################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AgeI AocI Aor51HI ApaI AscI AseI AsnI Asp700I AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BsrBI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FauI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MaeI MaeII MboII MluI MroI MspA1I MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### Acc65I 237 GGTACC G^GTACC AciI 369 CCGC CCGC(-3/-1) AcsI 922 AAATTT [GA]^AATT[CT] AflIII 737 ACATGT A^C[GA][CT]GT AhdI 544 GACCATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 16 AGCT AG^CT Alw44I 225 GTGCAC G^TGCAC AlwI 83 GGATC GGATC(4/5) AlwNI 720 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 225 GTGCAC G^TGCAC ApoI 922 AAATTT [GA]^AATT[CT] Asp718I 237 GGTACC G^GTACC AspEI 544 GACCATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 366 GGACC G^G[AT]CC BanI 237 GGTACC G^G[CT][GA]CC BanII 136 GGGCTC G[GA]GC[CT]^C BbvI 766 GCTGC GCAGC(8/12) BciVI 565 GTATCC GTATCC(6/5) BclI 752 TGATCA T^GATCA BcnI 513 CCGGG CC^[GC]GG BglI 292 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 18 CTCCAG CTGGAG(16/14) BsaBI 465 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmAI 935 GAGAC GTCTC(1/5) BsmFI 8 GGGAC GGGAC(10/14) Bsp1407I 107 TGTACA T^GTACA BspLU11I 737 ACATGT A^CATGT BspMI 724 ACCTGC ACCTGC(4/8) BsrDI 114 CATTGC GCAATG(2/0) BsrFI 173 ACCGGC [GA]^CCGG[CT] BsrGI 107 TGTACA T^GTACA BstYI 83 GGATCT [GA]^GATC[CT] CfoI 498 GCGC GCG^C Cfr10I 173 ACCGGC [GA]^CCGG[CT] DraII 164 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 219 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 233 CCATGG C^C[GA][CT]GG Eam1105I 544 GACCATATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 449 CCTTCTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 164 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRV 634 GATATC GAT^ATC FbaI 752 TGATCA T^GATCA Fnu4HI 766 GCTGC GC^[ACGT]GC HaeII 497 AGCGCC [GA]GCGC^[CT] HhaI 498 GCGC GCG^C HinP1I 498 GCGC G^CGC ItaI 766 GCTGC GC^[ACGT]GC KpnI 237 GGTACC GGTAC^C MamI 465 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 875 CAATTG C^AATTG MflI 83 GGATCT [GA]^GATC[CT] MunI 875 CAATTG C^AATTG NciI 513 CCGGG CC^[GC]GG NcoI 233 CCATGG C^CATGG NlaIV 237 GGTACC GG[ACGT]^[ACGT]CC NspI 737 ACATGT [GA]CATG^[CT] SspBI 107 TGTACA T^GTACA TseI 766 GCTGC G^C[AT]GC TspRI 210 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 83 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AspHI 225 GTGCAC G[AT]GC[AT]^C 346 GTGCTC BseRI 487 CTCCTC GAGGAG(10/8) 160 GAGGAG BsgI 169 CTGCAC GTGCAG(16/14) 726 CTGCAC BsiHKAI 225 GTGCAC G[AT]GC[AT]^C 346 GTGCTC DpnII 84 GATC ^GATC 753 GATC HapII 174 CCGG C^CGG 513 CCGG HpaII 174 CCGG C^CGG 513 CCGG MboI 84 GATC ^GATC 753 GATC MseI 287 TTAA T^TAA 920 TTAA MspI 174 CCGG C^CGG 513 CCGG NdeI 319 CATATG CA^TATG 547 CATATG NdeII 84 GATC ^GATC 753 GATC PleI 337 GAGTC GAGTC(4/5) 887 GAGTC Sau3AI 84 GATC ^GATC 753 GATC SfaNI 465 GATGC GCATC(5/9) 700 GATGC SmlI 271 CTCAAG C^T[CT][GA]AG 453 CTTGAG TfiI 35 GATTC G^A[AT]TC 903 GATTC Tru9I 287 TTAA T^TAA 920 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AfaI BalI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I Cfr13I EaeI EcoRII EcoT14I FokI HaeIII HinfI HphI MaeIII MluNI MnlI MscI MslI MvaI MwoI NlaIII RsaI Sau96I ScrFI StyI Tsp45I Tsp509I