1910 1124 939 648 552 359 159 86 MspA1I CmG'CkG Cuts at: 1606 2069 2146 2379 5235 5912 7060 7305 1606 Size: 463 77 233 2856 677 1148 245 2120 Fragments arranged by size: 2856 2120 1148 677 463 245 233 77 NaeI GCC'GGC Cuts at: 4593 4593 Size: 7819 NcoI C'CATG_G Cuts at: 513 1065 2004 2043 2771 513 Size: 552 939 39 728 5561 Fragments arranged by size: 5561 939 728 552 39 NdeI CA'TA_TG Cuts at: 387 3306 4630 387 Size: 2919 1324 3576 Fragments arranged by size: 3576 2919 1324 NgoMIV G'CCGG_C Cuts at: 4591 4591 Size: 7819 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7819 NotI GC'GGCC_GC Cuts at: 5266 5266 Size: 7819 NsiI A_TGCA'T Cuts at: 2345 2345 Size: 7819 NspI r_CATG'y Cuts at: 1132 1229 2343 3373 3947 3996 5177 6722 1132 Size: 97 1114 1030 574 49 1181 1545 2229 Fragments arranged by size: 2229 1545 1181 1114 1030 574 97 49 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7819 PciI A'CATG_T Cuts at: 3369 3992 6718 3369 Size: 623 2726 4470 Fragments arranged by size: 4470 2726 623 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7819 PmeI GTTT'AAAC Cuts at: 5123 5123 Size: 7819 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5888 5920 7401 7433 5888 Size: 32 1481 32 6274 Fragments arranged by size: 6274 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7819 PsiI TTA'TAA Cuts at: 3653 4380 5389 3653 Size: 727 1009 6083 Fragments arranged by size: 6083 1009 727 PspXI vC'TCGA_Gb Cuts at: 2961 2961 Size: 7819 PstI C_TGCA'G Cuts at: 830 4248 5171 830 Size: 3418 923 3478 Fragments arranged by size: 3478 3418 923 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7819 PvuII CAG'CTG Cuts at: 2146 2146 Size: 7819 SacI G_AGCT'C Cuts at: 2319 2319 Size: 7819 SacII CC_GC'GG Cuts at: 2070 2070 Size: 7819 SalI G'TCGA_C Cuts at: 1936 5161 1936 Size: 3225 4594 SapI GCTCTTCn'nnn_ Cuts at: 3645 5005 3645 Size: 1360 6459 SbfI CC_TGCA'GG Cuts at: 5171 5171 Size: 7819 ScaI AGT'ACT Cuts at: 6067 7779 6067 Size: 1712 6107 SfcI C'TryA_G Cuts at: 826 1045 3205 4244 5167 6302 6983 7174 826 Size: 219 2160 1039 923 1135 681 191 1471 Fragments arranged by size: 2160 1471 1135 1039 923 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7819 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7819 SmaI CCC'GGG Cuts at: 1872 5146 1872 Size: 3274 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2961 3899 5923 6824 7086 820 Size: 197 926 1018 938 2024 901 262 1553 Fragments arranged by size: 2024 1553 1018 938 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5610 493 Size: 5117 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7819 SphI G_CATG'C Cuts at: 1132 5177 1132 Size: 4045 3774 SspI AAT'ATT Cuts at: 5 52 2287 4748 5743 5 Size: 47 2235 2461 995 2081 Fragments arranged by size: 2461 2235 2081 995 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7819 StyI C'CwwG_G Cuts at: 513 1065 2004 2043 2771 2850 5258 513 Size: 552 939 39 728 79 2408 3074 Fragments arranged by size: 3074 2408 939 728 552 79 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5846 5863 6016 6201 6646 325 Size: 1255 4266 17 153 185 445 1498 Fragments arranged by size: 4266 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2934 6065 7777 Size: 274 80 33 51 1443 957 3131 1712 Cuts at: 7777 96 Size: 138 Fragments arranged by size: 3131 1712 1443 957 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7779 7811 7779 Size: 32 7787 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7819 XbaI T'CTAG_A Cuts at: 5155 5155 Size: 7819 XhoI C'TCGA_G Cuts at: 1943 2961 1943 Size: 1018 6801 XmaI C'CCGG_G Cuts at: 1870 5144 1870 Size: 3274 4545 XmnI GAAnn'nnTTC Cuts at: 2736 2877 3330 4047 4413 5948 2736 Size: 141 453 717 366 1535 4607 Fragments arranged by size: 4607 1535 717 453 366 141 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5627 276 Size: 53 83 186 936 4093 2468 Fragments arranged by size: 4093 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI XcmI