Restriction map for
pFN21AE6055
(Circular) (Six-base) MAPSORT of: pFN21AE6055.seq Check: 6246 from: 1 to: 5662
pFN21AE6055 5662 bp
With 182 enzymes: SgfI *
December 8, 2014 13:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3472 278
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2382 3005 1937
Size: 445 623 4594
Fragments arranged by size:
4594 623 445
Acc65I G'GTAC_C
Cuts at: 2983 2983
Size: 5662
AclI AA'CG_TT
Cuts at: 1563 3789 4162 1563
Size: 2226 373 3063
Fragments arranged by size:
3063 2226 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2396 3725 5109 5377 1632
Size: 764 1329 1384 268 1917
Fragments arranged by size:
1917 1384 1329 764 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5465
AflIII A'CryG_T
Cuts at: 1203 2952 4561 5538 1203
Size: 1749 1609 977 1327
Fragments arranged by size:
1749 1609 1327 977
AgeI A'CCGG_T
Cuts at: 4493 4493
Size: 5662
AhdI GACnn_n'nnGTC
Cuts at: 4391 4391
Size: 5662
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5662
AlwNI CAG_nnn'CTG
Cuts at: 2932 3405 4977 2932
Size: 473 1572 3617
Fragments arranged by size:
3617 1572 473
ApaLI G'TGCA_C
Cuts at: 2892 3719 4875 2892
Size: 827 1156 3679
Fragments arranged by size:
3679 1156 827
ApoI r'AATT_y
Cuts at: 1493 2609 2971 3197 3349 5458 1493
Size: 1116 362 226 152 2109 1697
Fragments arranged by size:
2109 1697 1116 362 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4216 160
Size: 872 3184 1606
Fragments arranged by size:
3184 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
AvaI C'yCGr_G
Cuts at: 1870 1943 2987 1870
Size: 73 1044 4545
Fragments arranged by size:
4545 1044 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2183 2216 2764 2797 2183
Size: 33 548 33 5048
Fragments arranged by size:
5048 548 33 33
BamHI G'GATC_C
Cuts at: 2992 3359 2992
Size: 367 5295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2141 2983 4438 618
Size: 325 217 627 354 842 1455 1842
Fragments arranged by size:
1842 1455 842 627 354 325 217
BanII G_rGCy'C
Cuts at: 1391 2981 1391
Size: 1590 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2437 928
Size: 971 538 4153
Fragments arranged by size:
4153 971 538
BbvCI CC'TCA_GC
Cuts at: 2699 2699
Size: 5662
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3853 3887 1903
Size: 34 1916 34 3678
Fragments arranged by size:
3678 1916 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2002 3554 4764 2002
Size: 1552 1210 2900
Fragments arranged by size:
2900 1552 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5662
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4273 136
Size: 107 122 71 631 605 2601 1525
Fragments arranged by size:
2601 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2322 5657 2322
Size: 3335 2327
BlpI GC'TnA_GC
Cuts at: 3079 3079
Size: 5662
Bme1580I G_kGCm'C
Cuts at: 1790 2896 3723 4879 1790
Size: 1106 827 1156 2573
Fragments arranged by size:
2573 1156 1106 827
BmgBI CAC'GTC
Cuts at: 1318 2953 1318
Size: 1635 4027
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2047 2489 2927
Size: 416 53 83 399 24 630 442 438
Cuts at: 2927 2951 3393 4351 442
Size: 24 442 958 1753
Fragments arranged by size:
1753 958 630 442 442 438 416 399
83 53 24 24
BmtI G_CTAG'C
Cuts at: 1056 2630 1056
Size: 1574 4088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2020 2027 4322 1239
Size: 781 7 2295 2579
Fragments arranged by size:
2579 2295 781 7
Bpu10I CC'TnA_GC
Cuts at: 2471 2699 2471
Size: 228 5434
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3787 4652 4950 3787
Size: 865 298 4499
Fragments arranged by size:
4499 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2171 4325 882
Size: 617 672 2154 2219
Fragments arranged by size:
2219 2154 672 617
BsaAI yAC'GTr
Cuts at: 493 1204 3453 493
Size: 711 2249 2702
Fragments arranged by size:
2702 2249 711
BsaBI GATnn'nnATC
Cuts at: 5656 5656
Size: 5662
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3469 3851 4486 275
Size: 53 83 186 936 1936 382 635 1451
Fragments arranged by size:
1936 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4095 4493 4767 4914 5325 4095
Size: 398 274 147 411 4432
Fragments arranged by size:
4432 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2087 2117 1178
Size: 30 165 30 684 30 4723
Fragments arranged by size:
4723 684 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2141 1186
Size: 955 4707
BseYI C'CCAG_C
Cuts at: 2209 4865 5361 2209
Size: 2656 496 2510
Fragments arranged by size:
2656 2510 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2837 2856 1163
Size: 573 1101 19 3969
Fragments arranged by size:
3969 1101 573 19
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3112 3873 4901 1540
Size: 275 186 1111 761 1028 2301
Fragments arranged by size:
2301 1111 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2550 2741 2896 3723 3808 4879 2550
Size: 191 155 827 85 1071 3333
Fragments arranged by size:
3333 1071 827 191 155 85
BsmI GAATG_Cn'
Cuts at: 2079 3173 3266 2079
Size: 1094 93 4475
Fragments arranged by size:
4475 1094 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2550 2741 2896 2981 3723 3808 4879
Size: 399 760 191 155 85 742 85 1071
Cuts at: 4879 1391
Size: 2174
Fragments arranged by size:
2174 1071 760 742 399 191 155 85
85
BspEI T'CCGG_A
Cuts at: 5325 5325
Size: 5662
BspHI T'CATG_A
Cuts at: 2083 3551 5281 2083
Size: 1468 1730 2464
Fragments arranged by size:
2464 1730 1468
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3017 844
Size: 2173 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3549 1394
Size: 429 1726 3507
Fragments arranged by size:
3507 1726 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2727 4157 4331 65
Size: 1183 1479 1430 174 1396
Fragments arranged by size:
1479 1430 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4306 4493 1700
Size: 110 2496 187 2869
Fragments arranged by size:
2869 2496 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5662
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5662
BssSI C'ACGA_G
Cuts at: 3722 4734 3722
Size: 1012 4650
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5662
BstBI TT'CG_AA
Cuts at: 4466 4466
Size: 5662
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5662
BstXI CCAn_nnnn'nTGG
Cuts at: 2266 2266
Size: 5662
BstYI r'GATC_y
Cuts at: 1972 2262 2322 2992 3359 3744 3761 5213 5299
Size: 290 60 670 367 385 17 1452 86
Cuts at: 5299 5311 5657 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 670 385 367 346 290 86
60 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4171
Fragments arranged by size:
4171 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4923
Fragments arranged by size:
4923 683 56
BtsI GCAGTG_nn'
Cuts at: 2229 2335 2548 3178 3972 3992 2229
Size: 106 213 630 794 20 3899
Fragments arranged by size:
3899 794 630 213 106 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5627
DraI TTT'AAA
Cuts at: 2966 2966
Size: 5662
DraIII CAC_nnn'GTG
Cuts at: 2695 2695
Size: 5662
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4669 809
Size: 507 3353 1802
Fragments arranged by size:
3353 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 2268 3109
Size: 54 1425 50 275 18 411 27 841
Cuts at: 3109 3998 5627 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 841 411 275 54 50
43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3109 1537
Size: 275 1297 4090
Fragments arranged by size:
4090 1297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2415 2774 3592 1353
Size: 1062 359 818 3423
Fragments arranged by size:
3423 1062 818 359
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4249 4623 4769 387
Size: 1064 138 2660 374 146 1280
Fragments arranged by size:
2660 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2027 2396 3725 4322 5109 5377
Size: 393 388 7 369 1329 597 787 268
Cuts at: 5377 1239
Size: 1524
Fragments arranged by size:
1524 1329 787 597 393 388 369 268
7
EcoRI G'AATT_C
Cuts at: 2971 3349 2971
Size: 378 5284
FspI TGC'GCA
Cuts at: 4168 4168
Size: 5662
HaeII r_GCGC'y
Cuts at: 1678 4809 1678
Size: 3131 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4303 4335 4377 4409 1373
Size: 32 135 32 2731 32 42 32 2626
Fragments arranged by size:
2731 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2487 3006 3252 669
Size: 988 281 549 519 246 3079
Fragments arranged by size:
3079 988 549 519 281 246
HindIII A'AGCT_T
Cuts at: 748 2436 748
Size: 1688 3974
HpaI GTT'AAC
Cuts at: 3252 3252
Size: 5662
KpnI G_GTAC'C
Cuts at: 2987 2987
Size: 5662
MfeI C'AATT_G
Cuts at: 3261 3261
Size: 5662
MluI A'CGCG_T
Cuts at: 5538 5538
Size: 5662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4776 4960 1305
Size: 3471 184 2007
Fragments arranged by size:
3471 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2243 2270 10
Size: 54 1425 343 411 27 3402
Fragments arranged by size:
3402 1425 411 343 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2063 2252 2495 2600 3620 3979 4138
Size: 648 897 189 243 105 1020 359 159
Cuts at: 4138 518
Size: 2042
Fragments arranged by size:
2042 1020 897 648 359 243 189 159
105
MspA1I CmG'CkG
Cuts at: 1606 3078 3755 4903 5148 1606
Size: 1472 677 1148 245 2120
Fragments arranged by size:
2120 1472 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4171
Fragments arranged by size:
4171 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5662
NheI G'CTAG_C
Cuts at: 1052 2626 1052
Size: 1574 4088
NotI GC'GGCC_GC
Cuts at: 3109 3109
Size: 5662
NspI r_CATG'y
Cuts at: 1132 1229 3020 4565 1132
Size: 97 1791 1545 2229
Fragments arranged by size:
2229 1791 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5662
PciI A'CATG_T
Cuts at: 4561 4561
Size: 5662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2209 2277 1108
Size: 1101 68 4493
Fragments arranged by size:
4493 1101 68
PfoI T'CCnGG_A
Cuts at: 2531 2531
Size: 5662
PmeI GTTT'AAAC
Cuts at: 2966 2966
Size: 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3731 3763 5244 5276 3731
Size: 32 1481 32 4117
Fragments arranged by size:
4117 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5662
PsiI TTA'TAA
Cuts at: 3232 3232
Size: 5662
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2597 2629 2597
Size: 32 5630
PstI C_TGCA'G
Cuts at: 830 3014 830
Size: 2184 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5662
SalI G'TCGA_C
Cuts at: 1936 3004 1936
Size: 1068 4594
SbfI CC_TGCA'GG
Cuts at: 3014 3014
Size: 5662
ScaI AGT'ACT
Cuts at: 3910 5622 3910
Size: 1712 3950
SfcI C'TryA_G
Cuts at: 826 1045 3010 4145 4826 5017 826
Size: 219 1965 1135 681 191 1471
Fragments arranged by size:
1965 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5662
SmaI CCC'GGG
Cuts at: 1872 2989 1872
Size: 1117 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3766 4667 4929 820
Size: 197 926 1823 901 262 1553
Fragments arranged by size:
1823 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3453 493
Size: 2960 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5662
SphI G_CATG'C
Cuts at: 1132 3020 1132
Size: 1888 3774
SspI AAT'ATT
Cuts at: 5 52 3586 5
Size: 47 3534 2081
Fragments arranged by size:
3534 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5662
StyI C'CwwG_G
Cuts at: 513 1065 2004 2289 2727 2958 3101 513
Size: 552 939 285 438 231 143 3074
Fragments arranged by size:
3074 939 552 438 285 231 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3689 3706 3859 4044 4489 325
Size: 1255 2109 17 153 185 445 1498
Fragments arranged by size:
2109 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3908 5620 96
Size: 274 80 33 51 1443 1931 1712 138
Fragments arranged by size:
1931 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2087 2119 2513 2545 5622 5654 2087
Size: 32 394 32 3077 32 2095
Fragments arranged by size:
3077 2095 394 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2943 1316
Size: 1627 4035
XbaI T'CTAG_A
Cuts at: 2998 2998
Size: 5662
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5662
XmaI C'CCGG_G
Cuts at: 1870 2987 1870
Size: 1117 4545
XmnI GAAnn'nnTTC
Cuts at: 3791 3791
Size: 5662
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3470 276
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI