(Circular) (Six-base) MAPSORT of: pFN21AE6059.seq Check: 3099 from: 1 to: 5665 pFN21AE6059 5665 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2738 2738 Size: 5665 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3475 278 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3008 1937 Size: 1071 4594 Acc65I G'GTAC_C Cuts at: 2986 2986 Size: 5665 AclI AA'CG_TT Cuts at: 1563 3792 4165 1563 Size: 2229 373 3063 Fragments arranged by size: 3063 2229 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2707 3728 5112 5380 1632 Size: 1075 1021 1384 268 1917 Fragments arranged by size: 1917 1384 1075 1021 268 AflII C'TTAA_G Cuts at: 820 1017 2936 820 Size: 197 1919 3549 Fragments arranged by size: 3549 1919 197 AflIII A'CryG_T Cuts at: 1203 2553 4564 5541 1203 Size: 1350 2011 977 1327 Fragments arranged by size: 2011 1350 1327 977 AgeI A'CCGG_T Cuts at: 4496 4496 Size: 5665 AhdI GACnn_n'nnGTC Cuts at: 2554 4394 2554 Size: 1840 3825 AleI CACnn'nnGTG Cuts at: 1166 2219 2745 1166 Size: 1053 526 4086 Fragments arranged by size: 4086 1053 526 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5665 AlwNI CAG_nnn'CTG Cuts at: 2730 3408 4980 2730 Size: 678 1572 3415 Fragments arranged by size: 3415 1572 678 ApaLI G'TGCA_C Cuts at: 2230 2321 3722 4878 2230 Size: 91 1401 1156 3017 Fragments arranged by size: 3017 1401 1156 91 ApoI r'AATT_y Cuts at: 1493 2974 3200 3352 5461 1493 Size: 1481 226 152 2109 1697 Fragments arranged by size: 2109 1697 1481 226 152 AseI AT'TA_AT Cuts at: 160 1032 4219 160 Size: 872 3187 1606 Fragments arranged by size: 3187 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 AvaI C'yCGr_G Cuts at: 1870 1943 2990 1870 Size: 73 1047 4545 Fragments arranged by size: 4545 1047 73 BamHI G'GATC_C Cuts at: 2995 3362 2995 Size: 367 5298 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 2986 4441 618 Size: 325 217 627 234 965 1455 1842 Fragments arranged by size: 1842 1455 965 627 325 234 217 BanII G_rGCy'C Cuts at: 1391 2984 1391 Size: 1593 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2185 928 Size: 971 286 4408 Fragments arranged by size: 4408 971 286 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3856 3890 1903 Size: 34 1919 34 3678 Fragments arranged by size: 3678 1919 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3557 4767 3557 Size: 1210 4455 BclI T'GATC_A Cuts at: 1548 1548 Size: 5665 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4276 136 Size: 107 122 71 631 605 2604 1525 Fragments arranged by size: 2604 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5660 5660 Size: 5665 BlpI GC'TnA_GC Cuts at: 3082 3082 Size: 5665 Bme1580I G_kGCm'C Cuts at: 1790 2234 2325 3726 4882 1790 Size: 444 91 1401 1156 2573 Fragments arranged by size: 2573 1401 1156 444 91 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5665 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3396 4354 442 Size: 416 53 83 399 24 1979 958 1753 Fragments arranged by size: 1979 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5665 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2019 4325 1239 Size: 780 2306 2579 Fragments arranged by size: 2579 2306 780 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2261 3790 4655 4953 2261 Size: 1529 865 298 2973 Fragments arranged by size: 2973 1529 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4328 882 Size: 617 2829 2219 Fragments arranged by size: 2829 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3456 493 Size: 711 2252 2702 Fragments arranged by size: 2702 2252 711 BsaBI GATnn'nnATC Cuts at: 5659 5659 Size: 5665 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3472 3854 4489 275 Size: 53 83 186 936 1939 382 635 1451 Fragments arranged by size: 1939 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4098 4496 4770 4917 5328 4098 Size: 398 274 147 411 4435 Fragments arranged by size: 4435 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5440 Fragments arranged by size: 5440 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5665 BseYI C'CCAG_C Cuts at: 2441 4868 5364 2441 Size: 2427 496 2742 Fragments arranged by size: 2742 2427 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2716 1163 Size: 573 980 4112 Fragments arranged by size: 4112 980 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3115 3876 4904 1540 Size: 275 186 1114 761 1028 2301 Fragments arranged by size: 2301 1114 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2226 2234 2325 2355 3726 3811 4882 2226 Size: 8 91 30 1371 85 1071 3009 Fragments arranged by size: 3009 1371 1071 91 85 30 8 BsmI GAATG_Cn' Cuts at: 3176 3269 3176 Size: 93 5572 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2234 2325 2355 2984 3726 3811 Size: 399 436 8 91 30 629 742 85 Cuts at: 3811 4882 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 629 436 399 91 85 30 8 BspEI T'CCGG_A Cuts at: 5328 5328 Size: 5665 BspHI T'CATG_A Cuts at: 3554 5284 3554 Size: 1730 3935 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2738 2896 3020 844 Size: 1894 158 124 3489 Fragments arranged by size: 3489 1894 158 124 BsrBI CCG'CTC Cuts at: 1394 1823 3552 1394 Size: 429 1729 3507 Fragments arranged by size: 3507 1729 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2118 2430 4160 4334 65 Size: 1183 870 312 1730 174 1396 Fragments arranged by size: 1730 1396 1183 870 312 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4309 4496 1700 Size: 110 2499 187 2869 Fragments arranged by size: 2869 2499 187 110 BsrGI T'GTAC_A Cuts at: 96 2112 96 Size: 2016 3649 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5665 BssSI C'ACGA_G Cuts at: 3725 4737 3725 Size: 1012 4653 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5665 BstBI TT'CG_AA Cuts at: 4469 4469 Size: 5665 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5665 BstYI r'GATC_y Cuts at: 1972 2088 2995 3362 3747 3764 5216 5302 5314 Size: 116 907 367 385 17 1452 86 12 Cuts at: 5314 5660 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 907 385 367 346 116 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5113 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4926 Fragments arranged by size: 4926 683 56 BtsI GCAGTG_nn' Cuts at: 2733 3181 3975 3995 2733 Size: 448 794 20 4403 Fragments arranged by size: 4403 794 448 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5665 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5630 DraI TTT'AAA Cuts at: 2293 2969 2293 Size: 676 4989 DraIII CAC_nnn'GTG Cuts at: 2380 2502 2380 Size: 122 5543 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4672 809 Size: 507 3356 1802 Fragments arranged by size: 3356 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2382 2562 3112 Size: 54 1425 50 275 18 552 180 550 Cuts at: 3112 4001 5630 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 552 550 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3112 1537 Size: 275 1300 4090 Fragments arranged by size: 4090 1300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2261 3595 1353 Size: 908 1334 3423 Fragments arranged by size: 3423 1334 908 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4252 4626 4772 387 Size: 1064 138 2663 374 146 1280 Fragments arranged by size: 2663 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2019 2707 3728 4325 5112 5380 1239 Size: 393 387 688 1021 597 787 268 1524 Fragments arranged by size: 1524 1021 787 688 597 393 387 268 EcoRI G'AATT_C Cuts at: 2974 3352 2974 Size: 378 5287 EcoRV GAT'ATC Cuts at: 2571 2641 2571 Size: 70 5595 FspI TGC'GCA Cuts at: 4171 4171 Size: 5665 HaeII r_GCGC'y Cuts at: 1678 4812 1678 Size: 3134 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2540 2572 2904 2936 4306 Size: 32 135 32 968 32 332 32 1370 Cuts at: 4306 4338 4380 4412 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1370 968 332 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2593 3009 3255 669 Size: 988 281 655 416 246 3079 Fragments arranged by size: 3079 988 655 416 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5665 HpaI GTT'AAC Cuts at: 2593 3255 2593 Size: 662 5003 KpnI G_GTAC'C Cuts at: 2990 2990 Size: 5665 MfeI C'AATT_G Cuts at: 3264 3264 Size: 5665 MluI A'CGCG_T Cuts at: 5541 5541 Size: 5665 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4779 4963 1305 Size: 3474 184 2007 Fragments arranged by size: 3474 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2384 2564 10 Size: 54 1425 343 552 180 3111 Fragments arranged by size: 3111 1425 552 343 180 54 MslI CAynn'nnrTG Cuts at: 518 1166 2219 2336 2360 2558 2745 3623 3982 Size: 648 1053 117 24 198 187 878 359 Cuts at: 3982 4141 518 Size: 159 2042 Fragments arranged by size: 2042 1053 878 648 359 198 187 159 117 24 MspA1I CmG'CkG Cuts at: 1606 3081 3758 4906 5151 1606 Size: 1475 677 1148 245 2120 Fragments arranged by size: 2120 1475 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5113 NdeI CA'TA_TG Cuts at: 387 387 Size: 5665 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5665 NotI GC'GGCC_GC Cuts at: 3112 3112 Size: 5665 NspI r_CATG'y Cuts at: 1132 1229 2557 3023 4568 1132 Size: 97 1328 466 1545 2229 Fragments arranged by size: 2229 1545 1328 466 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5665 PciI A'CATG_T Cuts at: 2553 4564 2553 Size: 2011 3654 PflMI CCAn_nnn'nTGG Cuts at: 1108 2380 1108 Size: 1272 4393 PmeI GTTT'AAAC Cuts at: 2969 2969 Size: 5665 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3734 3766 5247 5279 3734 Size: 32 1481 32 4120 Fragments arranged by size: 4120 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5665 PsiI TTA'TAA Cuts at: 3235 3235 Size: 5665 PstI C_TGCA'G Cuts at: 830 3017 830 Size: 2187 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5665 SalI G'TCGA_C Cuts at: 1936 3007 1936 Size: 1071 4594 SbfI CC_TGCA'GG Cuts at: 3017 3017 Size: 5665 ScaI AGT'ACT Cuts at: 3913 5625 3913 Size: 1712 3953 SfcI C'TryA_G Cuts at: 826 1045 3013 4148 4829 5020 826 Size: 219 1968 1135 681 191 1471 Fragments arranged by size: 1968 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5665 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5665 SmaI CCC'GGG Cuts at: 1872 2992 1872 Size: 1120 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2276 2936 3769 4670 4932 820 Size: 197 926 333 660 833 901 262 1553 Fragments arranged by size: 1553 926 901 833 660 333 262 197 SnaBI TAC'GTA Cuts at: 493 3456 493 Size: 2963 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5665 SphI G_CATG'C Cuts at: 1132 3023 1132 Size: 1891 3774 SspI AAT'ATT Cuts at: 5 52 3589 5 Size: 47 3537 2081 Fragments arranged by size: 3537 2081 47 StuI AGG'CCT Cuts at: 1517 2171 2720 1517 Size: 654 549 4462 Fragments arranged by size: 4462 654 549 StyI C'CwwG_G Cuts at: 513 1065 3104 513 Size: 552 2039 3074 Fragments arranged by size: 3074 2039 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3692 3709 3862 4047 4492 325 Size: 1255 2112 17 153 185 445 1498 Fragments arranged by size: 2112 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2112 3911 5623 Size: 274 80 33 51 1443 135 1799 1712 Cuts at: 5623 96 Size: 138 Fragments arranged by size: 1799 1712 1443 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2752 2784 5625 5657 2752 Size: 32 2841 32 2760 Fragments arranged by size: 2841 2760 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5665 XbaI T'CTAG_A Cuts at: 3001 3001 Size: 5665 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5665 XmaI C'CCGG_G Cuts at: 1870 2990 1870 Size: 1120 4545 XmnI GAAnn'nnTTC Cuts at: 2720 3794 2720 Size: 1074 4591 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3473 276 Size: 53 83 186 936 1939 2468 Fragments arranged by size: 2468 1939 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI