################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AgeI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspHI BsrBI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaII ItaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StyI SwaI TseI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AflII 931 CTTAAG C^TTAAG AflIII 548 ACATGT A^C[GA][CT]GT AhdI 544 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 83 GGATC GGATC(4/5) AlwNI 720 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 711 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 544 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 366 GGACC G^G[AT]CC BanI 16 GGCACC G^G[CT][GA]CC BbsI 187 GTCTTC GAAGAC(2/6) BfrI 931 CTTAAG C^TTAAG BpmI 29 CTCCAG CTGGAG(16/14) BpuAI 187 GTCTTC GAAGAC(2/6) BsgI 726 CTGCAC GTGCAG(16/14) BsmFI 801 GGGAC GGGAC(10/14) Bsp1407I 107 TGTACA T^GTACA BspLU11I 548 ACATGT A^CATGT BsrGI 107 TGTACA T^GTACA BstYI 83 GGATCT [GA]^GATC[CT] Cac8I 292 GCCTGC GC[ACGT]^[ACGT]GC DdeI 454 CTTAG C^T[ACGT]AG DpnII 84 GATC ^GATC DraI 286 TTTAAA TTT^AAA Eam1105I 544 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 250 CTCTTC CTCTTC(1/4) HincII 586 GTTAAC GT[CT]^[GA]AC HindII 586 GTTAAC GT[CT]^[GA]AC HpaI 586 GTTAAC GTT^AAC Ksp632I 250 CTCTTC CTCTTC(1/4) MaeII 521 ACGT A^CGT MaeIII 542 GTGAC ^GT[ACGT]AC MboI 84 GATC ^GATC MflI 83 GGATCT [GA]^GATC[CT] MwoI 292 GCCTGCCTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 84 GATC ^GATC NspI 548 ACATGT [GA]CATG^[CT] PflMI 369 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Sau3AI 84 GATC ^GATC SspBI 107 TGTACA T^GTACA TaqI 705 TCGA T^CGA TfiI 666 GATTC G^A[AT]TC Tsp45I 542 GTGAC ^GT[GC]AC TthHB8I 705 TCGA T^CGA Van91I 369 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 83 GGATCT [GA]^GATC[CT] XmnI 711 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AluI 33 AGCT AG^CT 435 AGCT Alw44I 225 GTGCAC G^TGCAC 316 GTGCAC ApaLI 225 GTGCAC G^TGCAC 316 GTGCAC BalI 377 TGGCCA TGG^CCA 557 TGGCCA BsaJI 57 CCCTGG C^C[ACGT][ACGT]GG 362 CCCTGG BspMI 724 ACCTGC ACCTGC(4/8) 900 GCAGGT BsrDI 114 CATTGC GCAATG(2/0) 426 CATTGC Cfr13I 360 GGCCC G^G[ACGT]CC 366 GGACC DraIII 370 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 492 CACCAAGTG EaeI 377 TGGCCA [CT]^GGCC[GA] 557 TGGCCA EcoRV 564 GATATC GAT^ATC 634 GATATC MluNI 377 TGGCCA TGG^CCA 557 TGGCCA MscI 377 TGGCCA TGG^CCA 557 TGGCCA NlaIV 16 GGCACC GG[ACGT]^[ACGT]CC 440 GGTTCC PleI 337 GAGTC GAGTC(4/5) 684 GAGTC Sau96I 360 GGCCC G^G[ACGT]CC 366 GGACC SmlI 271 CTCAAG C^T[CT][GA]AG 931 CTTAAG StuI 164 AGGCCT AGG^CCT 713 AGGCCT Tsp509I 418 AATT ^AATT 946 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AfaI AspHI BmyI BsiHKAI BsiYI BslI Bsp1286I BssKI BstF5I BstNI EcoRII FokI HaeIII HinfI HphI MboII MnlI MseI MslI MvaI NlaIII RsaI ScrFI SfaNI Tru9I TspRI