(Circular) (Six-base) MAPSORT of: pFN21AE5584.seq Check: 5360 from: 1 to: 5800 pFN21AE5584 5800 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3610 278 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3143 1937 Size: 1206 4594 Acc65I G'GTAC_C Cuts at: 3121 3121 Size: 5800 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2905 3110 3863 5247 5515 1632 Size: 1273 205 753 1384 268 1917 Fragments arranged by size: 1917 1384 1273 753 268 205 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5603 AflIII A'CryG_T Cuts at: 1203 2888 4699 5676 1203 Size: 1685 1811 977 1327 Fragments arranged by size: 1811 1685 1327 977 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 2437 4529 2437 Size: 2092 3708 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5800 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2948 1184 Size: 1764 4036 AlwNI CAG_nnn'CTG Cuts at: 2198 2344 2673 3543 5115 2198 Size: 146 329 870 1572 2883 Fragments arranged by size: 2883 1572 870 329 146 ApaLI G'TGCA_C Cuts at: 3857 5013 3857 Size: 1156 4644 ApoI r'AATT_y Cuts at: 1493 2201 2970 3109 3335 3487 5596 1493 Size: 708 769 139 226 152 2109 1697 Fragments arranged by size: 2109 1697 769 708 226 152 139 AseI AT'TA_AT Cuts at: 160 1032 4354 160 Size: 872 3322 1606 Fragments arranged by size: 3322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 3125 1870 Size: 73 1182 4545 Fragments arranged by size: 4545 1182 73 BamHI G'GATC_C Cuts at: 3130 3497 3130 Size: 367 5433 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3121 4576 618 Size: 325 217 627 1334 1455 1842 Fragments arranged by size: 1842 1455 1334 627 325 217 BanII G_rGCy'C Cuts at: 1391 2795 3119 1391 Size: 1404 324 4072 Fragments arranged by size: 4072 1404 324 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4829 BbvCI CC'TCA_GC Cuts at: 2261 2261 Size: 5800 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3991 4025 1903 Size: 34 2054 34 3678 Fragments arranged by size: 3678 2054 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3692 4902 3692 Size: 1210 4590 BclI T'GATC_A Cuts at: 1548 2145 2307 1548 Size: 597 162 5041 Fragments arranged by size: 5041 597 162 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4411 136 Size: 107 122 71 631 605 2739 1525 Fragments arranged by size: 2739 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5795 5795 Size: 5800 BlpI GC'TnA_GC Cuts at: 2357 3217 2357 Size: 860 4940 Bme1580I G_kGCm'C Cuts at: 1790 3861 5017 1790 Size: 2071 1156 2573 Fragments arranged by size: 2573 2071 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5800 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2140 2186 3531 Size: 416 53 83 399 24 723 46 1345 Cuts at: 3531 4489 442 Size: 958 1753 Fragments arranged by size: 1753 1345 958 723 416 399 83 53 46 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5800 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2991 2991 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2122 2342 2450 2961 4460 1239 Size: 883 220 108 511 1499 2579 Fragments arranged by size: 2579 1499 883 511 220 108 Bpu10I CC'TnA_GC Cuts at: 2261 2846 2261 Size: 585 5215 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2902 3925 4790 5088 2902 Size: 1023 865 298 3614 Fragments arranged by size: 3614 1023 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2140 4463 882 Size: 617 641 2323 2219 Fragments arranged by size: 2323 2219 641 617 BsaAI yAC'GTr Cuts at: 493 1204 2889 3591 493 Size: 711 1685 702 2702 Fragments arranged by size: 2702 1685 711 702 BsaBI GATnn'nnATC Cuts at: 2312 2474 5794 2312 Size: 162 3320 2318 Fragments arranged by size: 3320 2318 162 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3607 3989 4624 275 Size: 53 83 186 936 2074 382 635 1451 Fragments arranged by size: 2074 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4233 4631 4905 5052 5463 4233 Size: 398 274 147 411 4570 Fragments arranged by size: 4570 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2120 2150 1178 Size: 30 165 30 717 30 4828 Fragments arranged by size: 4828 717 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2198 2354 1186 Size: 1012 156 4632 