(Linear) (Six-base) MAPSORT of: KIEE4259.seq Check: 868 from: 1 to: 3114 KIEE4259.orf With 182 enzymes: SgfI * March 22, 2012 17:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 154 3114 Size: 154 2960 AccI GT'mk_AC Cuts at: 0 637 3114 Size: 637 2477 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 394 887 898 1045 1351 1377 2696 2831 Size: 394 493 11 147 306 26 1319 135 Cuts at: 2831 3114 Size: 283 Fragments arranged by size: 1319 493 394 306 283 147 135 26 11 AflIII A'CryG_T Cuts at: 0 1364 1987 3114 Size: 1364 623 1127 Fragments arranged by size: 1364 1127 623 AhdI GACnn_n'nnGTC Cuts at: 0 2945 3114 Size: 2945 169 AleI CACnn'nnGTG Cuts at: 0 2010 3114 Size: 2010 1104 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1443 2646 3114 Size: 1443 1203 468 Fragments arranged by size: 1443 1203 468 AlwNI CAG_nnn'CTG Cuts at: 0 672 1087 1819 2937 3114 Size: 672 415 732 1118 177 Fragments arranged by size: 1118 732 672 415 177 ApoI r'AATT_y Cuts at: 0 99 440 689 859 1829 1948 3052 3114 Size: 99 341 249 170 970 119 1104 62 Fragments arranged by size: 1104 970 341 249 170 119 99 62 AseI AT'TA_AT Cuts at: 0 986 3114 Size: 986 2128 AvaI C'yCGr_G Cuts at: 0 956 3114 Size: 956 2158 BanI G'GyrC_C Cuts at: 0 34 3114 Size: 34 3080 BanII G_rGCy'C Cuts at: 0 314 1296 3114 Size: 314 982 1818 Fragments arranged by size: 1818 982 314 BbsI GAAGACnn'nnnn_ Cuts at: 0 1115 3114 Size: 1115 1999 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 406 440 3114 Size: 406 34 2674 Fragments arranged by size: 2674 406 34 BciVI GTATCCnnnnn_n' Cuts at: 0 3040 3114 Size: 3040 74 BclI T'GATC_A Cuts at: 0 579 1470 1571 2997 3114 Size: 579 891 101 1426 117 Fragments arranged by size: 1426 891 579 117 101 BfrBI ATG'CAT Cuts at: 0 338 3114 Size: 338 2776 BglII A'GATC_T Cuts at: 0 614 1340 1961 3114 Size: 614 726 621 1153 Fragments arranged by size: 1153 726 621 614 BlpI GC'TnA_GC Cuts at: 0 2759 3114 Size: 2759 355 Bme1580I G_kGCm'C Cuts at: 0 37 3114 Size: 37 3077 BmrI ACTGGGnnnn_n' Cuts at: 0 2710 3114 Size: 2710 404 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1983 3114 Size: 1983 1131 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 3047 3114 Size: 103 2944 67 Fragments arranged by size: 2944 103 67 Bpu10I CC'TnA_GC Cuts at: 0 308 3114 Size: 308 2806 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1915 3114 Size: 1915 1199 BsaBI GATnn'nnATC Cuts at: 0 2873 3114 Size: 2873 241 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 508 944 1972 3114 Size: 508 436 1028 1142 Fragments arranged by size: 1142 1028 508 436 BseYI C'CCAG_C Cuts at: 0 45 1430 3096 3114 Size: 45 1385 1666 18 Fragments arranged by size: 1666 1385 45 18 BsiHKAI G_wGCw'C Cuts at: 0 314 2529 3063 3114 Size: 314 2215 534 51 Fragments arranged by size: 2215 534 314 51 BsmI GAATG_Cn' Cuts at: 0 338 2270 3114 Size: 338 1932 844 Fragments arranged by size: 1932 844 338 Bsp1286I G_dGCh'C Cuts at: 0 37 314 1296 2529 3063 3114 Size: 37 277 982 1233 534 51 Fragments arranged by size: 1233 982 534 277 51 37 BspHI T'CATG_A Cuts at: 0 2493 3114 Size: 2493 621 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 154 3114 Size: 154 2960 BsrBI CCG'CTC Cuts at: 0 498 3114 Size: 498 2616 BsrDI GCAATG_nn' Cuts at: 0 524 843 1553 1760 3114 Size: 524 319 710 207 1354 Fragments arranged by size: 1354 710 524 319 207 BsrFI r'CCGG_y Cuts at: 0 2586 3114 Size: 2586 528 BstAPI GCAn_nnn'nTGC Cuts at: 0 531 1819 3114 Size: 531 1288 1295 Fragments arranged by size: 1295 1288 531 BstBI TT'CG_AA Cuts at: 0 2334 3114 Size: 2334 780 BstXI CCAn_nnnn'nTGG Cuts at: 0 374 2012 3114 Size: 374 1638 1102 Fragments arranged by size: 1638 1102 374 BstYI r'GATC_y Cuts at: 0 614 1340 1961 3114 Size: 614 726 621 1153 Fragments arranged by size: 1153 726 621 614 BstZ17I GTA'TAC Cuts at: 0 638 3114 Size: 638 2476 BtgI C'CryG_G Cuts at: 0 38 62 766 3114 Size: 38 24 704 2348 Fragments arranged by size: 2348 704 38 24 BtsI GCAGTG_nn' Cuts at: 0 112 1169 1404 2248 3114 Size: 112 1057 235 844 866 Fragments arranged by size: 1057 866 844 235 112 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2235 2270 2826 2861 3114 Size: 2235 35 556 35 253 Fragments arranged by size: 2235 556 253 35 35 DraI TTT'AAA Cuts at: 0 695 2561 2886 3114 Size: 695 1866 325 228 Fragments arranged by size: 1866 695 325 228 EarI CTCTTCn'nnn_ Cuts at: 0 1396 1640 2807 3000 3114 Size: 1396 244 1167 193 114 Fragments arranged by size: 1396 1167 244 193 114 EcoICRI GAG'CTC Cuts at: 0 312 3114 Size: 312 2802 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 394 887 898 1045 1351 1377 2696 Size: 103 291 493 11 147 306 26 1319 Cuts at: 2696 2831 3047 3114 Size: 135 216 67 Fragments arranged by size: 1319 493 306 291 216 147 135 103 67 26 11 EcoNI CCTnn'n_nnAGG Cuts at: 0 55 3114 Size: 55 3059 EcoO109I rG'GnC_Cy Cuts at: 0 24 3114 Size: 24 3090 EcoRI G'AATT_C Cuts at: 0 440 1829 1948 3114 Size: 440 1389 119 1166 Fragments arranged by size: 1389 1166 440 119 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 807 3114 Size: 807 2307 HaeII r_GCGC'y Cuts at: 0 72 3114 Size: 72 3042 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1563 1595 2931 2963 3114 Size: 1563 32 1336 32 151 Fragments arranged by size: 1563 1336 151 32 32 HincII GTy'rAC Cuts at: 0 1993 2475 3114 Size: 1993 482 639 Fragments arranged by size: 1993 639 482 MfeI C'AATT_G Cuts at: 0 1841 2357 3114 Size: 1841 516 757 Fragments arranged by size: 1841 757 516 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 113 3114 Size: 113 3001 MslI CAynn'nnrTG Cuts at: 0 1071 2010 2096 2648 3114 Size: 1071 939 86 552 466 Fragments arranged by size: 1071 939 552 466 86 MspA1I CmG'CkG Cuts at: 0 64 141 374 3114 Size: 64 77 233 2740 Fragments arranged by size: 2740 233 77 64 NaeI GCC'GGC Cuts at: 0 2588 3114 Size: 2588 526 NcoI C'CATG_G Cuts at: 0 38 766 3114 Size: 38 728 2348 Fragments arranged by size: 2348 728 38 NdeI CA'TA_TG Cuts at: 0 1301 2625 3114 Size: 1301 1324 489 Fragments arranged by size: 1324 1301 489 NgoMIV G'CCGG_C Cuts at: 0 2586 3114 Size: 2586 528 NsiI A_TGCA'T Cuts at: 0 340 3114 Size: 340 2774 NspI r_CATG'y Cuts at: 0 338 1368 1942 1991 3114 Size: 338 1030 574 49 1123 Fragments arranged by size: 1123 1030 574 338 49 PciI A'CATG_T Cuts at: 0 1364 1987 3114 Size: 1364 623 1127 Fragments arranged by size: 1364 1127 623 PsiI TTA'TAA Cuts at: 0 1648 2375 3114 Size: 1648 727 739 Fragments arranged by size: 1648 739 727 PspXI vC'TCGA_Gb Cuts at: 0 956 3114 Size: 956 2158 PstI C_TGCA'G Cuts at: 0 2243 3114 Size: 2243 871 PvuII CAG'CTG Cuts at: 0 141 3114 Size: 141 2973 SacI G_AGCT'C Cuts at: 0 314 3114 Size: 314 2800 SacII CC_GC'GG Cuts at: 0 65 3114 Size: 65 3049 SapI GCTCTTCn'nnn_ Cuts at: 0 1640 3000 3114 Size: 1640 1360 114 Fragments arranged by size: 1640 1360 114 SfcI C'TryA_G Cuts at: 0 1200 2239 3114 Size: 1200 1039 875 Fragments arranged by size: 1200 1039 875 SmlI C'TyrA_G Cuts at: 0 956 1894 3114 Size: 956 938 1220 Fragments arranged by size: 1220 956 938 SspI AAT'ATT Cuts at: 0 282 2743 3114 Size: 282 2461 371 Fragments arranged by size: 2461 371 282 StyI C'CwwG_G Cuts at: 0 38 766 845 3114 Size: 38 728 79 2269 Fragments arranged by size: 2269 728 79 38 TatI w'GTAC_w Cuts at: 0 929 3114 Size: 929 2185 XhoI C'TCGA_G Cuts at: 0 956 3114 Size: 956 2158 XmnI GAAnn'nnTTC Cuts at: 0 731 872 1325 2042 2408 3114 Size: 731 141 453 717 366 706 Fragments arranged by size: 731 717 706 453 366 141 Enzymes that do cut: AarI AccI AcuI AflIII AhdI AleI AlfI AlwNI ApoI AseI AvaI BanI BanII BbsI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaBI BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstXI BstYI BstZ17I BtgI BtsI CspCI DraI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII MfeI MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PciI PsiI PspXI PstI PvuII SacI SacII SapI SfcI SmlI SspI StyI TatI XhoI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BglI BmgBI BmtI BsaI BsaAI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BsrGI BssHII BssSI BstEII Bsu36I BtgZI ClaI DraIII DrdI EaeI EagI EciI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MluI MscI NarI NheI NotI NruI PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI Tth111I XbaI XcmI XmaI ZraI