Restriction map for
pFN21AE4115
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AvaI
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraIII EagI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI StuI Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AflIII 386 ACACGT A^C[GA][CT]GT
AhdI 1443 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 789 CCTGAGG CC^T[ACGT]AGG
ApaI 258 GGGCCC GGGCC^C
AspEI 1443 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 678 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1089 GAAGAC GAAGAC(2/6)
BciVI 1198 GGATAC GTATCC(6/5)
BfaI 580 CTAG C^TAG
BglI 207 GCCTAATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 843 AGATCT A^GATCT
BpuAI 1089 GAAGAC GAAGAC(2/6)
BsaI 362 GGTCTC GGTCTC(1/5)
BsaWI 956 ACCGGA [AT]^CCGG[AT]
BsgI 290 GTGCAG GTGCAG(16/14)
BsmFI 1128 GGGAC GGGAC(10/14)
BsrBI 358 GAGCGG CCGCTC(-3/-3)
BssSI 394 CTCGTG CACGAG(-5/-1)
Bsu36I 789 CCTGAGG CC^T[ACGT]AGG
DraI 933 TTTAAA TTT^AAA
Eam1105I 1443 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 789 CCTGAGG CC^T[ACGT]AGG
EcoNI 393 CCTCGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 957 CCGG C^CGG
HpaII 957 CCGG C^CGG
MaeI 580 CTAG C^TAG
MspI 957 CCGG C^CGG
PflFI 678 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 436 GAGTC GAGTC(4/5)
PpuMI 1128 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 879 GACAGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 315 GCTCTTC GCTCTTC(1/4)
ScaI 12 AGTACT AGT^ACT
SspI 1382 AATATT AAT^ATT
SwaI 932 ATTTAAAT ATTT^AAAT
TaqI 717 TCGA T^CGA
Tth111I 678 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 717 TCGA T^CGA
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 96 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
682 CAGTCGCTG
BamHI 545 GGATCC G^GATCC
1100 GGATCC
BspMI 40 ACCTGC ACCTGC(4/8)
200 ACCTGC
BstEII 36 GGTGACC G^GT[ACGT]ACC
379 GGTCACC
BstPI 36 GGTGACC G^GT[ACGT]ACC
379 GGTCACC
Cac8I 109 GCCTGC GC[ACGT]^[ACGT]GC
347 GCTGGC
DrdI 995 GACCTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1021 GACTGTAGTGTC
EcoO65I 36 GGTGACC G^GT[ACGT]ACC
379 GGTCACC
EcoT14I 297 CCATGG C^C[AT][AT]GG
1353 CCATGG
HgaI 714 GCGTC GACGC(5/10)
1449 GCGTC
HincII 729 GTCAAC GT[CT]^[GA]AC
901 GTTGAC
HindII 729 GTCAAC GT[CT]^[GA]AC
901 GTTGAC
MaeII 388 ACGT A^CGT
905 ACGT
MslI 1222 CATAACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1275 CATGACAGTG
MspA1I 271 CAGCTG C[AC]G^C[GT]G
565 CAGCTG
NcoI 297 CCATGG C^CATGG
1353 CCATGG
PstI 310 CTGCAG CTGCA^G
745 CTGCAG
PvuII 271 CAGCTG CAG^CTG
565 CAGCTG
SacI 58 GAGCTC GAGCT^C
694 GAGCTC
StyI 297 CCATGG C^C[AT][AT]GG
1353 CCATGG
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BalI BanII BbvI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII