################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbsI BcgI BclI BcnI BfrI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MroI MspA1I MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AciI 324 CCGC CCGC(-3/-1) AfaI 138 GTAC GT^AC AflIII 679 ACATGT A^C[GA][CT]GT AlwNI 894 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 763 CCTGAGG CC^T[ACGT]AGG Asp700I 483 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 209 GGACC G^G[AT]CC BalI 332 TGGCCA TGG^CCA BanI 469 GGCACC G^G[CT][GA]CC BciVI 902 GTATCC GTATCC(6/5) BglI 385 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 548 AGATCT A^GATCT BmyI 193 GTGCCC G[AGT]GC[ACT]^C BpmI 166 CTGGAG CTGGAG(16/14) BsaI 873 GGTCTC GGTCTC(1/5) Bsp1286I 193 GTGCCC G[AGT]GC[ACT]^C BspLU11I 679 ACATGT A^CATGT BsrDI 621 CATTGC GCAATG(2/0) BssSI 380 CTCGTG CACGAG(-5/-1) BstF5I 633 CATCC GGATG(2/0) Bsu36I 763 CCTGAGG CC^T[ACGT]AGG DsaI 311 CCATGG C^C[GA][CT]GG EaeI 332 TGGCCA [CT]^GGCC[GA] Eco81I 763 CCTGAGG CC^T[ACGT]AGG FokI 633 CATCC GGATG(9/13) HapII 741 CCGG C^CGG HpaII 741 CCGG C^CGG MluNI 332 TGGCCA TGG^CCA MscI 332 TGGCCA TGG^CCA MseI 724 TTAA T^TAA MspI 741 CCGG C^CGG NcoI 311 CCATGG C^CATGG NdeI 317 CATATG CA^TATG NspI 679 ACATGT [GA]CATG^[CT] PflMI 460 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 208 GGGACCC [GA]G^G[AT]CC[CT] PstI 339 CTGCAG CTGCA^G RsaI 138 GTAC GT^AC SapI 447 GCTCTTC GCTCTTC(1/4) SexAI 65 ACCTGGT A^CC[AT]GGT SmlI 637 CTCAAG C^T[CT][GA]AG Tru9I 724 TTAA T^TAA Tsp509I 757 AATT ^AATT TspRI 795 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 460 CCATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 483 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AlwI 585 GATCC GGATC(4/5) 642 GATCC BbvCI 861 CCTCAGC CCTCAGC(-5/-2) 513 GCTGAGG BsgI 433 GTGCAG GTGCAG(16/14) 527 GTGCAG BsmAI 703 GTCTC GTCTC(1/5) 874 GTCTC BsmFI 208 GGGAC GGGAC(10/14) 344 GTCCC BsmI 292 GAATGC GAATGC(1/-1) 855 GAATGC BspHI 365 TCATGA T^CATGA 581 TCATGA BsrI 390 ACTGG ACTGG(1/-1) 358 CCAGT BstEII 69 GGTCACC G^GT[ACGT]ACC 102 GGTTACC BstPI 69 GGTCACC G^GT[ACGT]ACC 102 GGTTACC BstYI 548 AGATCT [GA]^GATC[CT] 641 AGATCC Cac8I 216 GCTTGC GC[ACGT]^[ACGT]GC 593 GCTTGC Cfr13I 209 GGACC G^G[ACGT]CC 882 GGCCC DraII 208 GGGACCC [GA]G^G[ACGT]CC[CT] 881 AGGCCCT EarI 9 CTCTTC CTCTTC(1/4) 448 CTCTTC EcoO109I 208 GGGACCC [GA]G^G[ACGT]CC[CT] 881 AGGCCCT EcoO65I 69 GGTCACC G^GT[ACGT]ACC 102 GGTTACC EcoT14I 243 CCTTGG C^C[AT][AT]GG 311 CCATGG HinfI 378 GACTC G^A[ACGT]TC 804 GACTC Ksp632I 9 CTCTTC CTCTTC(1/4) 448 CTCTTC MflI 548 AGATCT [GA]^GATC[CT] 641 AGATCC PleI 378 GACTC GAGTC(4/5) 804 GACTC RcaI 365 TCATGA T^CATGA 581 TCATGA Sau96I 209 GGACC G^G[ACGT]CC 882 GGCCC StyI 243 CCTTGG C^C[AT][AT]GG 311 CCATGG Tsp45I 70 GTCAC ^GT[GC]AC 497 GTGAC XhoII 548 AGATCT [GA]^GATC[CT] 641 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AluI BbvI BfaI BsaJI BsiYI BslI BssKI BstNI BstXI DdeI DpnII EcoRII Fnu4HI HaeIII HphI ItaI MaeI MaeIII MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI SfcI TseI