################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HpaI HphI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 590 GTCTAC GT^[AC][GT]AC AlwNI 548 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 505 GAGCAC G[AT]GC[AT]^C AvaII 235 GGTCC G^G[AT]CC BanI 197 GGCGCC G^G[CT][GA]CC BbeI 197 GGCGCC GGCGC^C BcnI 476 CCCGG CC^[GC]GG BfaI 511 CTAG C^TAG BsaI 40 GAGACC GGTCTC(1/5) BsgI 97 GTGCAG GTGCAG(16/14) BsiEI 30 CGGCCG CG[GA][CT]^CG BsiHKAI 505 GAGCAC G[AT]GC[AT]^C BspMI 125 ACCTGC ACCTGC(4/8) BsrFI 638 ACCGGC [GA]^CCGG[CT] BstAPI 90 GCAGGCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 336 CCAGTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 90 GCAGGC GC[ACGT]^[ACGT]GC Cfr10I 638 ACCGGC [GA]^CCGG[CT] DraIII 508 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 30 CGGCCG [CT]^GGCC[GA] EagI 30 CGGCCG C^GGCCG EclXI 30 CGGCCG C^GGCCG Eco52I 30 CGGCCG C^GGCCG EcoNI 18 CCTCACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 51 GCGGG CCCGC(4/6) HgaI 61 GCGTC GACGC(5/10) HincII 367 GTTGAC GT[CT]^[GA]AC HindII 367 GTTGAC GT[CT]^[GA]AC HindIII 463 AAGCTT A^AGCTT KasI 197 GGCGCC G^GCGCC MaeI 511 CTAG C^TAG MaeIII 63 GTCAC ^GT[ACGT]AC MslI 445 CACAAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 551 CAGCTG C[AC]G^C[GT]G NarI 197 GGCGCC GG^CGCC NciI 476 CCCGG CC^[GC]GG NotI 29 GCGGCCGC GC^GGCCGC PstI 190 CTGCAG CTGCA^G PvuII 551 CAGCTG CAG^CTG SfoI 197 GGCGCC GGC^GCC SspI 184 AATATT AAT^ATT Tsp45I 63 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 60 GGCGTC G[GA]^CG[CT]C 197 GGCGCC BbsI 654 GAAGAC GAAGAC(2/6) 703 GAAGAC BclI 515 TGATCA T^GATCA 644 TGATCA BpuAI 654 GAAGAC GAAGAC(2/6) 703 GAAGAC BsaHI 60 GGCGTC G[GA]^CG[CT]C 197 GGCGCC BseRI 16 CTCCTC GAGGAG(10/8) 307 GAGGAG BsrI 522 ACTGG ACTGG(1/-1) 336 CCAGT BstNI 135 CCTGG CC^[AT]GG 537 CCTGG CfoI 78 GCGC GCG^C 198 GCGC EcoRII 135 CCTGG ^CC[AT]GG 537 CCTGG FbaI 515 TGATCA T^GATCA 644 TGATCA HaeII 77 AGCGCC [GA]GCGC^[CT] 197 GGCGCC HhaI 78 GCGC GCG^C 198 GCGC Hin1I 60 GGCGTC G[GA]^CG[CT]C 197 GGCGCC HinP1I 78 GCGC G^CGC 198 GCGC MvaI 135 CCTGG CC^[AT]GG 537 CCTGG SfaNI 480 GCATC GCATC(5/9) 617 GCATC SfcI 190 CTGCAG C^T[GA][CT]AG 592 CTACAG SmlI 145 CTCAAG C^T[CT][GA]AG 424 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AluI BanII BbvI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I Cfr13I DdeI DpnII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboI MboII MnlI MspI MwoI NdeII NlaIII NlaIV NspI PleI Sau3AI Sau96I ScrFI StyI TseI TspRI