################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HhaI HinP1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 962 GAATTT [GA]^AATT[CT] AcyI 754 GGCGTC G[GA]^CG[CT]C AfaI 411 GTAC GT^AC AflIII 380 ACACGT A^C[GA][CT]GT Alw44I 284 GTGCAC G^TGCAC AlwI 176 GGATC GGATC(4/5) ApaLI 284 GTGCAC G^TGCAC ApoI 962 GAATTT [GA]^AATT[CT] AspI 672 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 404 CCCGGG C^[CT]CG[GA]G BbvCI 444 GCTGAGG CCTCAGC(-5/-2) BclI 276 TGATCA T^GATCA BglI 204 GCCTAATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 699 AGATCT A^GATCT BsaHI 754 GGCGTC G[GA]^CG[CT]C BsaI 356 GGTCTC GGTCTC(1/5) BsgI 664 GTGCAG GTGCAG(16/14) BsmFI 678 GTCCC GGGAC(10/14) BsmI 848 GAATGC GAATGC(1/-1) BsoBI 404 CCCGGG C^[CT]CG[GA]G BspMI 40 ACCTGC ACCTGC(4/8) BsrBI 352 GAGCGG CCGCTC(-3/-3) BsrDI 590 GCAATG GCAATG(2/0) BstAPI 708 GCATCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 699 AGATCT [GA]^GATC[CT] DrdI 325 GACGGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 90 CGGCCA [CT]^GGCC[GA] EcoT14I 291 CCATGG C^C[AT][AT]GG FbaI 276 TGATCA T^GATCA Hin1I 754 GGCGTC G[GA]^CG[CT]C HindIII 500 AAGCTT A^AGCTT MflI 699 AGATCT [GA]^GATC[CT] NcoI 291 CCATGG C^CATGG PflFI 672 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 513 GACTC GAGTC(4/5) PshAI 873 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 715 CTGCAG CTGCA^G RsaI 411 GTAC GT^AC SfcI 715 CTGCAG C^T[GA][CT]AG SmaI 404 CCCGGG CCC^GGG SphI 109 GCATGC GCATG^C StyI 291 CCATGG C^C[AT][AT]GG TaqI 828 TCGA T^CGA Tsp509I 963 AATT ^AATT Tth111I 672 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 828 TCGA T^CGA XhoII 699 AGATCT [GA]^GATC[CT] XmaCI 404 CCCGGG C^CCGGG XmaI 404 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AlwNI 96 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 676 CAGTCCCTG Asp700I 622 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 796 GAAACTTTTC BbsI 510 GAAGAC GAAGAC(2/6) 622 GAAGAC BcnI 404 CCCGG CC^[GC]GG 405 CCGGG BfaI 142 CTAG C^TAG 853 CTAG BpuAI 510 GAAGAC GAAGAC(2/6) 622 GAAGAC BsmAI 566 GAGAC GTCTC(1/5) 357 GTCTC BstEII 36 GGTGACC G^GT[ACGT]ACC 373 GGTCACC BstF5I 570 CATCC GGATG(2/0) 750 GGATG BstPI 36 GGTGACC G^GT[ACGT]ACC 373 GGTCACC DsaI 291 CCATGG C^C[GA][CT]GG 369 CCGTGG EcoNI 387 CCTCACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 606 CCTGGAGAAGG EcoO65I 36 GGTGACC G^GT[ACGT]ACC 373 GGTCACC FokI 570 CATCC GGATG(9/13) 750 GGATG HaeIII 91 GGCC GG^CC 203 GGCC HapII 174 CCGG C^CGG 405 CCGG HgaI 884 GACGC GACGC(5/10) 755 GCGTC HpaII 174 CCGG C^CGG 405 CCGG HphI 36 GGTGA GGTGA(8/7) 375 TCACC MaeI 142 CTAG C^TAG 853 CTAG MseI 20 TTAA T^TAA 992 TTAA MspI 174 CCGG C^CGG 405 CCGG NciI 404 CCCGG CC^[GC]GG 405 CCGGG NspI 109 GCATGC [GA]CATG^[CT] 213 GCATGT SacI 688 GAGCTC GAGCT^C 830 GAGCTC SfaNI 441 GATGC GCATC(5/9) 708 GCATC TfiI 11 GAATC G^A[AT]TC 1003 GATTC Tru9I 20 TTAA T^TAA 992 TTAA TspRI 117 CACTG CA[GC]TG[ACGT][ACGT]^ 725 CAGTG XmnI 622 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 796 GAAACTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AluI AspHI BanII BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstNI BstXI Cac8I DdeI DpnII EarI EcoRII Fnu4HI HinfI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII Sau3AI ScrFI TseI Tsp45I