(Circular) (Six-base) MAPSORT of: pFN21AE4115.seq Check: 4255 from: 1 to: 6169 pFN21AE4115 6169 bp With 182 enzymes: SgfI * July 4, 2020 18:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3979 278 Size: 53 83 186 936 2443 2468 Fragments arranged by size: 2468 2443 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3512 1937 Size: 1575 4594 Acc65I G'GTAC_C Cuts at: 3490 3490 Size: 6169 AclI AA'CG_TT Cuts at: 1563 4296 4669 1563 Size: 2733 373 3063 Fragments arranged by size: 3063 2733 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2477 4232 5616 5884 1632 Size: 845 1755 1384 268 1917 Fragments arranged by size: 1917 1755 1384 845 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5972 AflIII A'CryG_T Cuts at: 1203 2391 5068 6045 1203 Size: 1188 2677 977 1327 Fragments arranged by size: 2677 1327 1188 977 AgeI A'CCGG_T Cuts at: 5000 5000 Size: 6169 AhdI GACnn_n'nnGTC Cuts at: 3453 4898 3453 Size: 1445 4724 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6169 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6169 AlwNI CAG_nnn'CTG Cuts at: 2106 2692 3912 5484 2106 Size: 586 1220 1572 2791 Fragments arranged by size: 2791 1572 1220 586 ApaI G_GGCC'C Cuts at: 2267 2267 Size: 6169 ApaLI G'TGCA_C Cuts at: 4226 5382 4226 Size: 1156 5013 ApoI r'AATT_y Cuts at: 1493 3478 3704 3856 5965 1493 Size: 1985 226 152 2109 1697 Fragments arranged by size: 2109 1985 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4723 160 Size: 872 3691 1606 Fragments arranged by size: 3691 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 AvaI C'yCGr_G Cuts at: 1870 1943 3494 1870 Size: 73 1551 4545 Fragments arranged by size: 4545 1551 73 BamHI G'GATC_C Cuts at: 2550 3105 3499 3866 2550 Size: 555 394 367 4853 Fragments arranged by size: 4853 555 394 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3490 4945 618 Size: 325 217 627 1703 1455 1842 Fragments arranged by size: 1842 1703 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2067 2267 2703 2845 3044 3488 1391 Size: 676 200 436 142 199 444 4072 Fragments arranged by size: 4072 676 444 436 200 199 142 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3101 928 Size: 971 1202 3996 Fragments arranged by size: 3996 1202 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4360 4394 1903 Size: 34 2423 34 3678 Fragments arranged by size: 3678 2423 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3197 4061 5271 3197 Size: 864 1210 4095 Fragments arranged by size: 4095 1210 864 BclI T'GATC_A Cuts at: 1548 1548 Size: 6169 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2218 4780 136 Size: 107 122 71 631 605 546 2562 1525 Fragments arranged by size: 2562 1525 631 605 546 122 107 71 BglII A'GATC_T Cuts at: 2848 6164 2848 Size: 3316 2853 BlpI GC'TnA_GC Cuts at: 3586 3586 Size: 6169 Bme1580I G_kGCm'C Cuts at: 1790 2267 3291 4230 5386 1790 Size: 477 1024 939 1156 2573 Fragments arranged by size: 2573 1156 1024 939 477 BmgBI CAC'GTC Cuts at: 1318 2911 1318 Size: 1593 4576 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2680 2911 3082 Size: 416 53 83 399 24 1263 231 171 Cuts at: 3082 3140 3217 3900 4858 442 Size: 58 77 683 958 1753 Fragments arranged by size: 1753 1263 958 683 416 399 231 171 83 77 58 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6169 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2722 2722 Size: 6169 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2735 3166 4829 1239 Size: 842 654 431 1663 2579 Fragments arranged by size: 2579 1663 842 654 431 Bpu10I CC'TnA_GC Cuts at: 2081 2081 Size: 6169 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4294 5159 5457 4294 Size: 865 298 5006 Fragments arranged by size: 5006 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2373 4832 882 Size: 617 874 2459 2219 Fragments arranged by size: 2459 2219 874 617 BsaAI yAC'GTr Cuts at: 493 1204 3960 493 Size: 711 2756 2702 Fragments arranged by size: 2756 2702 711 BsaBI GATnn'nnATC Cuts at: 6163 6163 Size: 6169 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3976 4358 4993 275 Size: 53 83 186 936 2443 382 635 1451 Fragments arranged by size: 2443 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2961 4602 5000 5274 5421 5832 2961 Size: 1641 398 274 147 411 3298 Fragments arranged by size: 3298 1641 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2976 3006 1178 Size: 30 165 30 1573 30 4341 Fragments arranged by size: 4341 1573 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2420 2480 3046 1186 Size: 1234 60 566 4309 Fragments arranged by size: 4309 1234 566 60 BseYI C'CCAG_C Cuts at: 3045 5372 5868 3045 Size: 2327 496 3346 Fragments arranged by size: 3346 2327 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2316 1163 Size: 573 580 5016 Fragments arranged by size: 5016 580 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3619 4380 5408 1540 Size: 275 186 1618 761 1028 2301 Fragments arranged by size: 2301 1618 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2067 2379 2703 4230 4315 5386 2067 Size: 312 324 1527 85 1071 2850 Fragments arranged by size: 2850 1527 1071 324 312 85 BsmI GAATG_Cn' Cuts at: 3680 3773 3680 Size: 93 6076 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2267 2379 2703 2845 3044 3291 Size: 399 277 200 112 324 142 199 247 Cuts at: 3291 3488 4230 4315 5386 1391 Size: 197 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 324 277 247 200 199 197 142 112 85 BspEI T'CCGG_A Cuts at: 5832 5832 Size: 6169 BspHI T'CATG_A Cuts at: 4058 5788 4058 Size: 1730 4439 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2054 2214 3524 844 Size: 1210 160 1310 3489 Fragments arranged by size: 3489 1310 1210 160 BsrBI CCG'CTC Cuts at: 1394 1823 2365 4056 1394 Size: 429 542 1691 3507 Fragments arranged by size: 3507 1691 542 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4664 4838 65 Size: 1183 3416 174 1396 Fragments arranged by size: 3416 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4813 5000 1700 Size: 110 3003 187 2869 Fragments arranged by size: 3003 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6169 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6169 BssSI C'ACGA_G Cuts at: 2399 4229 5241 2399 Size: 1830 1012 3327 Fragments arranged by size: 3327 1830 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6169 BstBI TT'CG_AA Cuts at: 4973 4973 Size: 6169 BstEII G'GTnAC_C Cuts at: 1184 2041 2384 1184 Size: 857 343 4969 Fragments arranged by size: 4969 857 343 BstYI r'GATC_y Cuts at: 1972 2550 2710 2848 3105 3499 3866 4251 4268 Size: 578 160 138 257 394 367 385 17 Cuts at: 4268 5720 5806 5818 6164 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 578 394 385 367 346 257 160 138 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2795 2795 Size: 6169 BtgI C'CryG_G Cuts at: 513 1065 2302 2380 3358 513 Size: 552 1237 78 978 3324 Fragments arranged by size: 3324 1237 978 552 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5430 Fragments arranged by size: 5430 683 56 BtsI GCAGTG_nn' Cuts at: 3685 4479 4499 3685 Size: 794 20 5355 Fragments arranged by size: 5355 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6169 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6134 DraI TTT'AAA Cuts at: 2940 3473 2940 Size: 533 5636 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3006 3032 5176 809 Size: 507 1690 26 2144 1802 Fragments arranged by size: 2144 1802 1690 507 26 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2095 2226 3087 Size: 54 1425 50 275 18 265 131 861 Cuts at: 3087 3616 4505 6134 8 Size: 529 889 1629 43 Fragments arranged by size: 1629 1425 889 861 529 275 265 131 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3616 1537 Size: 275 1804 4090 Fragments arranged by size: 4090 1804 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2031 2327 2622 2649 4099 1353 Size: 678 296 295 27 1450 3423 Fragments arranged by size: 3423 1450 678 296 295 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4756 5130 5276 387 Size: 1064 138 3167 374 146 1280 Fragments arranged by size: 3167 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2065 2701 2065 Size: 636 5533 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2477 2735 3166 4232 4829 5616 Size: 393 449 396 258 431 1066 597 787 Cuts at: 5616 5884 1239 Size: 268 1524 Fragments arranged by size: 1524 1066 787 597 449 431 396 393 268 258 EcoNI CCTnn'n_nnAGG Cuts at: 2402 2402 Size: 6169 EcoO109I rG'GnC_Cy Cuts at: 2264 3134 3243 2264 Size: 870 109 5190 Fragments arranged by size: 5190 870 109 EcoRI G'AATT_C Cuts at: 3478 3856 3478 Size: 378 5791 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2306 2306 Size: 6169 FspI TGC'GCA Cuts at: 4675 4675 Size: 6169 HaeII r_GCGC'y Cuts at: 1678 5316 1678 Size: 3638 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2535 2567 2688 2720 2905 Size: 32 135 32 963 32 121 32 185 Cuts at: 2905 2937 3439 3471 4810 4842 4884 4916 1373 Size: 32 502 32 1339 32 42 32 2626 Fragments arranged by size: 2626 1339 963 502 185 135 121 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2736 2908 3513 3759 669 Size: 988 281 798 172 605 246 3079 Fragments arranged by size: 3079 988 798 605 281 246 172 HindIII A'AGCT_T Cuts at: 748 748 Size: 6169 HpaI GTT'AAC Cuts at: 3759 3759 Size: 6169 KpnI G_GTAC'C Cuts at: 3494 3494 Size: 6169 MfeI C'AATT_G Cuts at: 3768 3768 Size: 6169 MluI A'CGCG_T Cuts at: 6045 6045 Size: 6169 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5283 5467 1305 Size: 3978 184 2007 Fragments arranged by size: 3978 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2097 2228 3089 10 Size: 54 1425 343 265 131 861 3090 Fragments arranged by size: 3090 1425 861 343 265 131 54 MslI CAynn'nnrTG Cuts at: 518 1166 3231 3284 4127 4486 4645 518 Size: 648 2065 53 843 359 159 2042 Fragments arranged by size: 2065 2042 843 648 359 159 53 MspA1I CmG'CkG Cuts at: 1606 2278 2572 3585 4262 5410 5655 1606 Size: 672 294 1013 677 1148 245 2120 Fragments arranged by size: 2120 1148 1013 677 672 294 245 NcoI C'CATG_G Cuts at: 513 1065 2302 3358 513 Size: 552 1237 1056 3324 Fragments arranged by size: 3324 1237 1056 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6169 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6169 NotI GC'GGCC_GC Cuts at: 3616 3616 Size: 6169 NspI r_CATG'y Cuts at: 1132 1229 3527 5072 1132 Size: 97 2298 1545 2229 Fragments arranged by size: 2298 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2267 1348 Size: 919 5250 PciI A'CATG_T Cuts at: 5068 5068 Size: 6169 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6169 PfoI T'CCnGG_A Cuts at: 2516 2516 Size: 6169 PmeI GTTT'AAAC Cuts at: 3473 3473 Size: 6169 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4238 4270 5751 5783 4238 Size: 32 1481 32 4624 Fragments arranged by size: 4624 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3134 3134 Size: 6169 PshAI GACnn'nnGTC Cuts at: 1613 2888 1613 Size: 1275 4894 PsiI TTA'TAA Cuts at: 3345 3739 3345 Size: 394 5775 PspOMI G'GGCC_C Cuts at: 2263 2263 Size: 6169 PstI C_TGCA'G Cuts at: 830 2319 2754 3521 830 Size: 1489 435 767 3478 Fragments arranged by size: 3478 1489 767 435 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6169 PvuII CAG'CTG Cuts at: 2278 2572 2278 Size: 294 5875 SacI G_AGCT'C Cuts at: 2067 2703 2067 Size: 636 5533 SalI G'TCGA_C Cuts at: 1936 3511 1936 Size: 1575 4594 SapI GCTCTTCn'nnn_ Cuts at: 2327 2327 Size: 6169 SbfI CC_TGCA'GG Cuts at: 3521 3521 Size: 6169 ScaI AGT'ACT Cuts at: 2019 4417 6129 2019 Size: 2398 1712 2059 Fragments arranged by size: 2398 2059 1712 SfcI C'TryA_G Cuts at: 826 1045 2315 2750 3028 3517 4652 5333 5524 Size: 219 1270 435 278 489 1135 681 191 Cuts at: 5524 826 Size: 1471 Fragments arranged by size: 1471 1270 1135 681 489 435 278 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6169 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6169 SmaI CCC'GGG Cuts at: 1872 3496 1872 Size: 1624 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4273 5174 5436 820 Size: 197 926 2330 901 262 1553 Fragments arranged by size: 2330 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3960 493 Size: 3467 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6169 SphI G_CATG'C Cuts at: 1132 3527 1132 Size: 2395 3774 SspI AAT'ATT Cuts at: 5 52 3389 4093 5 Size: 47 3337 704 2081 Fragments arranged by size: 3337 2081 704 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6169 StyI C'CwwG_G Cuts at: 513 1065 2302 3358 3608 513 Size: 552 1237 1056 250 3074 Fragments arranged by size: 3074 1237 1056 552 250 SwaI ATTT'AAAT Cuts at: 2940 2940 Size: 6169 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3199 4196 4213 4366 4551 4996 325 Size: 1255 1619 997 17 153 185 445 1498 Fragments arranged by size: 1619 1498 1255 997 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2017 4415 6127 Size: 274 80 33 51 1443 40 2398 1712 Cuts at: 6127 96 Size: 138 Fragments arranged by size: 2398 1712 1443 274 138 80 51 40 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3195 3227 6129 6161 3195 Size: 32 2902 32 3203 Fragments arranged by size: 3203 2902 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2686 1316 Size: 1370 4799 XbaI T'CTAG_A Cuts at: 3505 3505 Size: 6169 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6169 XmaI C'CCGG_G Cuts at: 1870 3494 1870 Size: 1624 4545 XmnI GAAnn'nnTTC Cuts at: 4298 4298 Size: 6169 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3977 276 Size: 53 83 186 936 2443 2468 Fragments arranged by size: 2468 2443 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI