(Circular) (Six-base) MAPSORT of: pFN21AE6259.seq Check: 9409 from: 1 to: 5428 pFN21AE6259 5428 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3238 278 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2596 2771 1937 Size: 659 175 4594 Fragments arranged by size: 4594 659 175 Acc65I G'GTAC_C Cuts at: 2749 2749 Size: 5428 AclI AA'CG_TT Cuts at: 1563 3555 3928 1563 Size: 1992 373 3063 Fragments arranged by size: 3063 1992 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2636 2703 3491 4875 5143 1632 Size: 1004 67 788 1384 268 1917 Fragments arranged by size: 1917 1384 1004 788 268 67 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5231 AflIII A'CryG_T Cuts at: 1203 4327 5304 1203 Size: 3124 977 1327 Fragments arranged by size: 3124 1327 977 AgeI A'CCGG_T Cuts at: 4259 4259 Size: 5428 AhdI GACnn_n'nnGTC Cuts at: 4157 4157 Size: 5428 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5428 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5428 AlwNI CAG_nnn'CTG Cuts at: 2558 3171 4743 2558 Size: 613 1572 3243 Fragments arranged by size: 3243 1572 613 ApaLI G'TGCA_C Cuts at: 3485 4641 3485 Size: 1156 4272 ApoI r'AATT_y Cuts at: 1493 2737 2963 3115 5224 1493 Size: 1244 226 152 2109 1697 Fragments arranged by size: 2109 1697 1244 226 152 AseI AT'TA_AT Cuts at: 160 1032 3982 160 Size: 872 2950 1606 Fragments arranged by size: 2950 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 AvaI C'yCGr_G Cuts at: 1870 1943 2753 1870 Size: 73 810 4545 Fragments arranged by size: 4545 810 73 BamHI G'GATC_C Cuts at: 2758 3125 2758 Size: 367 5061 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2202 2749 4204 618 Size: 325 217 627 415 547 1455 1842 Fragments arranged by size: 1842 1455 627 547 415 325 217 BanII G_rGCy'C Cuts at: 1391 2022 2165 2534 2747 1391 Size: 631 143 369 213 4072 Fragments arranged by size: 4072 631 369 213 143 BbeI G_GCGC'C Cuts at: 2206 2206 Size: 5428 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2666 2715 928 Size: 971 767 49 3641 Fragments arranged by size: 3641 971 767 49 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3619 3653 1903 Size: 34 1682 34 3678 Fragments arranged by size: 3678 1682 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3320 4530 3320 Size: 1210 4218 BclI T'GATC_A Cuts at: 1548 2520 2649 1548 Size: 972 129 4327 Fragments arranged by size: 4327 972 129 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4039 136 Size: 107 122 71 631 605 2367 1525 Fragments arranged by size: 2367 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5423 5423 Size: 5428 BlpI GC'TnA_GC Cuts at: 2845 2845 Size: 5428 Bme1580I G_kGCm'C Cuts at: 1790 3489 4645 1790 Size: 1699 1156 2573 Fragments arranged by size: 2573 1699 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5428 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2537 3159 4117 Size: 416 53 83 399 24 1120 622 958 Cuts at: 4117 442 Size: 1753 Fragments arranged by size: 1753 1120 958 622 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5428 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2713 2713 Size: 5428 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4088 1239 Size: 2849 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2135 2414 3553 4418 4716 2135 Size: 279 1139 865 298 2847 Fragments arranged by size: 2847 1139 865 298 279 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2039 4091 882 Size: 617 540 2052 2219 Fragments arranged by size: 2219 2052 617 540 BsaAI yAC'GTr Cuts at: 493 1204 3219 493 Size: 711 2015 2702 Fragments arranged by size: 2702 2015 711 BsaBI GATnn'nnATC Cuts at: 5422 5422 Size: 5428 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2066 2203 3235 3617 Size: 53 83 186 936 533 137 1032 382 Cuts at: 3617 4252 275 Size: 635 1451 Fragments arranged by size: 1451 1032 936 635 533 382 186 137 83 53 BsaWI w'CCGG_w Cuts at: 3861 4259 4533 4680 5091 3861 Size: 398 274 147 411 4198 Fragments arranged by size: 4198 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2051 2081 2105 2135 2589 Size: 30 165 30 648 30 24 30 454 Cuts at: 2589 2619 1178 Size: 30 3987 Fragments arranged by size: 3987 648 454 165 30 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2012 2327 1186 Size: 826 315 4287 Fragments arranged by size: 4287 826 315 BseYI C'CCAG_C Cuts at: 2158 2551 4631 5127 2158 Size: 393 2080 496 2459 Fragments arranged by size: 2459 2080 496 393 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 1163 Size: 573 387 4468 Fragments arranged by size: 4468 573 387 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2038 2878 3639 4667 1540 Size: 275 186 37 840 761 1028 2301 Fragments arranged by size: 2301 1028 840 761 275 186 37 BsiHKAI G_wGCw'C Cuts at: 2514 3489 3574 4645 2514 Size: 975 85 1071 3297 Fragments arranged by size: 3297 1071 975 85 BsmI GAATG_Cn' Cuts at: 2939 3032 2939 Size: 93 5335 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2165 2514 2534 2747 3489 3574 Size: 399 232 143 349 20 213 742 85 Cuts at: 3574 4645 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 349 232 213 143 85 20 BspEI T'CCGG_A Cuts at: 5091 5091 Size: 5428 BspHI T'CATG_A Cuts at: 3317 5047 3317 Size: 1730 3698 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2139 2783 844 Size: 1295 644 3489 Fragments arranged by size: 3489 1295 644 BsrBI CCG'CTC Cuts at: 1394 1823 3315 1394 Size: 429 1492 3507 Fragments arranged by size: 3507 1492 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3923 4097 65 Size: 1183 2675 174 1396 Fragments arranged by size: 2675 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2643 4072 4259 1700 Size: 110 833 1429 187 2869 Fragments arranged by size: 2869 1429 833 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5428 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5428 BssSI C'ACGA_G Cuts at: 3488 4500 3488 Size: 1012 4416 BstAPI GCAn_nnn'nTGC Cuts at: 794 2101 794 Size: 1307 4121 BstBI TT'CG_AA Cuts at: 4232 4232 Size: 5428 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5428 BstXI CCAn_nnnn'nTGG Cuts at: 2348 2348 Size: 5428 BstYI r'GATC_y Cuts at: 1972 2758 3125 3510 3527 4979 5065 5077 5423 Size: 786 367 385 17 1452 86 12 346 Cuts at: 5423 1972 Size: 1977 Fragments arranged by size: 1977 1452 786 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4876 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4689 Fragments arranged by size: 4689 683 56 BtsI GCAGTG_nn' Cuts at: 2069 2944 3738 3758 2069 Size: 875 794 20 3739 Fragments arranged by size: 3739 875 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5428 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5393 DraI TTT'AAA Cuts at: 2732 2732 Size: 5428 DraIII CAC_nnn'GTG Cuts at: 2518 2518 Size: 5428 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4435 809 Size: 507 3119 1802 Fragments arranged by size: 3119 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2035 2875 3764 Size: 54 1425 50 275 18 205 840 889 Cuts at: 3764 5393 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 840 275 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2035 2875 1537 Size: 275 223 840 4090 Fragments arranged by size: 4090 840 275 223 EarI CTCTTCn'nnn_ Cuts at: 1353 3358 1353 Size: 2005 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2113 2528 4015 4389 4535 387 Size: 1064 138 524 415 1487 374 146 1280 Fragments arranged by size: 1487 1280 1064 524 415 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2636 2703 3491 4088 4875 5143 1239 Size: 393 1004 67 788 597 787 268 1524 Fragments arranged by size: 1524 1004 788 787 597 393 268 67 EcoNI CCTnn'n_nnAGG Cuts at: 2027 2027 Size: 5428 EcoRI G'AATT_C Cuts at: 2737 3115 2737 Size: 378 5050 FspI TGC'GCA Cuts at: 3934 3934 Size: 5428 HaeII r_GCGC'y Cuts at: 1678 2086 2206 4575 1678 Size: 408 120 2369 2531 Fragments arranged by size: 2531 2369 408 120 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 2412 2444 4069 Size: 32 135 32 479 32 329 32 1625 Cuts at: 4069 4101 4143 4175 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1625 479 329 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2374 2772 3018 669 Size: 988 281 436 398 246 3079 Fragments arranged by size: 3079 988 436 398 281 246 HindIII A'AGCT_T Cuts at: 748 2468 748 Size: 1720 3708 HpaI GTT'AAC Cuts at: 3018 3018 Size: 5428 KasI G'GCGC_C Cuts at: 2202 2202 Size: 5428 KpnI G_GTAC'C Cuts at: 2753 2753 Size: 5428 MfeI C'AATT_G Cuts at: 3027 3027 Size: 5428 MluI A'CGCG_T Cuts at: 5304 5304 Size: 5428 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4542 4726 1305 Size: 3237 184 2007 Fragments arranged by size: 3237 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3606 Fragments arranged by size: 3606 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2454 3386 3745 3904 518 Size: 648 1288 932 359 159 2042 Fragments arranged by size: 2042 1288 932 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2558 2844 3521 4669 4914 1606 Size: 952 286 677 1148 245 2120 Fragments arranged by size: 2120 1148 952 677 286 245 NarI GG'CG_CC Cuts at: 2203 2203 Size: 5428 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4876 NdeI CA'TA_TG Cuts at: 387 387 Size: 5428 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5428 NotI GC'GGCC_GC Cuts at: 2035 2875 2035 Size: 840 4588 NspI r_CATG'y Cuts at: 1132 1229 2056 2179 2441 2786 4331 1132 Size: 97 827 123 262 345 1545 2229 Fragments arranged by size: 2229 1545 827 345 262 123 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5428 PciI A'CATG_T Cuts at: 4327 4327 Size: 5428 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5428 PmeI GTTT'AAAC Cuts at: 2732 2732 Size: 5428 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3497 3529 5010 5042 3497 Size: 32 1481 32 3883 Fragments arranged by size: 3883 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5428 PsiI TTA'TAA Cuts at: 2998 2998 Size: 5428 PstI C_TGCA'G Cuts at: 830 2199 2780 830 Size: 1369 581 3478 Fragments arranged by size: 3478 1369 581 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5428 PvuII CAG'CTG Cuts at: 2558 2558 Size: 5428 SalI G'TCGA_C Cuts at: 1936 2770 1936 Size: 834 4594 SbfI CC_TGCA'GG Cuts at: 2780 2780 Size: 5428 ScaI AGT'ACT Cuts at: 3676 5388 3676 Size: 1712 3716 SfcI C'TryA_G Cuts at: 826 1045 2195 2597 2776 3911 4592 4783 826 Size: 219 1150 402 179 1135 681 191 1471 Fragments arranged by size: 1471 1150 1135 681 402 219 191 179 SfoI GGC'GCC Cuts at: 2204 2204 Size: 5428 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5428 SmaI CCC'GGG Cuts at: 1872 2755 1872 Size: 883 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2150 2429 3532 4433 4695 820 Size: 197 926 207 279 1103 901 262 1553 Fragments arranged by size: 1553 1103 926 901 279 262 207 197 SnaBI TAC'GTA Cuts at: 493 3219 493 Size: 2726 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5428 SphI G_CATG'C Cuts at: 1132 2786 1132 Size: 1654 3774 SspI AAT'ATT Cuts at: 5 52 2191 3352 5 Size: 47 2139 1161 2081 Fragments arranged by size: 2139 2081 1161 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5428 StyI C'CwwG_G Cuts at: 513 1065 2169 2252 2367 2590 2867 513 Size: 552 1104 83 115 223 277 3074 Fragments arranged by size: 3074 1104 552 277 223 115 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2063 3455 3472 3625 3810 4255 325 Size: 1255 483 1392 17 153 185 445 1498 Fragments arranged by size: 1498 1392 1255 483 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3674 5386 96 Size: 274 80 33 51 1443 1697 1712 138 Fragments arranged by size: 1712 1697 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2105 2137 2343 2375 5388 5420 2105 Size: 32 206 32 3013 32 2113 Fragments arranged by size: 3013 2113 206 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5428 XbaI T'CTAG_A Cuts at: 2764 2764 Size: 5428 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5428 XmaI C'CCGG_G Cuts at: 1870 2753 1870 Size: 883 4545 XmnI GAAnn'nnTTC Cuts at: 3557 3557 Size: 5428 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3236 276 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI