(Circular) (Six-base) MAPSORT of: pFN21AE6083.seq Check: 1665 from: 1 to: 5677 pFN21AE6083 5677 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3487 278 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2238 3020 1937 Size: 301 782 4594 Fragments arranged by size: 4594 782 301 Acc65I G'GTAC_C Cuts at: 2998 2998 Size: 5677 AclI AA'CG_TT Cuts at: 1563 3804 4177 1563 Size: 2241 373 3063 Fragments arranged by size: 3063 2241 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2745 2894 3740 5124 5392 1632 Size: 1113 149 846 1384 268 1917 Fragments arranged by size: 1917 1384 1113 846 268 149 AfeI AGC'GCT Cuts at: 2321 2321 Size: 5677 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5480 AflIII A'CryG_T Cuts at: 1203 4576 5553 1203 Size: 3373 977 1327 Fragments arranged by size: 3373 1327 977 AgeI A'CCGG_T Cuts at: 4508 4508 Size: 5677 AhdI GACnn_n'nnGTC Cuts at: 4406 4406 Size: 5677 AleI CACnn'nnGTG Cuts at: 1166 2504 1166 Size: 1338 4339 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5677 AlwNI CAG_nnn'CTG Cuts at: 2510 3420 4992 2510 Size: 910 1572 3195 Fragments arranged by size: 3195 1572 910 ApaLI G'TGCA_C Cuts at: 3734 4890 3734 Size: 1156 4521 ApoI r'AATT_y Cuts at: 1493 2048 2986 3212 3364 5473 1493 Size: 555 938 226 152 2109 1697 Fragments arranged by size: 2109 1697 938 555 226 152 AseI AT'TA_AT Cuts at: 160 1032 4231 160 Size: 872 3199 1606 Fragments arranged by size: 3199 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 AvaI C'yCGr_G Cuts at: 1870 1943 2083 3002 1870 Size: 73 140 919 4545 Fragments arranged by size: 4545 919 140 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2117 2150 2117 Size: 33 5644 BamHI G'GATC_C Cuts at: 3007 3374 3007 Size: 367 5310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2998 4453 618 Size: 325 217 627 1211 1455 1842 Fragments arranged by size: 1842 1455 1211 627 325 217 BanII G_rGCy'C Cuts at: 1391 2996 1391 Size: 1605 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4706 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3868 3902 1903 Size: 34 1931 34 3678 Fragments arranged by size: 3678 1931 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2381 3569 4779 2381 Size: 1188 1210 3279 Fragments arranged by size: 3279 1210 1188 BclI T'GATC_A Cuts at: 1548 1548 Size: 5677 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4288 136 Size: 107 122 71 631 605 2616 1525 Fragments arranged by size: 2616 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2972 5672 2972 Size: 2700 2977 BlpI GC'TnA_GC Cuts at: 3094 3094 Size: 5677 Bme1580I G_kGCm'C Cuts at: 1790 3738 4894 1790 Size: 1948 1156 2573 Fragments arranged by size: 2573 1948 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5677 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3408 4366 442 Size: 416 53 83 399 24 1991 958 1753 Fragments arranged by size: 1991 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5677 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2487 4337 1239 Size: 1248 1850 2579 Fragments arranged by size: 2579 1850 1248 Bpu10I CC'TnA_GC Cuts at: 2316 2744 2316 Size: 428 5249 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3802 4667 4965 3802 Size: 865 298 4514 Fragments arranged by size: 4514 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2062 4340 882 Size: 617 563 2278 2219 Fragments arranged by size: 2278 2219 617 563 BsaAI yAC'GTr Cuts at: 493 1204 3468 493 Size: 711 2264 2702 Fragments arranged by size: 2702 2264 711 BsaBI GATnn'nnATC Cuts at: 5671 5671 Size: 5677 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3484 3866 4501 275 Size: 53 83 186 936 1951 382 635 1451 Fragments arranged by size: 1951 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4110 4508 4782 4929 5340 4110 Size: 398 274 147 411 4447 Fragments arranged by size: 4447 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5452 Fragments arranged by size: 5452 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2366 1186 Size: 1180 4497 BseYI C'CCAG_C Cuts at: 2074 4880 5376 2074 Size: 2806 496 2375 Fragments arranged by size: 2806 2375 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5104 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3127 3888 4916 1540 Size: 275 186 1126 761 1028 2301 Fragments arranged by size: 2301 1126 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2570 2750 3738 3823 4894 2570 Size: 180 988 85 1071 3353 Fragments arranged by size: 3353 1071 988 180 85 BsmI GAATG_Cn' Cuts at: 2088 3188 3281 2088 Size: 1100 93 4484 Fragments arranged by size: 4484 1100 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2570 2750 2996 3738 3823 4894 1391 Size: 399 780 180 246 742 85 1071 2174 Fragments arranged by size: 2174 1071 780 742 399 246 180 85 BspEI T'CCGG_A Cuts at: 5340 5340 Size: 5677 BspHI T'CATG_A Cuts at: 3566 5296 3566 Size: 1730 3947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3032 844 Size: 2188 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3564 1394 Size: 429 1741 3507 Fragments arranged by size: 3507 1741 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4172 4346 65 Size: 1183 2924 174 1396 Fragments arranged by size: 2924 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4321 4508 1700 Size: 110 2511 187 2869 Fragments arranged by size: 2869 2511 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5677 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5677 BssSI C'ACGA_G Cuts at: 3737 4749 3737 Size: 1012 4665 BstAPI GCAn_nnn'nTGC Cuts at: 794 2150 794 Size: 1356 4321 BstBI TT'CG_AA Cuts at: 4481 4481 Size: 5677 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5677 BstYI r'GATC_y Cuts at: 1972 2271 2972 3007 3374 3759 3776 5228 5314 Size: 299 701 35 367 385 17 1452 86 Cuts at: 5314 5326 5672 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 701 385 367 346 299 86 35 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5125 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4938 Fragments arranged by size: 4938 683 56 BtsI GCAGTG_nn' Cuts at: 2557 3193 3987 4007 2557 Size: 636 794 20 4227 Fragments arranged by size: 4227 794 636 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5642 DraI TTT'AAA Cuts at: 2981 2981 Size: 5677 DraIII CAC_nnn'GTG Cuts at: 2528 2528 Size: 5677 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4684 809 Size: 507 3368 1802 Fragments arranged by size: 3368 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2277 3124 Size: 54 1425 50 275 18 420 27 847 Cuts at: 3124 4013 5642 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 847 420 275 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3124 1537 Size: 275 1312 4090 Fragments arranged by size: 4090 1312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2424 3607 1353 Size: 1071 1183 3423 Fragments arranged by size: 3423 1183 1071 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4264 4638 4784 387 Size: 1064 138 2675 374 146 1280 Fragments arranged by size: 2675 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2487 2745 2894 3740 4337 5124 5392 Size: 393 855 258 149 846 597 787 268 Cuts at: 5392 1239 Size: 1524 Fragments arranged by size: 1524 855 846 787 597 393 268 258 149 EcoO109I rG'GnC_Cy Cuts at: 2740 2740 Size: 5677 EcoRI G'AATT_C Cuts at: 2986 3364 2986 Size: 378 5299 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2610 2610 Size: 5677 FspI TGC'GCA Cuts at: 4183 4183 Size: 5677 HaeII r_GCGC'y Cuts at: 1678 2323 4824 1678 Size: 645 2501 2531 Fragments arranged by size: 2531 2501 645 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2522 2554 4318 4350 4392 Size: 32 135 32 950 32 1764 32 42 Cuts at: 4392 4424 1373 Size: 32 2626 Fragments arranged by size: 2626 1764 950 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3021 3267 669 Size: 988 281 1083 246 3079 Fragments arranged by size: 3079 1083 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5677 HpaI GTT'AAC Cuts at: 3267 3267 Size: 5677 KpnI G_GTAC'C Cuts at: 3002 3002 Size: 5677 MfeI C'AATT_G Cuts at: 2433 3276 2433 Size: 843 4834 MluI A'CGCG_T Cuts at: 5553 5553 Size: 5677 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4791 4975 1305 Size: 3486 184 2007 Fragments arranged by size: 3486 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2252 2279 10 Size: 54 1425 343 420 27 3408 Fragments arranged by size: 3408 1425 420 343 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 2504 3635 3994 4153 518 Size: 648 1338 1131 359 159 2042 Fragments arranged by size: 2042 1338 1131 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2097 2723 3093 3770 4918 5163 1606 Size: 491 626 370 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 626 491 370 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5125 NdeI CA'TA_TG Cuts at: 387 2013 387 Size: 1626 4051 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5677 NotI GC'GGCC_GC Cuts at: 3124 3124 Size: 5677 NspI r_CATG'y Cuts at: 1132 1229 3035 4580 1132 Size: 97 1806 1545 2229 Fragments arranged by size: 2229 1806 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5677 PciI A'CATG_T Cuts at: 4576 4576 Size: 5677 PflMI CCAn_nnn'nTGG Cuts at: 1108 2286 1108 Size: 1178 4499 PmeI GTTT'AAAC Cuts at: 2981 2981 Size: 5677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3746 3778 5259 5291 3746 Size: 32 1481 32 4132 Fragments arranged by size: 4132 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2244 1613 Size: 631 5046 PsiI TTA'TAA Cuts at: 3247 3247 Size: 5677 PstI C_TGCA'G Cuts at: 830 2734 3029 830 Size: 1904 295 3478 Fragments arranged by size: 3478 1904 295 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5677 PvuII CAG'CTG Cuts at: 2097 2723 2097 Size: 626 5051 SalI G'TCGA_C Cuts at: 1936 3019 1936 Size: 1083 4594 SbfI CC_TGCA'GG Cuts at: 3029 3029 Size: 5677 ScaI AGT'ACT Cuts at: 3925 5637 3925 Size: 1712 3965 SfcI C'TryA_G Cuts at: 826 1045 2730 2895 3025 4160 4841 5032 826 Size: 219 1685 165 130 1135 681 191 1471 Fragments arranged by size: 1685 1471 1135 681 219 191 165 130 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5677 SmaI CCC'GGG Cuts at: 1872 2085 3004 1872 Size: 213 919 4545 Fragments arranged by size: 4545 919 213 SmlI C'TyrA_G Cuts at: 820 1017 1943 3781 4682 4944 820 Size: 197 926 1838 901 262 1553 Fragments arranged by size: 1838 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3468 493 Size: 2975 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5677 SphI G_CATG'C Cuts at: 1132 3035 1132 Size: 1903 3774 SspI AAT'ATT Cuts at: 5 52 3601 5 Size: 47 3549 2081 Fragments arranged by size: 3549 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5677 StyI C'CwwG_G Cuts at: 513 1065 2218 2298 2575 2736 3116 513 Size: 552 1153 80 277 161 380 3074 Fragments arranged by size: 3074 1153 552 380 277 161 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3704 3721 3874 4059 4504 325 Size: 1255 2124 17 153 185 445 1498 Fragments arranged by size: 2124 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2697 2704 3923 Size: 274 80 33 51 1443 720 7 1219 Cuts at: 3923 5635 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1219 720 274 138 80 51 33 7 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5637 5669 5637 Size: 32 5645 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5677 XbaI T'CTAG_A Cuts at: 3013 3013 Size: 5677 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5677 XmaI C'CCGG_G Cuts at: 1870 2083 3002 1870 Size: 213 919 4545 Fragments arranged by size: 4545 919 213 XmnI GAAnn'nnTTC Cuts at: 3806 3806 Size: 5677 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3485 276 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI