(Linear) (Six-base) MAPSORT of: KIEE6259.seq Check: 5712 from: 1 to: 723 KIEE6259.orf With 182 enzymes: SgfI * March 16, 2015 11:28 .. AccI GT'mk_AC Cuts at: 0 591 723 Size: 591 132 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 631 698 723 Size: 631 67 25 Fragments arranged by size: 631 67 25 AlwNI CAG_nnn'CTG Cuts at: 0 553 723 Size: 553 170 BanI G'GyrC_C Cuts at: 0 197 723 Size: 197 526 BanII G_rGCy'C Cuts at: 0 17 160 529 723 Size: 17 143 369 194 Fragments arranged by size: 369 194 143 17 BbeI G_GCGC'C Cuts at: 0 201 723 Size: 201 522 BbsI GAAGACnn'nnnn_ Cuts at: 0 661 710 723 Size: 661 49 13 Fragments arranged by size: 661 49 13 BclI T'GATC_A Cuts at: 0 515 644 723 Size: 515 129 79 Fragments arranged by size: 515 129 79 BmrI ACTGGGnnnn_n' Cuts at: 0 532 723 Size: 532 191 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 708 723 Size: 708 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 409 723 Size: 130 279 314 Fragments arranged by size: 314 279 130 BsaI GGTCTCn'nnnn_ Cuts at: 0 34 723 Size: 34 689 BsaHI Gr'CG_yC Cuts at: 0 61 198 723 Size: 61 137 525 Fragments arranged by size: 525 137 61 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 46 76 100 130 584 614 723 Size: 46 30 24 30 454 30 109 Fragments arranged by size: 454 109 46 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 7 322 723 Size: 7 315 401 Fragments arranged by size: 401 315 7 BseYI C'CCAG_C Cuts at: 0 153 546 723 Size: 153 393 177 Fragments arranged by size: 393 177 153 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 118 723 Size: 118 605 BsiEI CG_ry'CG Cuts at: 0 33 723 Size: 33 690 BsiHKAI G_wGCw'C Cuts at: 0 509 723 Size: 509 214 Bsp1286I G_dGCh'C Cuts at: 0 17 160 509 529 723 Size: 17 143 349 20 194 Fragments arranged by size: 349 194 143 20 17 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 134 723 Size: 134 589 BsrFI r'CCGG_y Cuts at: 0 638 723 Size: 638 85 BstAPI GCAn_nnn'nTGC Cuts at: 0 96 723 Size: 96 627 BstXI CCAn_nnnn'nTGG Cuts at: 0 343 723 Size: 343 380 BtsI GCAGTG_nn' Cuts at: 0 64 723 Size: 64 659 DraIII CAC_nnn'GTG Cuts at: 0 513 723 Size: 513 210 EaeI y'GGCC_r Cuts at: 0 30 723 Size: 30 693 EagI C'GGCC_G Cuts at: 0 30 723 Size: 30 693 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 108 523 723 Size: 108 415 200 Fragments arranged by size: 415 200 108 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 631 698 723 Size: 631 67 25 Fragments arranged by size: 631 67 25 EcoNI CCTnn'n_nnAGG Cuts at: 0 22 723 Size: 22 701 HaeII r_GCGC'y Cuts at: 0 81 201 723 Size: 81 120 522 Fragments arranged by size: 522 120 81 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 46 78 407 439 723 Size: 46 32 329 32 284 Fragments arranged by size: 329 284 46 32 32 HincII GTy'rAC Cuts at: 0 369 723 Size: 369 354 HindIII A'AGCT_T Cuts at: 0 463 723 Size: 463 260 KasI G'GCGC_C Cuts at: 0 197 723 Size: 197 526 MslI CAynn'nnrTG Cuts at: 0 449 723 Size: 449 274 MspA1I CmG'CkG Cuts at: 0 553 723 Size: 553 170 NarI GG'CG_CC Cuts at: 0 198 723 Size: 198 525 NotI GC'GGCC_GC Cuts at: 0 30 723 Size: 30 693 NspI r_CATG'y Cuts at: 0 51 174 436 723 Size: 51 123 262 287 Fragments arranged by size: 287 262 123 51 PstI C_TGCA'G Cuts at: 0 194 723 Size: 194 529 PvuII CAG'CTG Cuts at: 0 553 723 Size: 553 170 SfcI C'TryA_G Cuts at: 0 190 592 723 Size: 190 402 131 Fragments arranged by size: 402 190 131 SfoI GGC'GCC Cuts at: 0 199 723 Size: 199 524 SmlI C'TyrA_G Cuts at: 0 145 424 723 Size: 145 279 299 Fragments arranged by size: 299 279 145 SspI AAT'ATT Cuts at: 0 186 723 Size: 186 537 StyI C'CwwG_G Cuts at: 0 164 247 362 585 723 Size: 164 83 115 223 138 Fragments arranged by size: 223 164 138 115 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 58 723 Size: 58 665 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 100 132 338 370 723 Size: 100 32 206 32 353 Fragments arranged by size: 353 206 100 32 32 Enzymes that do cut: AccI AcuI AlwNI BanI BanII BbeI BbsI BclI BmrI BplI BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BstAPI BstXI BtsI DraIII EaeI EagI EciI Eco57MI EcoNI HaeII Hin4I HincII HindIII KasI MslI MspA1I NarI NotI NspI PstI PvuII SfcI SfoI SmlI SspI StyI TaqII TstI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I BmgBI BmtI BpmI Bpu10I BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EarI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HpaI KpnI MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV NheI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI