(Circular) (Six-base) MAPSORT of: pFN21AE3966.seq Check: 7886 from: 1 to: 5746 pFN21AE3966 5746 bp With 182 enzymes: SgfI * March 28, 2012 11:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3089 1937 Size: 1152 4594 Acc65I G'GTAC_C Cuts at: 3067 3067 Size: 5746 AclI AA'CG_TT Cuts at: 1563 3873 4246 1563 Size: 2310 373 3063 Fragments arranged by size: 3063 2310 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2493 2729 3809 5193 5461 1632 Size: 861 236 1080 1384 268 1917 Fragments arranged by size: 1917 1384 1080 861 268 236 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5549 AflIII A'CryG_T Cuts at: 1203 2385 4645 5622 1203 Size: 1182 2260 977 1327 Fragments arranged by size: 2260 1327 1182 977 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 4475 4475 Size: 5746 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2618 1184 Size: 1434 4312 AlwNI CAG_nnn'CTG Cuts at: 2106 2686 3489 5061 2106 Size: 580 803 1572 2791 Fragments arranged by size: 2791 1572 803 580 ApaLI G'TGCA_C Cuts at: 2289 3803 4959 2289 Size: 1514 1156 3076 Fragments arranged by size: 3076 1514 1156 ApoI r'AATT_y Cuts at: 1493 2967 3055 3281 3433 5542 1493 Size: 1474 88 226 152 2109 1697 Fragments arranged by size: 2109 1697 1474 226 152 88 AseI AT'TA_AT Cuts at: 160 1032 4300 160 Size: 872 3268 1606 Fragments arranged by size: 3268 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 2409 3071 1870 Size: 73 466 662 4545 Fragments arranged by size: 4545 662 466 73 BamHI G'GATC_C Cuts at: 3076 3443 3076 Size: 367 5379 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3067 4522 618 Size: 325 217 627 1280 1455 1842 Fragments arranged by size: 1842 1455 1280 627 325 217 BanII G_rGCy'C Cuts at: 1391 2264 2697 2839 3065 1391 Size: 873 433 142 226 4072 Fragments arranged by size: 4072 873 433 226 142 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2522 2634 928 Size: 971 623 112 4040 Fragments arranged by size: 4040 971 623 112 BbvCI CC'TCA_GC Cuts at: 2450 2450 Size: 5746 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3937 3971 1903 Size: 34 2000 34 3678 Fragments arranged by size: 3678 2000 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3638 4848 3638 Size: 1210 4536 BclI T'GATC_A Cuts at: 1548 2281 1548 Size: 733 5013 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2215 4357 136 Size: 107 122 71 631 605 543 2142 1525 Fragments arranged by size: 2142 1525 631 605 543 122 107 71 BglII A'GATC_T Cuts at: 2704 5741 2704 Size: 3037 2709 BlpI GC'TnA_GC Cuts at: 3163 3163 Size: 5746 Bme1580I G_kGCm'C Cuts at: 1790 2293 3807 4963 1790 Size: 503 1514 1156 2573 Fragments arranged by size: 2573 1514 1156 503 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5746 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2593 2674 2905 Size: 416 53 83 399 24 1176 81 231 Cuts at: 2905 3477 4435 442 Size: 572 958 1753 Fragments arranged by size: 1753 1176 958 572 416 399 231 83 81 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2716 2716 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2414 2462 2633 2729 4406 1239 Size: 1175 48 171 96 1677 2579 Fragments arranged by size: 2579 1677 1175 171 96 48 Bpu10I CC'TnA_GC Cuts at: 2081 2243 2450 2081 Size: 162 207 5377 Fragments arranged by size: 5377 207 162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3871 4736 5034 3871 Size: 865 298 4583 Fragments arranged by size: 4583 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2367 4409 882 Size: 617 868 2042 2219 Fragments arranged by size: 2219 2042 868 617 BsaAI yAC'GTr Cuts at: 493 1204 3537 493 Size: 711 2333 2702 Fragments arranged by size: 2702 2333 711 BsaBI GATnn'nnATC Cuts at: 5740 5740 Size: 5746 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2760 3553 3935 4570 Size: 53 83 186 936 1227 793 382 635 Cuts at: 4570 275 Size: 1451 Fragments arranged by size: 1451 1227 936 793 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4179 4577 4851 4998 5409 4179 Size: 398 274 147 411 4516 Fragments arranged by size: 4516 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2240 2270 1178 Size: 30 165 30 837 30 4654 Fragments arranged by size: 4654 837 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2414 2603 2636 1186 Size: 865 363 189 33 4296 Fragments arranged by size: 4296 865 363 189 33 BseYI C'CCAG_C Cuts at: 4949 5445 4949 Size: 496 5250 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2690 1163 Size: 573 954 4219 Fragments arranged by size: 4219 954 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3196 3957 4985 1540 Size: 275 186 1195 761 1028 2301 Fragments arranged by size: 2301 1195 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2293 2373 2697 2839 3807 3892 4963 2293 Size: 80 324 142 968 85 1071 3076 Fragments arranged by size: 3076 1071 968 324 142 85 80 BsmI GAATG_Cn' Cuts at: 2859 3257 3350 2859 Size: 398 93 5255 Fragments arranged by size: 5255 398 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2264 2293 2373 2697 2839 3065 3807 Size: 399 474 29 80 324 142 226 742 Cuts at: 3807 3892 4963 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 474 399 324 226 142 85 80 29 BspEI T'CCGG_A Cuts at: 5409 5409 Size: 5746 BspHI T'CATG_A Cuts at: 3635 5365 3635 Size: 1730 4016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2054 3101 844 Size: 1210 1047 3489 Fragments arranged by size: 3489 1210 1047 BsrBI CCG'CTC Cuts at: 1394 1823 2359 3633 1394 Size: 429 536 1274 3507 Fragments arranged by size: 3507 1274 536 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2602 4241 4415 65 Size: 1183 1354 1639 174 1396 Fragments arranged by size: 1639 1396 1354 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4390 4577 1700 Size: 110 2580 187 2869 Fragments arranged by size: 2869 2580 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5746 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5746 BssSI C'ACGA_G Cuts at: 3806 4818 3806 Size: 1012 4734 BstAPI GCAn_nnn'nTGC Cuts at: 794 2719 794 Size: 1925 3821 BstBI TT'CG_AA Cuts at: 4550 4550 Size: 5746 BstEII G'GTnAC_C Cuts at: 1184 2041 2378 1184 Size: 857 337 4552 Fragments arranged by size: 4552 857 337 BstXI CCAn_nnnn'nTGG Cuts at: 2059 2233 2548 2059 Size: 174 315 5257 Fragments arranged by size: 5257 315 174 BstYI r'GATC_y Cuts at: 1972 2704 3076 3443 3828 3845 5297 5383 5395 Size: 732 372 367 385 17 1452 86 12 Cuts at: 5395 5741 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 732 385 372 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2296 2374 513 Size: 552 939 292 78 3885 Fragments arranged by size: 3885 939 552 292 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5007 Fragments arranged by size: 5007 683 56 BtsI GCAGTG_nn' Cuts at: 2121 3262 4056 4076 2121 Size: 1141 794 20 3791 Fragments arranged by size: 3791 1141 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5711 DraI TTT'AAA Cuts at: 3050 3050 Size: 5746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2336 4753 809 Size: 507 1020 2417 1802 Fragments arranged by size: 2417 1802 1020 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2095 3193 4082 Size: 54 1425 50 275 18 265 1098 889 Cuts at: 4082 5711 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1098 889 275 265 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3193 1537 Size: 275 1381 4090 Fragments arranged by size: 4090 1381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2321 2469 2493 3676 1353 Size: 968 148 24 1183 3423 Fragments arranged by size: 3423 1183 968 148 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4333 4707 4853 387 Size: 1064 138 2744 374 146 1280 Fragments arranged by size: 2744 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2695 2837 2695 Size: 142 5604 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2414 2462 2493 2633 2729 3809 4406 Size: 393 782 48 31 140 96 1080 597 Cuts at: 4406 5193 5461 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1080 787 782 597 393 268 140 96 48 31 EcoNI CCTnn'n_nnAGG Cuts at: 2396 2615 2396 Size: 219 5527 EcoRI G'AATT_C Cuts at: 3055 3433 3055 Size: 378 5368 FspI TGC'GCA Cuts at: 4252 4252 Size: 5746 HaeII r_GCGC'y Cuts at: 1678 4893 1678 Size: 3215 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2682 2714 4387 4419 4461 Size: 32 135 32 1110 32 1673 32 42 Cuts at: 4461 4493 1373 Size: 32 2626 Fragments arranged by size: 2626 1673 1110 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3090 3336 669 Size: 988 281 1152 246 3079 Fragments arranged by size: 3079 1152 988 281 246 HindIII A'AGCT_T Cuts at: 748 2505 748 Size: 1757 3989 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KpnI G_GTAC'C Cuts at: 3071 3071 Size: 5746 MfeI C'AATT_G Cuts at: 3345 3345 Size: 5746 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2460 2911 4860 5044 1305 Size: 1155 451 1949 184 2007 Fragments arranged by size: 2007 1949 1155 451 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3924 Fragments arranged by size: 3924 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3704 4063 4222 518 Size: 648 2538 359 159 2042 Fragments arranged by size: 2538 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3162 3839 4987 5232 1606 Size: 1556 677 1148 245 2120 Fragments arranged by size: 2120 1556 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2296 513 Size: 552 939 292 3963 Fragments arranged by size: 3963 939 552 292 NdeI CA'TA_TG Cuts at: 387 387 Size: 5746 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 3193 3193 Size: 5746 NspI r_CATG'y Cuts at: 1132 1229 2118 2222 3104 4649 1132 Size: 97 889 104 882 1545 2229 Fragments arranged by size: 2229 1545 889 882 104 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5746 PciI A'CATG_T Cuts at: 4645 4645 Size: 5746 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5746 PfoI T'CCnGG_A Cuts at: 2510 2577 2510 Size: 67 5679 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3815 3847 5328 5360 3815 Size: 32 1481 32 4201 Fragments arranged by size: 4201 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2882 1613 Size: 1269 4477 PsiI TTA'TAA Cuts at: 3316 3316 Size: 5746 PstI C_TGCA'G Cuts at: 830 2724 3098 830 Size: 1894 374 3478 Fragments arranged by size: 3478 1894 374 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 SacI G_AGCT'C Cuts at: 2697 2839 2697 Size: 142 5604 SalI G'TCGA_C Cuts at: 1936 3088 1936 Size: 1152 4594 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 3994 5706 3994 Size: 1712 4034 SfcI C'TryA_G Cuts at: 826 1045 2720 3094 4229 4910 5101 826 Size: 219 1675 374 1135 681 191 1471 Fragments arranged by size: 1675 1471 1135 681 374 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5746 SmaI CCC'GGG Cuts at: 1872 2411 3073 1872 Size: 539 662 4545 Fragments arranged by size: 4545 662 539 SmlI C'TyrA_G Cuts at: 820 1017 1943 3850 4751 5013 820 Size: 197 926 1907 901 262 1553 Fragments arranged by size: 1907 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 2118 3104 1132 Size: 986 986 3774 Fragments arranged by size: 3774 986 986 SspI AAT'ATT Cuts at: 5 52 3670 5 Size: 47 3618 2081 Fragments arranged by size: 3618 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5746 StyI C'CwwG_G Cuts at: 513 1065 2004 2296 3185 513 Size: 552 939 292 889 3074 Fragments arranged by size: 3074 939 889 552 292 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3773 3790 3943 4128 4573 325 Size: 1255 2193 17 153 185 445 1498 Fragments arranged by size: 2193 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3992 5704 96 Size: 274 80 33 51 1443 2015 1712 138 Fragments arranged by size: 2015 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2123 2155 5706 5738 2123 Size: 32 3551 32 2131 Fragments arranged by size: 3551 2131 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2680 1316 Size: 1364 4382 XbaI T'CTAG_A Cuts at: 3082 3082 Size: 5746 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5746 XmaI C'CCGG_G Cuts at: 1870 2409 3071 1870 Size: 539 662 4545 Fragments arranged by size: 4545 662 539 XmnI GAAnn'nnTTC Cuts at: 2631 2805 3875 2631 Size: 174 1070 4502 Fragments arranged by size: 4502 1070 174 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI