################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BamHI BanI BbeI BbsI BcgI BciVI BfrI BglI BglII BlnI BpuAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I CfoI Cfr10I ClaI CpoI DraIII EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MflI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I Tth111I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AflIII 883 ACGTGT A^C[GA][CT]GT AhdI 427 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 845 GGATC GGATC(4/5) AocI 429 CCTCAGG CC^T[ACGT]AGG AspEI 427 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 405 GGTCC G^G[AT]CC BanII 786 GGGCTC G[GA]GC[CT]^C BbrPI 882 CACGTG CAC^GTG BbvCI 255 CCTCAGC CCTCAGC(-5/-2) BbvI 234 GCAGC GCAGC(8/12) BcnI 614 CCCGG CC^[GC]GG BlpI 351 GCTCAGC GC^T[ACGT]AGC Bpu1102I 351 GCTCAGC GC^T[ACGT]AGC BsaAI 882 CACGTG [CT]AC^GT[GA] BsaI 129 GGTCTC GGTCTC(1/5) BsmI 618 GAATGC GAATGC(1/-1) BspHI 473 TCATGA T^CATGA BsrDI 170 GCAATG GCAATG(2/0) Bsu36I 429 CCTCAGG CC^T[ACGT]AGG CelII 351 GCTCAGC GC^T[ACGT]AGC DraI 15 TTTAAA TTT^AAA DrdI 489 GACCATGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 427 GACCTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 429 CCTCAGG CC^T[ACGT]AGG Fnu4HI 234 GCAGC GC^[ACGT]GC HapII 615 CCGG C^CGG HpaII 615 CCGG C^CGG ItaI 234 GCAGC GC^[ACGT]GC MaeIII 704 GTAAC ^GT[ACGT]AC MspA1I 313 CAGCTG C[AC]G^C[GT]G MspI 615 CCGG C^CGG NciI 614 CCCGG CC^[GC]GG NlaIV 889 GGTTCC GG[ACGT]^[ACGT]CC PmaCI 882 CACGTG CAC^GTG PmlI 882 CACGTG CAC^GTG PpuMI 404 AGGTCCT [GA]G^G[AT]CC[CT] PstI 699 CTGCAG CTGCA^G PvuII 313 CAGCTG CAG^CTG RcaI 473 TCATGA T^CATGA SfcI 699 CTGCAG C^T[GA][CT]AG SmlI 876 CTTGAG C^T[CT][GA]AG TaqI 436 TCGA T^CGA TfiI 1070 GAATC G^A[AT]TC TseI 234 GCAGC G^C[AT]GC TthHB8I 436 TCGA T^CGA XbaI 73 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 196 GAATTC [GA]^AATT[CT] 965 GAATTC ApoI 196 GAATTC [GA]^AATT[CT] 965 GAATTC AspHI 379 GTGCTC G[AT]GC[AT]^C 879 GAGCAC BclI 140 TGATCA T^GATCA 302 TGATCA BfaI 74 CTAG C^TAG 590 CTAG BsaBI 303 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 465 GATGTTCATC BseRI 202 CTCCTC GAGGAG(10/8) 358 CTCCTC BsiHKAI 379 GTGCTC G[AT]GC[AT]^C 879 GAGCAC BsmFI 30 GGGAC GGGAC(10/14) 979 GTCCC BspMI 347 ACCTGC ACCTGC(4/8) 1079 ACCTGC BstXI 308 CCATCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 401 CCAAGGTCCTGG DraII 41 AGGGCCT [GA]G^G[ACGT]CC[CT] 404 AGGTCCT EcoO109I 41 AGGGCCT [GA]G^G[ACGT]CC[CT] 404 AGGTCCT EcoRI 196 GAATTC G^AATTC 965 GAATTC FbaI 140 TGATCA T^GATCA 302 TGATCA HphI 305 TCACC GGTGA(8/7) 389 TCACC MaeI 74 CTAG C^TAG 590 CTAG MamI 303 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 465 GATGTTCATC MseI 16 TTAA T^TAA 116 TTAA MslI 504 CACGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 510 CATGGTCATG NcoI 278 CCATGG C^CATGG 491 CCATGG SfaNI 160 GCATC GCATC(5/9) 695 GCATC StuI 320 AGGCCT AGG^CCT 872 AGGCCT Tru9I 16 TTAA T^TAA 116 TTAA Tsp509I 197 AATT ^AATT 966 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AfaI AluI AlwNI BalI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FokI HaeIII HinfI Ksp632I MaeII MboI MboII MluNI MnlI MscI MvaI MwoI NdeII NlaIII PflMI PleI RsaI Sau3AI Sau96I ScrFI StyI TspRI Van91I