################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MseI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI StyI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AciI 886 GCGG CCGC(-3/-1) AgeI 410 ACCGGT A^CCGGT AlwI 120 GATCC GGATC(4/5) AlwNI 118 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 488 GGTCC G^G[AT]CC BalI 638 TGGCCA TGG^CCA BanII 944 GGGCTC G[GA]GC[CT]^C BbvI 387 GCTGC GCAGC(8/12) BglI 496 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 255 AGATCT A^GATCT BsaWI 410 ACCGGT [AT]^CCGG[AT] Bsp1407I 373 TGTACA T^GTACA BspHI 452 TCATGA T^CATGA BsrFI 410 ACCGGT [GA]^CCGG[CT] BsrGI 373 TGTACA T^GTACA CfoI 385 GCGC GCG^C Cfr10I 410 ACCGGT [GA]^CCGG[CT] DraII 487 AGGTCCT [GA]G^G[ACGT]CC[CT] EarI 796 CTCTTC CTCTTC(1/4) EcoO109I 487 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRV 867 GATATC GAT^ATC Fnu4HI 387 GCTGC GC^[ACGT]GC HapII 411 CCGG C^CGG HhaI 385 GCGC GCG^C HinP1I 385 GCGC G^CGC HpaII 411 CCGG C^CGG ItaI 387 GCTGC GC^[ACGT]GC Ksp632I 796 CTCTTC CTCTTC(1/4) MaeIII 889 GTGAC ^GT[ACGT]AC MluNI 638 TGGCCA TGG^CCA MscI 638 TGGCCA TGG^CCA MspA1I 884 CAGCGG C[AC]G^C[GT]G MspI 411 CCGG C^CGG NspI 824 ACATGC [GA]CATG^[CT] PinAI 410 ACCGGT A^CCGGT PpuMI 487 AGGTCCT [GA]G^G[AT]CC[CT] RcaI 452 TCATGA T^CATGA SfaNI 729 GATGC GCATC(5/9) SspBI 373 TGTACA T^GTACA TseI 387 GCTGC G^C[AT]GC Tsp45I 889 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 374 GTAC GT^AC 438 GTAC BmyI 503 GGGCAC G[AGT]GC[ACT]^C 944 GGGCTC BsaJI 90 CCTGGG C^C[ACGT][ACGT]GG 491 CCTCGG BsmFI 555 GGGAC GGGAC(10/14) 280 GTCCC Bsp1286I 503 GGGCAC G[AGT]GC[ACT]^C 944 GGGCTC BstYI 119 AGATCC [GA]^GATC[CT] 255 AGATCT Cfr13I 488 GGTCC G^G[ACGT]CC 672 GGCCC DdeI 235 CTTAG C^T[ACGT]AG 949 CTCAG DpnII 120 GATC ^GATC 256 GATC EaeI 494 CGGCCA [CT]^GGCC[GA] 638 TGGCCA HphI 888 GGTGA GGTGA(8/7) 818 TCACC MboI 120 GATC ^GATC 256 GATC MflI 119 AGATCC [GA]^GATC[CT] 255 AGATCT NdeII 120 GATC ^GATC 256 GATC NlaIV 672 GGCCCC GG[ACGT]^[ACGT]CC 772 GGCTCC PleI 749 GACTC GAGTC(4/5) 891 GACTC RsaI 374 GTAC GT^AC 438 GTAC Sau3AI 120 GATC ^GATC 256 GATC Sau96I 488 GGTCC G^G[ACGT]CC 672 GGCCC SpeI 507 ACTAGT A^CTAGT 957 ACTAGT SspI 706 AATATT AAT^ATT 813 AATATT TfiI 202 GATTC G^A[AT]TC 687 GATTC Tsp509I 351 AATT ^AATT 912 AATT XcmI 641 CCATCTTTTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 726 CCAGATGCAGTCTGG XhoII 119 AGATCC [GA]^GATC[CT] 255 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AluI BfaI BsiYI BslI BsmAI BsrI BssKI BstNI Cac8I EcoRII HaeIII HinfI MaeI MboII MnlI MslI MvaI MwoI NlaIII ScrFI SmlI TspRI