(Circular) (Six-base) MAPSORT of: pFN21AE5376.seq Check: 2017 from: 1 to: 5614 pFN21AE5376 5614 bp With 182 enzymes: SgfI * December 8, 2014 13:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3424 278 Size: 53 83 186 936 1888 2468 Fragments arranged by size: 2468 1888 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2957 1937 Size: 1020 4594 Acc65I G'GTAC_C Cuts at: 2935 2935 Size: 5614 AclI AA'CG_TT Cuts at: 1563 3741 4114 1563 Size: 2178 373 3063 Fragments arranged by size: 3063 2178 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2440 3677 5061 5329 1632 Size: 808 1237 1384 268 1917 Fragments arranged by size: 1917 1384 1237 808 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5417 AflIII A'CryG_T Cuts at: 1203 2684 4513 5490 1203 Size: 1481 1829 977 1327 Fragments arranged by size: 1829 1481 1327 977 AgeI A'CCGG_T Cuts at: 4445 4445 Size: 5614 AhdI GACnn_n'nnGTC Cuts at: 4343 4343 Size: 5614 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5614 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2369 2798 1184 Size: 1185 429 4000 Fragments arranged by size: 4000 1185 429 AlwNI CAG_nnn'CTG Cuts at: 2904 3357 4929 2904 Size: 453 1572 3589 Fragments arranged by size: 3589 1572 453 ApaLI G'TGCA_C Cuts at: 3671 4827 3671 Size: 1156 4458 ApoI r'AATT_y Cuts at: 1493 2923 3149 3301 5410 1493 Size: 1430 226 152 2109 1697 Fragments arranged by size: 2109 1697 1430 226 152 AseI AT'TA_AT Cuts at: 160 1032 4168 160 Size: 872 3136 1606 Fragments arranged by size: 3136 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 AvaI C'yCGr_G Cuts at: 1870 1943 2939 1870 Size: 73 996 4545 Fragments arranged by size: 4545 996 73 BamHI G'GATC_C Cuts at: 2944 3311 2944 Size: 367 5247 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2474 2935 4390 618 Size: 325 217 627 687 461 1455 1842 Fragments arranged by size: 1842 1455 687 627 461 325 217 BanII G_rGCy'C Cuts at: 1391 2933 1391 Size: 1542 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4643 BbvCI CC'TCA_GC Cuts at: 2519 2867 2519 Size: 348 5266 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3805 3839 1903 Size: 34 1868 34 3678 Fragments arranged by size: 3678 1868 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2918 3506 4716 2918 Size: 588 1210 3816 Fragments arranged by size: 3816 1210 588 BclI T'GATC_A Cuts at: 1548 1548 Size: 5614 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2396 4225 136 Size: 107 122 71 631 605 724 1829 1525 Fragments arranged by size: 1829 1525 724 631 605 122 107 71 BglII A'GATC_T Cuts at: 2553 5609 2553 Size: 3056 2558 BlpI GC'TnA_GC Cuts at: 3031 3031 Size: 5614 Bme1580I G_kGCm'C Cuts at: 1790 2202 3675 4831 1790 Size: 412 1473 1156 2573 Fragments arranged by size: 2573 1473 1156 412 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5614 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2357 2906 3345 Size: 416 53 83 399 24 940 549 439 Cuts at: 3345 4303 442 Size: 958 1753 Fragments arranged by size: 1753 958 940 549 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5614 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 4274 1239 Size: 953 2082 2579 Fragments arranged by size: 2579 2082 953 Bpu10I CC'TnA_GC Cuts at: 2519 2867 2519 Size: 348 5266 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2627 3739 4604 4902 2627 Size: 1112 865 298 3339 Fragments arranged by size: 3339 1112 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2884 4277 882 Size: 617 1385 1393 2219 Fragments arranged by size: 2219 1393 1385 617 BsaAI yAC'GTr Cuts at: 493 1204 3405 493 Size: 711 2201 2702 Fragments arranged by size: 2702 2201 711 BsaBI GATnn'nnATC Cuts at: 5608 5608 Size: 5614 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3421 3803 4438 275 Size: 53 83 186 936 1888 382 635 1451 Fragments arranged by size: 1888 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4047 4445 4719 4866 5277 4047 Size: 398 274 147 411 4384 Fragments arranged by size: 4384 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5389 Fragments arranged by size: 5389 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5614 BseYI C'CCAG_C Cuts at: 2209 4817 5313 2209 Size: 2608 496 2510 Fragments arranged by size: 2608 2510 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2459 2553 1163 Size: 573 723 94 4224 Fragments arranged by size: 4224 723 573 94 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3064 3825 4853 1540 Size: 275 186 1063 761 1028 2301 Fragments arranged by size: 2301 1063 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3675 3760 4831 3675 Size: 85 1071 4458 Fragments arranged by size: 4458 1071 85 BsmI GAATG_Cn' Cuts at: 2303 2866 3125 3218 2303 Size: 563 259 93 4699 Fragments arranged by size: 4699 563 259 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2933 3675 3760 4831 1391 Size: 399 412 731 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 731 412 399 85 BspEI T'CCGG_A Cuts at: 5277 5277 Size: 5614 BspHI T'CATG_A Cuts at: 2370 2586 3503 5233 2370 Size: 216 917 1730 2751 Fragments arranged by size: 2751 1730 917 216 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2969 844 Size: 2125 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3501 1394 Size: 429 1678 3507 Fragments arranged by size: 3507 1678 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2625 4109 4283 65 Size: 1183 1377 1484 174 1396 Fragments arranged by size: 1484 1396 1377 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4258 4445 1700 Size: 110 2448 187 2869 Fragments arranged by size: 2869 2448 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5614 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5614 BssSI C'ACGA_G Cuts at: 2385 3674 4686 2385 Size: 1289 1012 3313 Fragments arranged by size: 3313 1289 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5614 BstBI TT'CG_AA Cuts at: 4418 4418 Size: 5614 BstEII G'GTnAC_C Cuts at: 1184 2074 2107 1184 Size: 890 33 4691 Fragments arranged by size: 4691 890 33 BstXI CCAn_nnnn'nTGG Cuts at: 2071 2209 2581 2071 Size: 138 372 5104 Fragments arranged by size: 5104 372 138 BstYI r'GATC_y Cuts at: 1972 2553 2646 2944 3311 3696 3713 5165 5251 Size: 581 93 298 367 385 17 1452 86 Cuts at: 5251 5263 5609 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 581 385 367 346 298 93 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2769 2769 Size: 5614 BtgI C'CryG_G Cuts at: 513 1065 2316 513 Size: 552 1251 3811 Fragments arranged by size: 3811 1251 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4875 Fragments arranged by size: 4875 683 56 BtsI GCAGTG_nn' Cuts at: 3130 3924 3944 3130 Size: 794 20 4800 Fragments arranged by size: 4800 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5614 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5579 DraI TTT'AAA Cuts at: 2918 2918 Size: 5614 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4621 809 Size: 507 3305 1802 Fragments arranged by size: 3305 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2337 3061 3950 Size: 54 1425 50 275 18 507 724 889 Cuts at: 3950 5579 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 724 507 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3061 1537 Size: 275 1249 4090 Fragments arranged by size: 4090 1249 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2020 2459 3544 1353 Size: 667 439 1085 3423 Fragments arranged by size: 3423 1085 667 439 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4201 4575 4721 387 Size: 1064 138 2612 374 146 1280 Fragments arranged by size: 2612 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2440 3677 4274 5061 5329 1239 Size: 393 560 248 1237 597 787 268 1524 Fragments arranged by size: 1524 1237 787 597 560 393 268 248 EcoO109I rG'GnC_Cy Cuts at: 2214 2887 2214 Size: 673 4941 EcoRI G'AATT_C Cuts at: 2923 3301 2923 Size: 378 5236 FspI TGC'GCA Cuts at: 4120 4120 Size: 5614 HaeII r_GCGC'y Cuts at: 1678 4761 1678 Size: 3083 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4255 4287 4329 4361 1373 Size: 32 135 32 2683 32 42 32 2626 Fragments arranged by size: 2683 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2958 3204 669 Size: 988 281 1020 246 3079 Fragments arranged by size: 3079 1020 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5614 HpaI GTT'AAC Cuts at: 3204 3204 Size: 5614 KpnI G_GTAC'C Cuts at: 2939 2939 Size: 5614 MfeI C'AATT_G Cuts at: 3213 3213 Size: 5614 MluI A'CGCG_T Cuts at: 5490 5490 Size: 5614 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2548 4728 4912 1305 Size: 1243 2180 184 2007 Fragments arranged by size: 2180 2007 1243 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2339 10 Size: 54 1425 343 507 3285 Fragments arranged by size: 3285 1425 507 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2081 2264 2579 2804 3572 3931 4090 Size: 648 915 183 315 225 768 359 159 Cuts at: 4090 518 Size: 2042 Fragments arranged by size: 2042 915 768 648 359 315 225 183 159 MspA1I CmG'CkG Cuts at: 1606 3030 3707 4855 5100 1606 Size: 1424 677 1148 245 2120 Fragments arranged by size: 2120 1424 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2316 513 Size: 552 1251 3811 Fragments arranged by size: 3811 1251 552 NdeI CA'TA_TG Cuts at: 387 2323 387 Size: 1936 3678 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5614 NotI GC'GGCC_GC Cuts at: 3061 3061 Size: 5614 NspI r_CATG'y Cuts at: 1132 1229 2688 2972 4517 1132 Size: 97 1459 284 1545 2229 Fragments arranged by size: 2229 1545 1459 284 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5614 PciI A'CATG_T Cuts at: 2684 4513 2684 Size: 1829 3785 PflMI CCAn_nnn'nTGG Cuts at: 1108 2471 1108 Size: 1363 4251 PfoI T'CCnGG_A Cuts at: 2169 2169 Size: 5614 PmeI GTTT'AAAC Cuts at: 2918 2918 Size: 5614 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3683 3715 5196 5228 3683 Size: 32 1481 32 4069 Fragments arranged by size: 4069 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2214 2214 Size: 5614 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5614 PsiI TTA'TAA Cuts at: 3184 3184 Size: 5614 PstI C_TGCA'G Cuts at: 830 2348 2966 830 Size: 1518 618 3478 Fragments arranged by size: 3478 1518 618 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5614 SalI G'TCGA_C Cuts at: 1936 2956 1936 Size: 1020 4594 SanDI GG'GwC_CC Cuts at: 2214 2214 Size: 5614 SapI GCTCTTCn'nnn_ Cuts at: 2459 2459 Size: 5614 SbfI CC_TGCA'GG Cuts at: 2966 2966 Size: 5614 ScaI AGT'ACT Cuts at: 3862 5574 3862 Size: 1712 3902 SexAI A'CCwGG_T Cuts at: 2070 2070 Size: 5614 SfcI C'TryA_G Cuts at: 826 1045 2344 2427 2833 2962 4097 4778 4969 Size: 219 1299 83 406 129 1135 681 191 Cuts at: 4969 826 Size: 1471 Fragments arranged by size: 1471 1299 1135 681 406 219 191 129 83 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5614 SmaI CCC'GGG Cuts at: 1872 2941 1872 Size: 1069 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2642 3718 4619 4881 820 Size: 197 926 699 1076 901 262 1553 Fragments arranged by size: 1553 1076 926 901 699 262 197 SnaBI TAC'GTA Cuts at: 493 3405 493 Size: 2912 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5614 SphI G_CATG'C Cuts at: 1132 2972 1132 Size: 1840 3774 SspI AAT'ATT Cuts at: 5 52 3538 5 Size: 47 3486 2081 Fragments arranged by size: 3486 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5614 StyI C'CwwG_G Cuts at: 513 1065 2248 2316 3053 513 Size: 552 1183 68 737 3074 Fragments arranged by size: 3074 1183 737 552 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3641 3658 3811 3996 4441 325 Size: 1255 2061 17 153 185 445 1498 Fragments arranged by size: 2061 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2142 3860 5572 Size: 274 80 33 51 1443 165 1718 1712 Cuts at: 5572 96 Size: 138 Fragments arranged by size: 1718 1712 1443 274 165 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5574 5606 5574 Size: 32 5582 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5614 XbaI T'CTAG_A Cuts at: 2950 2950 Size: 5614 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5614 XmaI C'CCGG_G Cuts at: 1870 2939 1870 Size: 1069 4545 XmnI GAAnn'nnTTC Cuts at: 2492 3743 2492 Size: 1251 4363 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3422 276 Size: 53 83 186 936 1888 2468 Fragments arranged by size: 2468 1888 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SfiI SfoI SrfI SwaI XcmI