################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT14I FauI FbaI FseI HaeII HapII HgaI Hin1I HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AfaI 326 GTAC GT^AC AocI 235 CCTCAGG CC^T[ACGT]AGG AvaII 446 GGACC G^G[AT]CC AviII 289 TGCGCA TGC^GCA BalI 590 TGGCCA TGG^CCA BbsI 789 GTCTTC GAAGAC(2/6) BciVI 646 GTATCC GTATCC(6/5) BfaI 244 CTAG C^TAG BpuAI 789 GTCTTC GAAGAC(2/6) BsaJI 504 CCGAGG C^C[ACGT][ACGT]GG BseRI 619 CTCCTC GAGGAG(10/8) BsgI 545 CTGCAC GTGCAG(16/14) BsmFI 45 GGGAC GGGAC(10/14) BspHI 404 TCATGA T^CATGA BspMI 251 ACCTGC ACCTGC(4/8) Bsu36I 235 CCTCAGG CC^T[ACGT]AGG Cac8I 429 GCTGGC GC[ACGT]^[ACGT]GC DraII 539 AGGCCCC [GA]G^G[ACGT]CC[CT] DrdI 780 GACACCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 235 CCTCAGG CC^T[ACGT]AGG EcoO109I 539 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 31 GAATTC G^AATTC EcoT22I 578 ATGCAT ATGCA^T FspI 289 TGCGCA TGC^GCA MaeI 244 CTAG C^TAG MboII 790 TCTTC GAAGA(8/7) MluNI 590 TGGCCA TGG^CCA MscI 590 TGGCCA TGG^CCA MseI 848 TTAA T^TAA NsiI 578 ATGCAT ATGCA^T PflMI 729 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 404 TCATGA T^CATGA RsaI 326 GTAC GT^AC SexAI 104 ACCTGGT A^CC[AT]GGT SfcI 861 CTATAG C^T[GA][CT]AG SmlI 703 CTCAAG C^T[CT][GA]AG SpeI 243 ACTAGT A^CTAGT TaqI 297 TCGA T^CGA Tru9I 848 TTAA T^TAA TthHB8I 297 TCGA T^CGA Van91I 729 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 449 CCAGTGGCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 100 GTCTAC GT^[AC][GT]AC 472 GTAGAC AcsI 31 GAATTC [GA]^AATT[CT] 915 GAATTT AlwI 439 GGATC GGATC(4/5) 757 GGATC ApoI 31 GAATTC [GA]^AATT[CT] 915 GAATTT BsmI 277 GCATTC GAATGC(1/-1) 455 GCATTC BsrDI 273 CATTGC GCAATG(2/0) 519 CATTGC BsrI 330 CCAGT ACTGG(1/-1) 449 CCAGT BssKI 105 CCTGG ^CC[ACGT]GG 410 CCTGG BstF5I 563 CATCC GGATG(2/0) 879 GGATG BstNI 105 CCTGG CC^[AT]GG 410 CCTGG CfoI 290 GCGC GCG^C 335 GCGC EaeI 590 TGGCCA [CT]^GGCC[GA] 654 TGGCCG EcoRII 105 CCTGG ^CC[AT]GG 410 CCTGG FokI 563 CATCC GGATG(9/13) 879 GGATG HhaI 290 GCGC GCG^C 335 GCGC HinP1I 290 GCGC G^CGC 335 GCGC HincII 151 GTTGAC GT[CT]^[GA]AC 687 GTCAAC HindII 151 GTTGAC GT[CT]^[GA]AC 687 GTCAAC MspA1I 73 CAGCTG C[AC]G^C[GT]G 427 CAGCTG MvaI 105 CCTGG CC^[AT]GG 410 CCTGG MwoI 337 GCCCTTCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 509 GCAGTAACAGC NlaIV 540 GGCCCC GG[ACGT]^[ACGT]CC 724 GGCTCC PvuII 73 CAGCTG CAG^CTG 427 CAGCTG ScrFI 105 CCTGG CC^[ACGT]GG 410 CCTGG SfaNI 562 GCATC GCATC(5/9) 580 GCATC TfiI 615 GATTC G^A[AT]TC 639 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AluI BbvI BsiYI BslI BsmAI Cfr13I DdeI DpnII Fnu4HI HaeIII HinfI ItaI MaeIII MboI MnlI MslI NdeII NlaIII PleI Sau3AI Sau96I TseI Tsp45I Tsp509I TspRI