Fragments arranged by size: 4632 1012 156 BseYI C'CCAG_C Cuts at: 2171 2452 3022 5003 5499 2171 Size: 281 570 1981 496 2472 Fragments arranged by size: 2472 1981 570 496 281 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2318 2491 2697 2990 1163 Size: 573 582 173 206 293 3973 Fragments arranged by size: 3973 582 573 293 206 173 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3250 4011 5039 1540 Size: 275 186 1249 761 1028 2301 Fragments arranged by size: 2301 1249 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2388 2888 3861 3946 5017 2388 Size: 500 973 85 1071 3171 Fragments arranged by size: 3171 1071 973 500 85 BsmI GAATG_Cn' Cuts at: 2629 3311 3404 2629 Size: 682 93 5025 Fragments arranged by size: 5025 682 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2388 2795 2888 3119 3861 3946 5017 Size: 399 598 407 93 231 742 85 1071 Cuts at: 5017 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 598 407 399 231 93 85 BspEI T'CCGG_A Cuts at: 5463 5463 Size: 5800 BspHI T'CATG_A Cuts at: 2478 3689 5419 2478 Size: 1211 1730 2859 Fragments arranged by size: 2859 1730 1211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2361 3093 3155 844 Size: 1517 732 62 3489 Fragments arranged by size: 3489 1517 732 62 BsrBI CCG'CTC Cuts at: 1394 1823 3687 1394 Size: 429 1864 3507 Fragments arranged by size: 3507 1864 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2182 4295 4469 65 Size: 1183 934 2113 174 1396 Fragments arranged by size: 2113 1396 1183 934 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4444 4631 1700 Size: 110 2634 187 2869 Fragments arranged by size: 2869 2634 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5800 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5800 BssSI C'ACGA_G Cuts at: 3860 4872 3860 Size: 1012 4788 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5800 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5800 BstXI CCAn_nnnn'nTGG Cuts at: 2320 2413 2320 Size: 93 5707 BstYI r'GATC_y Cuts at: 1972 3130 3497 3882 3899 5351 5437 5449 5795 Size: 1158 367 385 17 1452 86 12 346 Cuts at: 5795 1972 Size: 1977 Fragments arranged by size: 1977 1452 1158 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2435 2435 Size: 5800 BtgI C'CryG_G Cuts at: 513 1065 2283 2496 2601 513 Size: 552 1218 213 105 3712 Fragments arranged by size: 3712 1218 552 213 105 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5061 Fragments arranged by size: 5061 683 56 BtsI GCAGTG_nn' Cuts at: 2184 3316 4110 4130 2184 Size: 1132 794 20 3854 Fragments arranged by size: 3854 1132 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 2022 3104 2022 Size: 1082 4718 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2500 4807 809 Size: 507 1184 2307 1802 Fragments arranged by size: 2307 1802 1184 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2280 2415 2541 Size: 54 1425 50 275 18 450 135 126 Cuts at: 2541 2871 2958 3247 4136 5765 8 Size: 330 87 289 889 1629 43 Fragments arranged by size: 1629 1425 889 450 330 289 275 135 126 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3247 1537 Size: 275 1435 4090 Fragments arranged by size: 4090 1435 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2276 2643 2921 2960 3086 3730 1353 Size: 923 367 278 39 126 644 3423 Fragments arranged by size: 3423 923 644 367 278 126 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4387 4761 4907 387 Size: 1064 138 2798 374 146 1280 Fragments arranged by size: 2798 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2122 2342 2450 2905 2961 3110 3863 Size: 393 490 220 108 455 56 149 753 Cuts at: 3863 4460 5247 5515 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 753 597 490 455 393 268 220 149 108 56 EcoO109I rG'GnC_Cy Cuts at: 2047 2410 2047 Size: 363 5437 EcoRI G'AATT_C Cuts at: 2201 2970 3109 3487 2201 Size: 769 139 378 4514 Fragments arranged by size: 4514 769 378 139 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2861 2861 Size: 5800 FspI TGC'GCA Cuts at: 4306 4306 Size: 5800 HaeII r_GCGC'y Cuts at: 1678 4947 1678 Size: 3269 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2423 2455 4441 4473 4515 Size: 32 135 32 851 32 1986 32 42 Cuts at: 4515 4547 1373 Size: 32 2626 Fragments arranged by size: 2626 1986 851 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3144 3390 669 Size: 988 281 1206 246 3079 Fragments arranged by size: 3079 1206 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5800 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KpnI G_GTAC'C Cuts at: 3125 3125 Size: 5800 MfeI C'AATT_G Cuts at: 3399 3399 Size: 5800 MluI A'CGCG_T Cuts at: 5676 5676 Size: 5800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4914 5098 1305 Size: 3609 184 2007 Fragments arranged by size: 3609 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2282 2417 2543 2873 2960 Size: 54 1425 343 450 135 126 330 87 Cuts at: 2960 10 Size: 2850 Fragments arranged by size: 2850 1425 450 343 330 135 126 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 2513 2519 3758 4117 4276 518 Size: 648 1347 6 1239 359 159 2042 Fragments arranged by size: 2042 1347 1239 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2320 3216 3893 5041 5286 1606 Size: 714 896 677 1148 245 2120 Fragments arranged by size: 2120 1148 896 714 677 245 NcoI C'CATG_G Cuts at: 513 1065 2283 2496 513 Size: 552 1218 213 3817 Fragments arranged by size: 3817 1218 552 213 NdeI CA'TA_TG Cuts at: 387 387 Size: 5800 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5800 NotI GC'GGCC_GC Cuts at: 3247 3247 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 3158 4703 1132 Size: 97 1929 1545 2229 Fragments arranged by size: 2229 1929 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5800 PciI A'CATG_T Cuts at: 4699 4699 Size: 5800 PflMI CCAn_nnn'nTGG Cuts at: 1108 2424 2590 2770 1108 Size: 1316 166 180 4138 Fragments arranged by size: 4138 1316 180 166 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PmlI CAC'GTG Cuts at: 2889 2889 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3869 3901 5382 5414 3869 Size: 32 1481 32 4255 Fragments arranged by size: 4255 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2410 2410 Size: 5800 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5800 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PstI C_TGCA'G Cuts at: 830 2708 3152 830 Size: 1878 444 3478 Fragments arranged by size: 3478 1878 444 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 PvuII CAG'CTG Cuts at: 2320 2320 Size: 5800 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 4048 5760 4048 Size: 1712 4088 SfcI C'TryA_G Cuts at: 826 1045 2704 3148 4283 4964 5155 826 Size: 219 1659 444 1135 681 191 1471 Fragments arranged by size: 1659 1471 1135 681 444 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 3127 1872 Size: 1255 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2881 3904 4805 5067 820 Size: 197 926 938 1023 901 262 1553 Fragments arranged by size: 1553 1023 938 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3591 493 Size: 3098 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 3158 1132 Size: 2026 3774 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 2327 2879 1517 Size: 810 552 4438 Fragments arranged by size: 4438 810 552 StyI C'CwwG_G Cuts at: 513 1065 2283 2406 2496 2874 3057 3239 513 Size: 552 1218 123 90 378 183 182 3074 Fragments arranged by size: 3074 1218 552 378 183 182 123 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3827 3844 3997 4182 4627 325 Size: 1255 2247 17 153 185 445 1498 Fragments arranged by size: 2247 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2085 2102 2346 Size: 274 80 33 51 1443 108 17 244 Cuts at: 2346 4046 5758 96 Size: 1700 1712 138 Fragments arranged by size: 1712 1700 1443 274 244 138 108 80 51 33 17 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2120 2152 5760 5792 2120 Size: 32 3608 32 2128 Fragments arranged by size: 3608 2128 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5800 XbaI T'CTAG_A Cuts at: 2078 3136 2078 Size: 1058 4742 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5800 XmaI C'CCGG_G Cuts at: 1870 3125 1870 Size: 1255 4545 XmnI GAAnn'nnTTC Cuts at: 3929 3929 Size: 5800 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3608 276 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI