Restriction map for
pFN21AE6055
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AvaII AviII AvrII BamHI BanII
BbeI BbrPI BcgI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HpaI HpaII KasI KpnI KspI
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 376 GTAGAC GT^[AC][GT]AC
AcsI 604 AAATTT [GA]^AATT[CT]
AflIII 947 ACGTGT A^C[GA][CT]GT
Alw44I 887 GTGCAC G^TGCAC
AlwI 258 GATCC GGATC(4/5)
AlwNI 922 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 887 GTGCAC G^TGCAC
ApoI 604 AAATTT [GA]^AATT[CT]
AspI 935 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 136 GGTGCC G^G[CT][GA]CC
BbsI 439 GTCTTC GAAGAC(2/6)
BbvCI 693 GCTGAGG CCTCAGC(-5/-2)
BciVI 3 GGATAC GTATCC(6/5)
BfaI 622 CTAG C^TAG
BglII 317 AGATCT A^GATCT
BpuAI 439 GTCTTC GAAGAC(2/6)
BsaI 172 GAGACC GGTCTC(1/5)
BseRI 145 CTCCTC GAGGAG(10/8)
BsmI 74 GCATTC GAATGC(1/-1)
BspHI 78 TCATGA T^CATGA
BsrDI 715 GCAATG GCAATG(2/0)
BstXI 254 CCAAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 621 GCTAGC GC[ACGT]^[ACGT]GC
DraIII 685 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
HincII 480 GTTGAC GT[CT]^[GA]AC
HindII 480 GTTGAC GT[CT]^[GA]AC
HindIII 431 AAGCTT A^AGCTT
HphI 158 TCACC GGTGA(8/7)
MaeI 622 CTAG C^TAG
MaeII 947 ACGT A^CGT
MseI 839 TTAA T^TAA
NheI 621 GCTAGC G^CTAGC
NlaIV 136 GGTGCC GG[ACGT]^[ACGT]CC
PflFI 935 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 78 TCATGA T^CATGA
TaqI 539 TCGA T^CGA
TfiI 816 GATTC G^A[AT]TC
Tru9I 839 TTAA T^TAA
Tsp509I 605 AATT ^AATT
Tth111I 935 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 539 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 195 GTAC GT^AC
411 GTAC
BalI 236 TGGCCA TGG^CCA
263 TGGCCA
BpmI 30 CTCCAG CTGGAG(16/14)
37 CTCCAG
BsgI 847 CTGCAC GTGCAG(16/14)
866 CTGCAC
BsiYI 198 CCATTTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
266 CCATCTTCTGG
BslI 198 CCATTTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
266 CCATCTTCTGG
BstYI 257 AGATCC [GA]^GATC[CT]
317 AGATCT
DpnII 258 GATC ^GATC
318 GATC
EaeI 236 TGGCCA [CT]^GGCC[GA]
263 TGGCCA
EarI 404 CTCTTC CTCTTC(1/4)
763 CTCTTC
EcoT14I 284 CCAAGG C^C[AT][AT]GG
722 CCAAGG
Ksp632I 404 CTCTTC CTCTTC(1/4)
763 CTCTTC
MboI 258 GATC ^GATC
318 GATC
MflI 257 AGATCC [GA]^GATC[CT]
317 AGATCT
MluNI 236 TGGCCA TGG^CCA
263 TGGCCA
MscI 236 TGGCCA TGG^CCA
263 TGGCCA
NdeII 258 GATC ^GATC
318 GATC
PflMI 198 CCATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
266 CCATCTTCTGG
RsaI 195 GTAC GT^AC
411 GTAC
Sau3AI 258 GATC ^GATC
318 GATC
StyI 284 CCAAGG C^C[AT][AT]GG
722 CCAAGG
Tsp45I 424 GTCAC ^GT[GC]AC
793 GTCAC
Van91I 198 CCATTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
266 CCATCTTCTGG
XhoII 257 AGATCC [GA]^GATC[CT]
317 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI AspHI BbvI BmyI BsaJI
BsiHKAI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI DdeI EcoRII Fnu4HI
FokI HaeIII HinfI ItaI MaeIII
MboII MnlI MslI MvaI MwoI
NlaIII PleI ScrFI SfaNI TseI
TspRI